Method for establishing green fluorescence BALB/c nude mouse model and model application

Abstract

The invention discloses a method for establishing a green fluorescence BALB/c nude mouse model. The method includes: selecting female C57BL/6-Tg (CAG-EGFP) mice and male Foxn1nu mice to perform hybrid to acquire F1 child generation hairy fluorescence mice, selecting the female mice, selecting the female mice having sexual maturation and the parental generation male Foxn1nu mice to perform mating (backcross) to acquire an F2 child generation; generating segregation of characters of the F2 child generation, selecting female hairy fluorescence mice and the parental generation male Foxn1nu mice to perform backcross, and completing a backcross cycle; performing the backcross cycle for ten times to acquire an F10 child generation, and establishing the green fluorescence BALB/c nude mouse model. The invention also discloses application of an animal model established by the method to a brain tumor transplanting model. The method uses a homogenous introduction method to establish the new strain model mice which can stably express EGFP, and can lay a firm foundation for human glioma orthotopic transplantation tracking and research on glioma and a micro environment thereof.

Description

technical field [0001] The invention belongs to the field of animal models used in the medical field, in particular to mouse models. Background technique [0002] Glioma is the most common primary refractory brain tumor in the brain. At present, domestic and foreign laboratories are conducting extensive basic research on glioma, including the mechanism of glioma, biological behavior, gene pathways and Biological therapy and gene therapy, etc., have improved the curative effect and improved prognosis to a certain extent. As a platform for basic research, it is crucial to establish an orthotopic transplantation model of human glioma. Although the research on such animal models is relatively mature, there are certain defects: the tumor model established with ordinary BALB / c nude mice cannot track and distinguish the tumor and the tumor microenvironment cells derived from the host; c The fluorescent nude mice crossed with fluorescent mice with normal immune function, because o...

AI Technical Summary

Problems solved by technology

Although the research on such animal models is relatively mature, there are certain defects: the tumor model established with ordinary BALB / c nude mice cannot track and distinguish the tumor and the tumor microenvironment cells derived from the host; The fluorescent nude mice crossed between nude mice and fluorescent mice with normal immune function, because of their unstable genetic traits, the fluorescent expression of their offspring is not stable

[0004] At present, transgenic green fluorescent mice can emit green fluorescence because of their whole body tissues and organs, so they are suitable for studying the interaction between exogenous tissues or cells (usually another fluorescent marker) and host (mouse) tissue cells in the mouse. In vivo research, but the mouse cannot be transplanted with human tissues or cells and survive due to its sound immune function, so it cannot be used for in vivo fluorescent tracer experiments on human tissue cells

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