Establishing method of rat atrial fibrillation model for simulating characteristics of human diseases

Abstract

The invention discloses an establishing method of a rat atrial fibrillation model for simulating characteristics of human diseases. The establishing method comprises the steps that 1) an experimentalrat is raised to 35-45 weeks; 2) for the experimental rat, overload of the rat heart pressure is induced by cervical transverse aortic constriction, and a TAC model rat is formed; 3) after the transverse aortic constriction is carried out on the TAC model rat for 1-2 weeks, small animal ultrasonic treatment is conducted to detect a two-dimensional ultrasonic image and color Doppler flow image of blood vessels of a constriction part; 4) after the TAC model rat is constructed for 6-9 weeks, the inner diameter of the left atrium is detected through small animal ultrasonic treatment, the myocardial metabolism is detected by adopting PET, and the occurrence of atrial remodeling is verified; 5) after the transverse aortic constriction is carried out on the TAC model rat for 6-9 weeks, atrial fibrillation is induced through esophageal electrostimulation, a bioelectrical signal recorder is used for recording an electrocardiogram of the experimental rat body surface, and the rat atrial fibrillation model is obtained through verification. The constructed rat atrial fibrillation model can reflect the characteristics of the clinical human diseases, and the establishing method is simpler, moreconvenient, more economical and more efficient.

Description

technical field

[0001] The invention belongs to the medical field, and relates to a method for establishing a mouse atrial fibrillation model, in particular to a method for establishing a mouse atrial fibrillation model for simulating the characteristics of human diseases. Background technique

[0002] The specific mechanism of atrial fibrillation (AF) is not yet clear, in order to study atrial fibrillation, it is necessary to have a suitable animal model of atrial fibrillation. Currently available animal models of atrial fibrillation are divided into two types: one is the atrial fibrillation model induced by atrial overspeed pacing in experimental dogs, but this model needs to be established by experimental dogs, and the experimental dogs themselves have high cost of purchasing experimental animals and the difficulty of making models. It is cumbersome and costly, because the pacemaker implantation in experimental dogs needs to rely on digital subtraction angiography technol...

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