Method for establishing tissue culture regeneration system of gymnocarpos przewalskii organogenesis pathway
A technology of organogenesis and tissue culture, applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve problems affecting the survival rate of transplanting, and achieve the goal of solving sexual reproduction difficulties, fast differentiation speed, and simple operation Effect
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Embodiment 1
[0035] A method for establishing a tissue culture regeneration system of the phyllocarpus organogenesis pathway, comprising the steps of:
[0036] (1) Disinfection of explants
[0037] Cut the stems of bare fruit trees into short branches, put them in a 2L beaker, wash them with running water for 10 hours, take them out, put them in an ultra-clean workbench, wash them twice with 75% alcohol, wash them twice with sterile water, and disinfect them twice with 3% sodium hypochlorite , 5 minutes each time, wash 6-8 times with sterile water, and dry the water with sterile filter paper;
[0038] (2) Primary callus induction culture
[0039] Inoculate the sterilized stem section into the primary medium with the formula of 1 / 2MS+0.1mg / L IBA+0.05mg / L NAA+30g / L sucrose+7g / L agar, adjust the pH to 5.85-5.95, and the temperature is 25±2℃, light 16h / d, light intensity 2500LX, callus began to form on the 8th day, and the induction rate was over 95%;
[0040] (3) Adventitious bud induction...
Embodiment 2
[0049] A method for establishing a tissue culture regeneration system of the phyllocarpus organogenesis pathway, comprising the steps of:
[0050] (1) Disinfection of explants
[0051] The stems of the bare fruit tree were cut into short branches, placed in a 2L beaker, and rinsed with running water for 10 hours. Take it out, put it into the ultra-clean workbench, wash 2 times with 75% alcohol, wash 2 times with sterile water, disinfect 2 times with 3% sodium hypochlorite, 5 minutes each time, wash 6-8 times with sterile water, and blot dry with sterile filter paper moisture;
[0052] (2) Primary callus culture
[0053] Inoculate the sterilized stem section into the primary medium with the formula of 1 / 2MS+0.1mg / L IBA+0.05mg / L NAA+30g / L sucrose+7g / L agar, adjust the pH to 5.85-5.95, and the temperature is 25±2℃, light 16h / d, light intensity 2500LX, callus began to form on the 8th day, and the induction rate was over 95%;
[0054](3) Adventitious bud induction culture
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