Method for measuring regulation and control of Chemerin on insulin resistance and intervention of CMKLR1 agonist

Abstract

The invention belongs to the technical field of testing or analyzing materials by means of chemical or physical properties of a measuring material, and discloses a method for measuring the regulationand control of Chemerin on insulin resistance and the intervention of a CMKLR1 agonist, which comprises the following steps: preparing a pancreatic diabetes model by adopting an arginine induction method, performing intraperitoneal injection of 350 mg / kg arginine once a day for 6 weeks, and establishing a pancreatic diabetes group by a mouse common diet; during the period, establishing a pancreas-derived diabetes mellitus group by the common diet of the mice;meanwhile, establishing a high-fat diet group by adopting a formula feed open diet; establishinga negative control group, wherein the mice are fed with ordinary diet and water for 6 weeks; adopting a general pathology, an enzyme-linked immunosorbent assay, a Real-Time PCR method and an intraperitoneal injection glucose tolerance test for detection; expressing all the detection data by mean plus or minus standard deviation, and testing the normal distribution is by Shapiro Wilk test; using single-factor variance analysis to comparegroups with each other, and if the groups do not conform to the normal distribution, using Mann-Whitney U test for comparison; significant difference lying in P < 0.05; applying SPSS 22.0 for Windowssoftware to implement the entire verification process.

Description

technical field [0001] The invention belongs to the technical field of testing or analyzing materials by means of measuring their chemical or physical properties, and in particular relates to a method for measuring the regulation of Chemerin on insulin resistance and the intervention of CMKLR1 agonists. Background technique [0002] Currently, the closest prior art: [0003] At present, the animal model of insulin resistance is most commonly induced by a high-fat and high-sugar diet, but the success rate of insulin resistance model induction by this technique is low, the time for successful induction varies, and the controllability is poor. The model is unstable, and the severity of insulin resistance varies, which will lead to large deviations in animal experiments. Using this method to induce animal models to study the regulatory mechanism of proteins such as chemerin is less reliable and requires large samples The amount can effectively reduce the experimental bias. [...

AI Technical Summary

Problems solved by technology

[0005] (1) Low success rate of model induction

[0006] (2) The model is unstable, and the severity of the induced insulin resistance varies

[0007] (3) Using animal models of this type of technology to study the regulatory mechanism of chemerin and other proteins has poor reliability, and requires a large number of samples to reduce experimental bias, low experimental efficiency, and poor repeatability

[0009] Therefore, the method of inducing insulin resistance by allowing animals to eat a high-fat and high-sugar diet freely is poor in controllability. In this field, there is an urgent need to find a relatively standard, good controllability, high induction success rate, and simple and easy-to-operate induction method to replace it.

However, there are relatively large technical difficulties, and the induction method of oral intake is not advisable. It is necessary to design and study other routes and other drugs for induction

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