49 results about "Orthotopic transplantation" patented technology

The invention belongs to the biological medicine field, and discloses a targeted boron preparation and a preparation method and application. The target boron preparation comprises polyamidoamine-amine dendrimers, epidermal growth factor receptor antibody and polyhedral boranes, the polyamide amine dendrimers are connected with the epidermal growth factor receptor antibody, and are internally loaded with the polyhedral boranes. The targeted boron preparation is high in boron content, can meet the requirements of BNCT (boron neutron capture therapy) on the boron atom dose, is well targeted to tumor cells, has good stability and low cell toxicity, and can be efficiently uptaken by human glioma U87MG cells of high surface expression EGFR (epidermal growth factor receptor). The target boron preparation can effectively improve the content of boron in orthotopic transplantation tumor nude mice tumor tissues, can significantly prolong orthotopic transplantation tumor nude mice lifetime by neutron irradiation, and is suitable for boron neutron capture therapy for glioma. The preparation method of the targeted boron preparation has the advantages of simple operation, and the prepared targeted boron preparation has good stability.

The invention discloses a manufacturing method of a biological tooth root bracket material. The method comprises the following steps of: flushing teeth with phosphate buffered saline (PBS) in a sterile state for three times; removing dental crown parts by grinding with a carborundum disc, and keeping tooth root parts; pulling pulp tissues out of the tooth roots and repeatedly flushing the inside of root canals with physiological saline; gradually expanding the inside of teeth with a dental expander and repeatedly flushing the inside of the root canals with 5 to 17 percent of ethylene diamine tetraacetic acid (EDTA) solution; grinding outer side tissues of the tooth roots by grinding with a dental turbine and constructing a biological tooth root profile as required so as to obtain a bracket with a specific shape; preserving the teeth in the PBS and treating in an ultrasonic oscillator for 3 to 8 times; performing stepped demineralization on a dentine with EDTA with different concentrations in a magnetic stirrer with the rotating speed of between 100 and 500 revolutions per minute so as to obtain a dentine substrate; and preserving the dentine substrate in double-antibody-containing PBS. The biological tooth root bracket material has a tooth root profile, the thickness of 1mm to 3mm, the length of 1cm to 2.5cm and certain tooth inducing capability, comprises a large number of proteins and factors related to the manufacturing of teeth, can release the proteins and factors continuously, can better solve the problem of induction micro-environment, and contributes to application to tooth socket intra-alveolar orthotopic transplantation.

The invention discloses a method for establishing a green fluorescence BALB/c nude mouse model. The method includes: selecting female C57BL/6-Tg (CAG-EGFP) mice and male Foxn1nu mice to perform hybrid to acquire F1 child generation hairy fluorescence mice, selecting the female mice, selecting the female mice having sexual maturation and the parental generation male Foxn1nu mice to perform mating (backcross) to acquire an F2 child generation; generating segregation of characters of the F2 child generation, selecting female hairy fluorescence mice and the parental generation male Foxn1nu mice to perform backcross, and completing a backcross cycle; performing the backcross cycle for ten times to acquire an F10 child generation, and establishing the green fluorescence BALB/c nude mouse model. The invention also discloses application of an animal model established by the method to a brain tumor transplanting model. The method uses a homogenous introduction method to establish the new strain model mice which can stably express EGFP, and can lay a firm foundation for human glioma orthotopic transplantation tracking and research on glioma and a micro environment thereof.

The invention provides a lymph gland targeted metastatic human hepatoma cell strain. The mother cell is a red fluorescent protein-expressed hepatoma cell HCCLM3-R. The human hepatoma cell strain is characterized in that the cell is named as lymph gland targeted metastatic human hepatoma cell strain HCCLM3-R-LnM1 according to the classified nomenclature with a preservation number of 3645, and has the following biological characteristics: the cell is polygonal epithelioid and is in adherent growth and loss of contact growth inhibition, is of a hypo-triploid karyotype, and has 50-58 of chromosomes each of which secretes AFP proteins. The establishing method comprises the following steps: using subcutaneous and liver orthotopic transplantation tumor models of a nude mouse to carry out spontaneous lymphatic metastasis screening, and then carrying out in-vitro cloning, amplification and purification on the lymphatic metastasis human hepatoma cell. The invention has wide application prospects and potential social and economic values in the basic research on hepatoma and the pre-clinic research on medicament.

The invention provides a method for constructing engineering arterial blood vessel in vivo by taking melt-spinning fiber as a skeleton. The method comprises the following steps: firstly preparing a melt spinning and medical silica gel tube composite mandrel, and then preparing the in-vivo engineering arterial blood vessel taking melt-spinning fiber as a skeleton by taking subcutaneous part of an animal as a bioreactor; the specific steps are as follows: 1) preparing the melt spinning and medical silica gel tube composite mandrel; 2) preparing the in-vivo engineering arterial blood vessel taking melt-spinning fiber as a skeleton by taking subcutaneous part of an animal as a bioreactor; and 3) performing in-situ transplanting of the in-vivo engineering arterial blood vessel so as to replace lesion blood vessels. The method has the advantages that the in-vivo engineering arterial blood vessel has good biocompatibility, all the components are originated from autologous tissues, and therefore, the artificial blood vessel is non-toxic, free from immunogenicity, and incapable of causing inflammation; due to the existence of melt spinning fiber, the prepared in-vivo engineering blood vessel has good mechanical strength, tenacity and compliance, and is suitable for surgical sewing operation.

The invention discloses a modeling device for establishing a bladder cancer orthotopic transplantation tumor animal model, solving the defects of long surgical time, complex operation, large animal bladder mucosa hurt and the like of the traditional modeling method. The modeling device comprises a needle seat fixedly arranged at the front end and a sleeve base provided with a cavity, wherein the front end of the sleeve base is fixedly provided with a soft sleeve communicated with the cavity, the front end of the needle base is sleeved and inserted in the cavity of the sleeve base and positioned by using a positioning baffle ring arranged on the needle base, a needle core on the needle base is sleeved and inserted in the soft sleeve of the sleeve base, and the length of the needle point ofthe needle core, exposed out of the end part of the soft sleeve, is 0.5-1.0mm, a break angle of 5-7 DEG is arranged at a place of the needle core, which is at a 1.5-2.0cm distance of the needle point, and a marking groove is arranged on the positioning baffle ring. The modeling device has the advantages of skilled design, simple structure and easiness in operation, greatly shortens the surgical time, and has little damage to the animal bladder mucosa and a tumor formation rate of reaching 100 percent.

The invention relates to a high metastasis human breast carcinoma cell subline MDA-MB-231sci, which has biological and pathological features and special metastasis phenotypes. The biological features are as follows: the MDA-MB-231sci cells are typical polygonal epithelioid cells, have rich cytoplasm and big and round karyon, grow adherent to the wall with almost uniform size and are closely arranged with clear boundary. The pathological features are as follows: a tumor cell of a nude mouse in vivo metastasis part has the similar structure with the subcutaneous tumor cell, short metastasis time and high metastasis rate, wherein the metastasis time is shortened to 26-42 days; a lung metastasis rate is 100%, meanwhile, a part of axillary lymph node metastasis of an animal is performed. An establishing method for the high metastasis human breast carcinoma cell line comprises the following steps of: (1) establishing a human breast carcinoma orthotopic transplantation tumor model; (2) sieving an orthotopic transplantation lung targeted metastasis model; and (3) establishing and passaging a human breast and lung carcinomas targeted high metastasis cell line. In the invention, the success rate is improved by sieving surgical excision tumor of each generation in vivo and corresponding breast.

The invention discloses a novel mouse in-situ pleural mesothelioma model and an establishment method thereof. The establishment method comprises the following steps of 1, taking 90 BLAB/C nude mice as an experimental group and 30 control groups, and uniformly putting the experimental group and the control groups into an incubator for normal culture; 2, under the condition that BLAB/C nude mice breathe, heartbeat and the like are normal, anesthetizing the mice and fixing the mice on an animal operating table in a supine position for experiment; and 3, at the intersection point of the left rib 5/6 intercostal space and the left chest wall anterior axillary line of the mouse, using microscopic forceps to lift and pull the skin at the intersection point, then using a 22G sterile sharp needle head to break the skin at the intersection point, then using a 30G sterile blunt pillow to break the wall layer pleura along the broken opening. The tumor transplantation method provided by the invention is applicable to orthotopic transplantation of pleural tumors. The growth mode, the invasion ability and the tumor metastasis effect of the pleural tumor can be restored to the maximum extent, and a good mouse model is constructed for studying the pleural tumor.

The invention discloses a preparation method of mouse ECMs (Extracellular Matrixes), obtained mouse ECMs and a mouse ovarium in-vivo regeneration method. The method comprises the following steps of 1, performing treatment for different time by using detergents with different concentrations, removing cells of mouse tissue organs, and preparing the ECMs; 2, performing histological analysis and biochemical analysis on the prepared ECMs so as to determine the optimum treatment condition; and 3, performing orthotopic transplantation on the ECMs subjected to cell removal treatment, recording the development condition of the ECMs and the ovarium regeneration efficiency, and further, verifying the regenerated ovarium tissues by a histology method, an immunohistochemistry method, a PCR (Polymerase Chain Reaction) method and the like. In addition, the effect that the tissue scaffolds (ECM) from different sources can also promote the ovarium regeneration is also proved. By using the method provided by the invention, the ovarium is successfully regenerated for the first time; and the further solving method is provided for the problem of female apogeny due to ovarium dysfunction. The method provided by the invention has the advantages of simplicity, convenience, low cost, low experiment condition requirements and high operability.

Described herein are methods and compositions for regulation of the p53 pathway, providing intrinsic “sternness” allowing for their efficient in vitro expansion following isolation. Pharmacologic approaches to modulate p53 signaling supports expansion of stem cells, including greater clonal expansion of lung stem cells when compared to use of other small molecules such as ROCK inhibitor Y27632 alone. Effects of combined treatment with Pifithrin-α and Y27632 are additive. The current invention involves use of drugs that target the p53 pathway to reversibly regulate stem cell expansion in vitro for banking of stem cells and for pre-conditioning of stem cells prior to orthotopic transplantation.

The invention discloses a traditional Chinese medicine composition for adjuvant treatment of a liver cancer. The traditional Chinese medicine composition is prepared from 10-20 parts of astragali radix, 5-15 parts of curcuma zedoary, 10-30 parts of manyleaf paris rhizome, 10-20 parts of hairyvein agrimony, 5-10 parts of radix asparagi, 5-10 parts of coix seeds, 15-30 parts of ardisiae japonica, 15-30 parts of oldenlandia diffusa, 15-30 parts of rhizoma pinelliae and 15-30 parts of chinaroot greenbrier, 10-40 parts of ganoderma lucidum and 15-30 parts of Chinese actinidia roots. It is shown according to experimental results that the traditional Chinese medicine composition combined with the chemotherapeutic drug cisplatin can obviously inhibit the growth and metastasis of liver cancer orthotopic transplanted tumors, can reduce the toxic and side effects of the chemotherapeutic drug, prevent the damage of the cisplatin to immune organs and improve the inhibition effect of the cisplatin on bone marrow. The traditional Chinese medicine composition combined with the chemotherapeutic drug cisplatin induces apoptosis of the human hepatoma cells HepG2, which indicates that the combined useof the traditional Chinese medicine composition and the chemotherapeutic drug cisplatin can produce effect-enhancing and toxicity-reducing action, and the traditional Chinese medicine can be used asan adjuvant radio therapy and chemotherapy medicine for clinically treating tumors.

The invention discloses a method for building a triple negative breast cancer living tumor tissue mouse orthotopic transplantation model. The method comprises the following steps of (1) subcutaneouslyembedding an estradiol sustained-release tablet in the neck of an immunodeficient mouse; (2) selecting triple negative breast cancer living tumor tissues of a clinical triple negative breast cancer patient through an operation, and then cutting the triple negative breast cancer living tumor tissues into inoculation tumor blocks with the volume being 4 to 15 mm<3>; (3) performing orthotopic transplantation on the inoculation tumor blocks into a mammary gland fat pad of the immunodeficient mouse; and (4) raising the immunodeficient mouse for 30 to 80 days, and building the triple negative breast cancer living tumor tissue mouse orthotopic transplantation model. The method has the advantages that the estrogen level in a female mouse model body can be improved; and the growth requirement of the triple negative breast cancer tissues is met, so that the tumor nodule emergence time is early, the model building time is shortened, the tumor forming rate is improved by nearly 4 times, and the model building method is simple and feasible.

The invention relates to the technical field of medicines, and particularly discloses application of berberine hydrochloride in restraining the pancreatic cancer. After the interference of the berberine hydrochloride, the growth speed of the pancreatic cancer orthotopic transplantation tumor is obviously reduced, and the tumor size is obviously reduced; meanwhile, the berberine hydrochloride has the remarkable restraining effect on the pancreatic cancer cell proliferation. Specially, we find that the berberine hydrochloride has the good restraining effect on the pancreatic cancer under the lowdosage. In addition, the berberine hydrochloride is rich in source, low in price and high in safety, and therefore the cost for treating the pancreatic cancer can be reduced and the toxic and side effects caused by treatment can be reduced by using the berberine hydrochloride as the pancreatic cancer treatment means.