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Application of berberine hydrochloride in restraining pancreatic cancer

A kind of technology of berberine hydrochloride and pancreatic cancer

Inactive Publication Date: 2019-05-10
FUDAN UNIV SHANGHAI CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern pharmacological studies have found that berberine hydrochloride has various anti-tumor effects, and has inhibitory effects on liver cancer, lung cancer, prostate cancer, ovarian cancer, leukemia, etc., but because berberine hydrochloride is a quaternary ammonium salt structure, it has poor lipophilicity, It is difficult to reach the effective required concentration in vivo through the lipid membrane of cells, and the anti-tumor effect of berberine hydrochloride in vivo is not as obvious as that in vitro, which makes the application of berberine hydrochloride in the treatment of chronic diseases very challenging. There are reports on the in vivo experimental results of berberine hydrochloride inhibiting pancreatic cancer

Method used

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  • Application of berberine hydrochloride in restraining pancreatic cancer
  • Application of berberine hydrochloride in restraining pancreatic cancer
  • Application of berberine hydrochloride in restraining pancreatic cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] In this example, the effect of berberine hydrochloride on orthotopic transplanted tumors of pancreatic cancer was observed through immunosuppressive nude mouse pancreatic orthotopic transplanted tumor experiments.

[0038] 1. Preparation of pancreatic cancer mouse model

[0039] Experimental animals:

[0040]SPF-level immunosuppressive nude mice (BALB / cAnN-nu(Nude)), aged 4-6 weeks, female, weighing 18-20g, were purchased from the Laboratory Animal Unit of the University of Hong Kong and kept in an independent ventilation system M2 mouse cage. Animal Use and Ethics License CULATR No. 4077-16 was obtained from the Committee on the Use of Liver Animals in Teaching & Research, The University of Hong Kong. The experiment operator obtained the animal license (Animal Lisence) of the Hong Kong Special Administrative Region Government, No.: (16-634) in DH / HA&P / 8 / 2 / 3Pt.88. All animal experiments were performed in accordance with the Guidelines for the Care and Use of Laborato...

Embodiment 2

[0078] In order to better simulate the situation in the human body, this embodiment selects SPF level mouse C57BL / 6 (female, 4-6 weeks old) to establish a pancreatic cancer model, and inoculates mouse pancreatic cancer cells Panc02-Luciferase 1×10 6 Individual / mouse, to evaluate the effect of berberine hydrochloride on the growth of orthotopic transplanted tumors of pancreatic cancer.

[0079] 1. Preparation of pancreatic cancer mouse model

[0080] Specific experimental methods:

[0081] Press Matrigel with 1 x 10 8 The volume ratio of Panc02-Luciferase cells per milliliter was 1:1 to configure the cell suspension in situ, and then the cell suspension was injected into the pancreas of mice in situ. The modality for laparotomy for orthotopic implantation of pancreatic tumors is described below:

[0082] (1) The mice were weighed and anesthetized, and each mouse was injected intraperitoneally with 0.2 mL of a ketamine / xylazine mixture, wherein the doses of ketamine and xylaz...

Embodiment 3

[0102] In this example, through in vitro experiments, the inhibitory effect of berberine hydrochloride on the proliferation and migration of pancreatic cancer cell lines was observed.

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PUM

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Abstract

The invention relates to the technical field of medicines, and particularly discloses application of berberine hydrochloride in restraining the pancreatic cancer. After the interference of the berberine hydrochloride, the growth speed of the pancreatic cancer orthotopic transplantation tumor is obviously reduced, and the tumor size is obviously reduced; meanwhile, the berberine hydrochloride has the remarkable restraining effect on the pancreatic cancer cell proliferation. Specially, we find that the berberine hydrochloride has the good restraining effect on the pancreatic cancer under the lowdosage. In addition, the berberine hydrochloride is rich in source, low in price and high in safety, and therefore the cost for treating the pancreatic cancer can be reduced and the toxic and side effects caused by treatment can be reduced by using the berberine hydrochloride as the pancreatic cancer treatment means.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of berberine hydrochloride in inhibiting pancreatic cancer. Background technique [0002] Pancreatic cancer is a common clinical malignant tumor with high malignancy, high lethality, and poor prognosis. Because of its insidious onset, most patients are already in the middle and advanced stages when they are diagnosed. At present, the 5-year survival period of pancreatic cancer is only about 5%, and the mortality rate in the world is very high. The mortality rate in China is 90.8%, the mortality rate in the United States is 78.5%, and the mortality rate in Canada is 95.0%. According to the epidemiological survey in China, pancreatic cancer is the seventh deadliest tumor disease in China. At present, in addition to surgical resection, radiotherapy and chemotherapy can only prolong the survival of patients with pancreatic cancer to a limited extent, and only 10-15%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4375A61P35/00
Inventor 程健珊陈震冯奕斌王宁陈联誉
Owner FUDAN UNIV SHANGHAI CANCER CENT
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