61results about How to "High modeling success rate" patented technology

The invention relates to a preparation method of a 2-type diabetic nephropathy model. The method comprises the following steps of: selecting a male SHR (spontaneously hypertensive rat) rat with an age of 6 weeks, wherein the blood pressure of the male SHR rat at birth is normal, and at the time of 4 weeks, the blood pressure begins to rise; after adaptively feeding the SHR rat selected in the step (1) for one week, feeding a high sugar and high fat feed for 2 weeks; detecting the weight, the blood fat, the blood sugar and the insulin concentration and calculating an insulin resistance index evaluated by a steady-state model, wherein the insulin resistance index is not smaller than 3.8, and the high fat and high sugar feed comprises the following components in percentage by weight: 10.0 percent of lard, 20.0 percent of cane sugar, 2.5 percent of cholesterol, 2.0 percent of cholate and 66.5 percent of conventional feed; after fasting the insulin resistant SHR rat for 12 hours, carrying out a small-dose one-time STZ (streptozocin) (35mg/kg) intraperitoneal injection on the SHR rat, wherein the small dose is 35mg/kg; after 72 hours, measuring the random blood sugar; and judging the 2-type diabetic nephropathy model to be successfully prepared if the plasma glucose is not smaller than 16.7mmol/L.

The invention discloses a new method for screening an anti-liver injury medicament by using a model organism zebra fish. The method comprises the following steps: continuously molding the zebra fish serving as a molding object for 3 days by using preferred carbon tetrachloride at the concentration of 1mmol/L to obtain the zebra fish of a liver injury model, and then evaluating the anti-liver injury activity of different receptor medicaments by using ALT and AST levels in the liver tissue of the zebra fish and the pathological change condition of the liver tissue of the zebra fish. Experimental results show that the new method for screening the anti-liver injury medicament by using the model organism zebra fish has high molding success rate, can simulate an in vivo environment to perform quick screening, and has the advantages of strong operability, accurate experimental results, little amount of required reacceptance medicament, strong repeatability, low cost, wide application range, capability of performing batch screening experiments and high work efficiency.

The invention discloses a zebra fish vascular injury model for screening a vascular injury resisting medicament as well as a building method and application thereof. According to the invention, a fertilized zebra fish embryo is treated with a vascular growth factor receptor inhibitor to obtain zebra fish suffering from vascular injury so as to build the zebra fish vascular injury model, then the zebra fish is treated with a detected medicament for 1-3 days, and the gene expression level of the vascular endothelial growth factor receptors (Flk-1A, Flk1-B and Flt1) in the zebra fish tissues is detected by morphological observation and real-time quantitative PCR (polymerase chain reaction) so as to judge the treating effect of the detected medicament on the zebra fish vascular injury. Experiment results prove that the zebra fish vascular injury model built according to the method provided by the invention has extremely high model forming rate, and can simulate in-vivo insufficient angiogenesis environment to quickly screen medicaments for promoting angiogenesis; the effect is easy to observe and is obvious; the medicament dosage in the experiment process is low; and the experiment results are accurate and strong in repeatability. The model can be used for screening potential medicaments in batch with high working efficiency.

The invention discloses an entity relationship-based feature construction method, and the method comprises the steps: obtaining a main table in a relational database and a plurality of auxiliary tables associated with the main table, enabling the main table to be provided with a main key column and a plurality of external key columns, enabling each entry in the main table to correspond to one entity, and enabling the auxiliary tables to be associated with the main table through external keys of the main table; constructing a directed inter-table relational graph by taking the main table and the auxiliary table as nodes and taking the incidence relation between every two of the main table and the auxiliary table as an edge; taking a node corresponding to the main table as a starting point,and traversing the inter-table relation graph to acquire inter-table relation data of each entity in the main table and the corresponding auxiliary table; and performing conversion calculation on theinter-table relationship data based on a preset conversion function to construct features corresponding to each entity in the main table. The invention further discloses a feature construction deviceand equipment based on the entity relationship and a storage medium. According to the method, the feature data is acquired based on the inter-table relational graph, and the features of the data can be represented from multiple dimensions on the whole, so that the modeling success rate is improved.

The invention provides a culture and cryopreservation method for a prostatic cancer organoid with a tumor immune microenvironment. The culture and cryopreservation method comprises the following steps: separating mononuclear cells and autoserum from peripheral blood, and stimulating the mononuclear cells by using a CD28 antibody and IL-2; separating tumor cells from a prostatic cancer tissue sample; mixing the tumor cells with a culture medium and a temperature-sensitive hydrogel, and then carrying out culturing and subculturing to obtain a tumor organoid precursor; stimulating the tumor organoid precursor by using IFN-gamma, and then conducting dissociating to form stimulated tumor cells; mixing the stimulated tumor cells with the stimulated mononuclear cells, conducting stimulating witha PD-1 antibody, mixing the stimulated mixture with the temperature-sensitive hydrogel, and carrying out culturing; and cryopreserving the tumor organoid with a cryopreservation solution containing FBS and the temperature-sensitive hydrogel. With the method in the invention, the success rate of culturing of organoids with a tumor microenvironment can be greatly improved at low cost.

The invention provides an establishment method of an endoplasmic reticulum stress induced mouse acute liver injury model. The establishment method orderly comprises the following steps: carrying out intragastric administration treatment on mice by use of an endoplasmic reticulum stress inducer with a dosage of 0.2ml/10g for each mouse, and completing the establishment of the mouse acute liver injury model in 8-48 hours after administration, wherein the endoplasmic reticulum stress inducer is prepared by a method comprising the steps of taking (0.5-4)mg of tunicamycin for dissolving 100 microliters of dimethyl sulfoxide to obtain a preservation solution, taking 100 microliters of preservation solution and adding 19.9ml of double distilled water to 20ml, and thus completing the preparation of the endoplasmic reticulum stress inducer. The mouse model of the induced acute liver injury is established by inducing the endoplasmic reticulum stress effect; through the study on the mechanism of action of the endoplasmic reticulum stress, the self regulation ability of cells in the stress state can be further known, and the occurrence mechanism of the liver diseases can be further known; therefore, new intervention and treatment measures can be adopted for the liver diseases to achieve the purposes of preventing and treating diseases.

The invention discloses a method for making a dry age-related macular degeneration disease model on a primate, namely applying sodium iodate onto the primate with a carotid artery injection method. The method is simple and easy, high in success rate and good in repeatability. The model made with the method is stable. The amount of sodium iodate needed is small, injury of large doses of sodium iodate to the whole body of the animal is avoided, and the animal can live for a long time so that follow-up tests can be made possible. A model of any one side or a model of double sides can be made as needed. When the one-side model is made, a lateral eye can not be hurt at all, and normal living vision of the animal is guaranteed. Comparison of two lateral eyes of one animal can be conducted to provide credibility for the test, and the number of animals needed for the test is reduced. The method provides a sound tool for the dry age-related macular degeneration research and has great significance for the pathological physiology research and treatment research of the disease.

The invention discloses a culture method of 3D tumor organs for anti-tumor drug sensitivity test of gastric cancer to solve the problem of low success rate of modeling of existing tumor organs. The method comprises the following specific steps: Step 1, preparation before an experiment; Step 2, tissue sampling; Step 3, tissue enzymatic separation; Step 4, PDO tumor organ modeling; and Step 5, PDO culture. The design of the method is reasonable. By adding gastrin during the culture process, the modeling success rate and cell viability of gastric cancer organs are promoted. It is found through comparison that when the gastrin-added medium is used to culture gastric cancer PDO, the formed PDO mass increases significantly, the cell viability is also increased, and the modeled tumor formation rate is increased significantly to 80%. It shows that the use effect is good and the method can provide a basis for the test of tumor drug sensitivity, screening of anti-tumor drugs for patients and clinical medication.

The invention discloses a novel casting mold and a molding method thereof. The casting mold consists of a rotary disk (1), a base plate (2), a fixed scraping plate (3), a steel outer mold (4), steel upper mold (5), fixed shafts (6), (7) and (9) and a bracket (8). The molding method comprises the following steps of: placing a prefabricated two-sheet mold sample onto the edge of the base plate (2); matching the steel outer mold (4) and fastening; filling sand into the steel outer mold (4) and ramming; striking off a top surface with the scraping plate when the sand reaches the top surface; putting a top plate mold sample; matching the steel upper mold (5); filling sand into the steel upper mold (5) and ramming; leaving a sprue gate and an air outlet hole; opening the steel upper mold (5) and the steel outer mold (4); taking the mold sample out; and starting casting. When a second casting is required to be cast, the method comprises the following steps of: enclosing the two-sheet mold sample; filling sand into the cavity of the two-sheet mold sample; ramming; taking the mold sample away; scraping a mold cavity by rotating the rotary disk (1) and using the fixed scraping plate (3); and matching the steel outer mold (4) and the steel upper mold (5) to cast. The novel casting mold has the advantages of saved labor force, low labor intensity, high success rate, high accuracy, easiness for demolding, short flow, low time consumption and high efficiency, and is particularly suitable for casting container castings such as copper drums, boilers, basins and the like.

The invention discloses an establishing method of a rat atrial fibrillation model for simulating characteristics of human diseases. The establishing method comprises the steps that 1) an experimentalrat is raised to 35-45 weeks; 2) for the experimental rat, overload of the rat heart pressure is induced by cervical transverse aortic constriction, and a TAC model rat is formed; 3) after the transverse aortic constriction is carried out on the TAC model rat for 1-2 weeks, small animal ultrasonic treatment is conducted to detect a two-dimensional ultrasonic image and color Doppler flow image of blood vessels of a constriction part; 4) after the TAC model rat is constructed for 6-9 weeks, the inner diameter of the left atrium is detected through small animal ultrasonic treatment, the myocardial metabolism is detected by adopting PET, and the occurrence of atrial remodeling is verified; 5) after the transverse aortic constriction is carried out on the TAC model rat for 6-9 weeks, atrial fibrillation is induced through esophageal electrostimulation, a bioelectrical signal recorder is used for recording an electrocardiogram of the experimental rat body surface, and the rat atrial fibrillation model is obtained through verification. The constructed rat atrial fibrillation model can reflect the characteristics of the clinical human diseases, and the establishing method is simpler, moreconvenient, more economical and more efficient.

The invention belongs to the technical field of testing or analyzing materials by means of chemical or physical properties of a measuring material, and discloses a method for measuring the regulationand control of Chemerin on insulin resistance and the intervention of a CMKLR1 agonist, which comprises the following steps: preparing a pancreatic diabetes model by adopting an arginine induction method, performing intraperitoneal injection of 350 mg/kg arginine once a day for 6 weeks, and establishing a pancreatic diabetes group by a mouse common diet; during the period, establishing a pancreas-derived diabetes mellitus group by the common diet of the mice;meanwhile, establishing a high-fat diet group by adopting a formula feed open diet; establishinga negative control group, wherein the mice are fed with ordinary diet and water for 6 weeks; adopting a general pathology, an enzyme-linked immunosorbent assay, a Real-Time PCR method and an intraperitoneal injection glucose tolerance test for detection; expressing all the detection data by mean plus or minus standard deviation, and testing the normal distribution is by Shapiro Wilk test; using single-factor variance analysis to comparegroups with each other, and if the groups do not conform to the normal distribution, using Mann-Whitney U test for comparison; significant difference lying in P < 0.05; applying SPSS 22.0 for Windowssoftware to implement the entire verification process.

The present invention discloses a feed for rat obese models. The feed comprises the following components in parts by weight: 25-30 parts of corn, 15-20 parts of wheat, 20-25 parts of soybean meal, 5-12 parts of wheat gluten proteins, 3-10 parts of fishmeal, 10-25 parts of lard, 2.9-9 parts of yeast hydrolyzate, 0.8-1.2 parts of stone powder, 1-2 parts of calcium hydrogen phosphate, 0.5-1 part of compound mineral, 0.1-0.5 part of compound vitamin, 0.1-0.2 part of methionine and 0.1-0.2 part of lysine. The feed is reasonable in fat content, can improve a success rate of the rat obese models, contains the wheat gluten proteins most suitable for absorption of rats, promotes absorption of proteins by the rats, enables the rats to be high in survival rate, is reasonable in component ratios, has synergistic effects, and can significantly improve the success rate of the rat obese models. Nutritional indicators of the feed are line with requirements of the nutritional indicators in national standard GB14924.3-2010 of "Experimental Animal Full Price Nutritional Feeds".

The present invention relates to a new medicine application of elastic proteinase inhibitor, in the concrete, it relates to an application of neutral granulocyte elastic proteinase inhibitor sevilesta in preparation of medicine for preventing or/and curing cerebral hemorrhage. It can be freeze-dried powder injection and injection liquor.

The invention discloses a medicinal composition for establishing a hyperuricemia animal model and an application thereof. The medicinal composition comprises ethambutol and uric acid, and can be used for preparing an acute hyperuricemia model with short modeling time, stable model and high modeling success rate. The medicinal composition is administrated to a rhesus monkey in an intragastric manner, a stable and reliable acute hyperuricemia model can be quickly obtained, and the modeling method is reasonable and close to the pathogenesis of human beings.

The invention belongs to the technical field of animal disease models, and specifically discloses an improved SD (Sprague Dawley) rat CD (Crohn's Disease) modeling method. The improved SD rat CD modeling method comprises the following steps: after fasting and narcotizing an SD rat, carrying out clysis by using a modeling drug (which is prepared by mixing 5 percent of a TNBS (Trinitro-Benzene-Sulfonic Acid) water solution with absolute ethyl alcohol according to a volume ratio of 1 to 1 and is 0.2 ml/100 g in body mass) according to the dosage of 50 mg/kg, adopting a body position of enabling the head of the SD rat to face downwards and the tail to face upwards after leading in the modeling drug so as to prevent clysis liquid from flowing outwards, and reversely carrying the SD rat for 1 to 2 minutes. The improved SD rat CD modeling method disclosed by the invention is simple and convenient in operation and good in repeatability, and a model accords with the features such as diarrhea, bloody stools, intestinal obstruction, weight lose, colonic ulcers and adhesion and abscess between intestinal canals and between an intestinal canal and visceral organs or tissues of clinic human CD; the modeling success rate is high, and the animal death rate is low.

The invention provides a breast cancer bone metastasis laboratory mouse disease model building method. The method comprises the following steps: selecting a mouse, anesthetizing the mouse, removing hair on the outer side of the thigh of the mouse, disinfecting the removed area, cutting open the skin of the mouse along the longitudinal axis of the femur, bluntly separating subcutaneous fascia, exposing the distal end of the femur, breaking the continuity of the femur of the mouse to form incomplete fracture, injecting tumor cells into the fracture part of the mouse, quickly closing the intermuscular space, washing the skin operation area of the mouse, injecting energy liquid into the abdominal cavity, and placing the mouse in a resuscitation box. According to the breast cancer bone metastasis laboratory mouse disease model building method provided by the invention, the tumor formation time is shortened by simulating bone tumor invasion in a bone dissolving state in a discontinuous fracture environment, and a bone marrow immune environment can be destroyed, so that immune escape of the tumor cells is realized, the attack of an immune system is avoided, tumors are formed, the survival rate of the tumors is increased, and the modeling success rate is increased.

The invention relates to a method for inducing a non-alcoholic fatty liver disease model through fluorine intervention. The method comprises the steps that high-fat diet is combined with fluorine intervention to induce the non-alcoholic fatty liver disease of an animal, the fat content in the high-fat feed is 45-60%, 0.01%-0.2% of sodium fluoride is added into the high-fat feed, or 50-1000 mg/L of sodium fluoride is added into drinking water of the animal, and the intervention period is 2-3 months. The non-alcoholic fatty liver disease animal model is high in modeling rate, stable in performance, low in cost and short in period, and the non-alcoholic fatty liver disease can be systematically and scientifically researched by utilizing the method.

The invention relates to a method for establishing a spinal dura mater leakage arachnoid hernia type sacral canal cyst model. The method comprises the following steps: step 1) selecting a model animal; 2) exposing the tail end of the dural sac in the lumbosacral vertebrae of the model animal through an operation; 3) irregularly cutting the tail end of dural sac or thick nerve sleeve adventitia; and 4) completing modeling. By means of the method, the spinal dura mater leakage arachnoid hernia type sacral canal cyst animal model with the survival rate of 100%and the modeling success rate of 60%can be simulated and the cyst form is very similar to that in real cases.

The invention provides a preparation method of a non-alcoholic fatty liver disease cell model induction liquid and application of the induction liquid and belongs to the technical field of biologicalmodel establishment. The induction liquid comprises a degreased bovine serum albumin solution of stearic acid, wherein the concentration of the stearic acid in the induction liquid is 50-200 micromole/L. According to the induction liquid, liver cell fat hyalinosis is induced through the stearic acid, the modeling success rate is high, the condition requirement is low, the method is simple and convenient, the modeling cycle is show, and the repeatability is high. A targeting tool is offered to research in the occurrence and development of non-alcoholic fatty liver diseases, the liver disease occurrence mechanism is further understood, a new function target point is provided for developing anti-liver injury drugs, and a new thought and a new way for clinically preventing and treating the non-alcoholic fatty liver diseases.