Building method of human choriocarcinoma naked mouse orthotopic transplantation tumor model

Abstract

The invention relates to the technical field of biology, in particular to a building method of a human choriocarcinoma naked mouse orthotopic transplantation tumor model. The building method includessteps: S1, culturing human choriocarcinoma JAR cells; S2, selecting a female naked mouse, and injecting 200ul of a human choriocarcinoma JAR cell suspension to a right-side inguinal position; removingtumor tissue when tumor grows to 2cm3 in size; S3, in a sterile condition, using sterile normal saline to rinse choriocarcinoma tissue, cutting into tissue blocks of 1mm3, and putting into the sterile normal saline for standby use; selecting a female naked mouse, performing general anesthesia, transplanting the choriocarcinoma tissue blocks into corporis uteri cavum, suturing layer by layer, andclosing abdomen; continuing to feed after operation, and allowing free intake, in this way, the human choriocarcinoma naked mouse orthotopic transplantation tumor model is built in the body of the naked mouse. By simulating tumor cell growth microenvironment and conforming to clinical genesis and development of tumor, a good foundation is laid for further studying pathogenesis and clinical treatment of human choriocarcinoma.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a method for constructing a human choriocarcinoma nude mouse orthotopic transplantation tumor model. Background technique [0002] Choriocarcinoma, referred to as choriocarcinoma, is a malignant trophoblastic tumor secondary to normal or abnormal pregnancy, often secondary to hydatidiform mole, miscarriage or full-term delivery. A few can occur after ectopic pregnancy, mostly women of childbearing age. Ovarian cancer that occasionally occurs in unmarried women is called primary choriocarcinoma. Choriocarcinoma is highly invasive and is a highly aggressive disease. The most common distant metastatic sites are the lung, brain, and liver. The current treatment strategies for choriocarcinoma mainly adopt chemotherapy and surgical resection, but the response to chemotherapy is large, and some patients still die due to tolerance or unacceptable side effects of chemotherapy. In r...

AI Technical Summary

Problems solved by technology

[0003] Although subcutaneous xenograft tumors in nude mice are currently the most commonly used animal model in the study of choriocarcinoma, unfortunately, the clinical progress of subcutaneous xenograft tumors and choriocarcinoma is somewhat different, and the biology of human choriocarcinoma cannot be completely preserved. characteristic

[0004] At present, there are no reports on the construction of orthotopic transplanted tumor models of choriocarcinoma in nude mice at home and abroad, and there is no nude mouse model that can replicate the progress of choriocarcinoma

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