Construction method of malignant neurinoma xenograft mouse model

A mouse model and xenotransplantation technology, which is applied in the field of establishment of tumor transplantation mouse models, can solve the problems of high failure rate in clinical trials of anti-tumor drugs, achieve the effects of preventing medical social burden, maintaining consistency, and reducing drug side effects

Active Publication Date: 2019-12-31
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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Problems solved by technology

Studies have found that cells continuously adapt to the in vitro environment during the passage process, and their biological characteristics are inconsistent with tumor c

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  • Construction method of malignant neurinoma xenograft mouse model
  • Construction method of malignant neurinoma xenograft mouse model
  • Construction method of malignant neurinoma xenograft mouse model

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Embodiment

[0038] Subcutaneous inoculation: select 10 4-week-old NOD / SCID mice. NOD / SCID mice are non-obese diabetic / severe combined immunodeficiency mice. With a body weight of about 12-15g, soak fresh tumor tissues (or tumor tissues from patients with malignant schwannoma after cryopreservation and resuscitation in liquid nitrogen) in a sterile incubation solution of pure FBS+penicillin and streptomycin (the final concentration of penicillin is 100U / ml, the final concentration of streptomycin is 100U / ml), cut into 1*1*1mm small pieces with sterile scissors. After the mice were anesthetized and fixed, a skin incision was made near the right armpit, the tumor tissue block was implanted subcutaneously (P0), and the surgical incision was sutured. The mice were observed for tumor growth every day. When the tumor grows to a diameter of more than 5mm. Measure the tumor twice a week with a vernier caliper and record the length (a) and width (b) of the tumor, and calculate the tumor volume (V...

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Abstract

The invention provides a construction method of a malignant neurinoma xenograft mouse model. The method comprises the following steps of: resuscitating a tumor tissue block of a malignant neurinoma patient stored in a liquid nitrogen environment; and carrying out xenogeneic subcutaneous transplantation on the resuscitated tumor tissue block of the patient into immunodeficient mouse bodies under asterile condition. The xenogeneic subcutaneous transplantation comprises the following steps: selecting immunodeficient mice with the age of 2-6 weeks, wherein the sex of the mice is kept consistent with that of the patient; soaking the resuscitated tumor tissue block of the patient in sterile incubation liquid containing fetal calf serum; fixing the mice after anesthesia; implanting the tumor tissue block of the patient under the skin of the mice; suturing incision; and raising the mice to enable tumors in the body of the mice to grow. The method also includes: performing xenogeneic orthotopic transplantation on the cryopreserved and resuscitated tumor tissue block of the patient into immunodeficient mice. According to the invention, the xenograft mice are used for replacing clinical patients to screen different combined medicines, so that the most appropriate medicine is selected, the effectiveness is greatly improved, and the side effects of the medicines are reduced.

Description

technical field [0001] The invention relates to the field of establishment of tumor transplantation mouse models, in particular to a construction method of malignant schwannoma xenograft mouse models. Background technique [0002] Malignant schwannomas, also known as neurosarcomas, originate from the peripheral nerve sheath and are composed of malignant neuroblasts, most of which are malignant transformations of neurofibromas or schwannomas. It is highly malignant, prone to lung metastasis, extremely high morbidity and mortality, and poor prognosis. Malignant schwannomas have brought a heavy economic burden to the society and even the country, and brought about greater social problems. Because of the heterogeneity of malignant schwannoma, previous basic research results based on malignant schwannoma cell lines can rarely be reproduced in patients with clinical malignant schwannoma. The level of diagnosis and treatment of schwannomas lags behind. [0003] Invention patent ...

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Application Information

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IPC IPC(8): A61D1/00
CPCA61D1/00
Inventor 黎志宏万璐刘忠越段智曦张程豪罗煦君
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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