Method for building improved orthotopic transplantation tumor model of colorectal tumor in nude mice

A method for constructing transplanted tumors, which is applied in the field of colon cancer transplanted tumor models, can solve the problems of high mortality of experimental animals, gastrointestinal obstruction, and death of experimental animals, and achieve high modeling success rate, prolonged survival time, and simple method Effect

Inactive Publication Date: 2010-12-22
LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But in our research process, found some shortcomings of this model: the tumor grows too fast, easily forms the obstruction of d

Method used

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  • Method for building improved orthotopic transplantation tumor model of colorectal tumor in nude mice
  • Method for building improved orthotopic transplantation tumor model of colorectal tumor in nude mice
  • Method for building improved orthotopic transplantation tumor model of colorectal tumor in nude mice

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Embodiment 1

[0021] 1. Materials and Methods

[0022] 1.1 Cell lines and reagents Digestive solution (0.25% trypsin solution + 1mM EDTA), RPMI1640 + double antibody culture medium containing 10% fetal bovine serum, PBS solution, CO2 incubator, inverted microscope, ethyl succinate erythromycin tablets

[0023] 1.2 Animals 20 BALB / C nude mice, SPF grade, purchased from the Chinese Academy of Sciences, aged 6-8 weeks, weighing 18-20 g. They were randomly divided into 10 rats in the control group and 10 rats in the model group.

[0024] 2. Construction of animal models

[0025] Build process such as figure 1 shown.

[0026] 2.1 Preparation of tumor source

[0027] Human colon cancer cells SW480 were cultured and subcultured, and the tumor cells in the logarithmic growth phase were used to prepare a single cell suspension, which was injected subcutaneously into the right armpit of nude mice to prepare the tumor source. The specific method is the same as that described in the summary of th...

Embodiment 2

[0036] 1. Materials and Methods

[0037] 1.1 Cell lines and reagents Digestive solution (0.25% trypsin solution + 1mM EDTA), RPMI1640 + double antibody culture medium containing 10% fetal bovine serum, PBS solution, CO2 incubator, inverted microscope, ethyl succinate erythromycin tablets

[0038] 1.2 Animals 20 BALB / C nude mice, SPF grade, purchased from the Chinese Academy of Sciences, aged 6-8 weeks, weighing 18-20 g. They were randomly divided into 10 rats in the control group and 10 rats in the model group.

[0039] 2. Construction of animal models

[0040] Build process such as figure 1 shown.

[0041] 2.1 Preparation of tumor source Human colon cancer cells LOVO were cultured and passaged, and the tumor cells in the logarithmic growth phase were used to prepare a single-cell suspension, which was injected subcutaneously into the right armpit of nude mice to prepare the tumor source. The specific method is the same as that described in the summary of the invention. ...

Embodiment 3

[0050] 1. Materials and Methods

[0051] 1.1 Cell lines and reagents Digestive solution (0.25% trypsin solution + 1mM EDTA), RPMI1640 + double antibody culture medium containing 10% fetal bovine serum, PBS solution, CO2 incubator, inverted microscope, ethyl succinate erythromycin tablets

[0052] 1.2 Animals 20 BALB / C nude mice, SPF grade, purchased from the Chinese Academy of Sciences, aged 6-8 weeks, weighing 18-20 g. They were randomly divided into 10 rats in the control group and 10 rats in the model group.

[0053] 2. Construction of animal models

[0054] Build process such as figure 1 shown.

[0055] 2.1 Preparation of tumor source Human gastric cancer cell MKN-45 was cultured and passaged, and the tumor cells in the logarithmic growth phase were used to prepare a single cell suspension, which was injected subcutaneously into the right armpit of nude mice to prepare the tumor source. The specific method is the same as that described in the summary of the invention....

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Abstract

The invention discloses a transplantation tumor model of colorectal tumor for medical scientific research, which is produced by improving the orthotopic transplantation tumor model of colorectal tumor in nude mice. The invention aims to overcome the defect that the orthotopic transplantation tumor model of colorectal tumor has high intestinal obstruction incidence rate and short model animal survival time and is not suitable for long-time experiments. In the invention, the prepared tumor is transplanted close to the blood vessel in mesocolon. The method comprises the following steps: (1) preparation of tumor: injecting single cell suspension of the tumor cell to subcutis of nude mice and preparing the source of the transplantation tumor; (2) transplanting the prepared tumor close to the blood vessel in mesenterium of nude mice but not the serosa of the intestinal tube; and (3) raising the nude mice in an aseptic laminar flow room, controlling the room temperature to be (25+/-1) DEG C and relative humidity to be 40-50% and continuously observing the nude mice. The animal model built by the invention can reproduce the natural clinical pathology process of human colorectal tumor, can not suffer from obstruction of the lumen in the digestive tract in the short term and has transfer mode similar to that of the patients.

Description

technical field [0001] The invention relates to a new transplanted tumor model of colon cancer for medical scientific research, which is improved on the basis of the orthotopic transplanted tumor model of colon cancer in nude mice. Background technique [0002] At present, animal models of intestinal tumors at home and abroad include: chemical carcinogen induction method, tumor transplantation method and transgenic animal models. [0003] The chemical carcinogen induction method takes a long time to build a model and has a low success rate, so it is rarely used at present. [0004] The cost of transgenic mice is high, and there are few strains, so it is difficult to apply them in long-term large-scale experiments. [0005] Tumor transplantation is currently the most commonly used method. Its advantages are: a group of animals can be inoculated with the same amount of tumor cells at the same time, the growth rate is relatively consistent, individual differences are small, t...

Claims

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Application Information

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IPC IPC(8): A61D7/00
Inventor 张静喆杨海波章学林蔡骏宋华荣梁晓强
Owner LONGHUA HOSPITAL SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE
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