Method for establishing tissue culture regeneration system of gymnocarpos przewalskii organogenesis pathway

A technology of organogenesis and tissue culture, applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve problems affecting the survival rate of transplanting, and achieve the goal of solving sexual reproduction difficulties, fast differentiation speed, and simple operation Effect

Active Publication Date: 2022-01-18
INNER MONGOLIA AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as far as the actual experimental results are concerned, during the rooting process of this recipe, a large number of calluses are formed, which affects the survival rate of subsequent transplants.
No seedling cultivation method has been found to obtain callus from the stem section of the bare fruit tree as explants, and establish a regeneration system that differentiates adventitious buds from the organogenesis pathway

Method used

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  • Method for establishing tissue culture regeneration system of gymnocarpos przewalskii organogenesis pathway
  • Method for establishing tissue culture regeneration system of gymnocarpos przewalskii organogenesis pathway
  • Method for establishing tissue culture regeneration system of gymnocarpos przewalskii organogenesis pathway

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Embodiment 1

[0035] A method for establishing a tissue culture regeneration system of the phyllocarpus organogenesis pathway, comprising the steps of:

[0036] (1) Disinfection of explants

[0037] Cut the stems of bare fruit trees into short branches, put them in a 2L beaker, wash them with running water for 10 hours, take them out, put them in an ultra-clean workbench, wash them twice with 75% alcohol, wash them twice with sterile water, and disinfect them twice with 3% sodium hypochlorite , 5 minutes each time, wash 6-8 times with sterile water, and dry the water with sterile filter paper;

[0038] (2) Primary callus induction culture

[0039] Inoculate the sterilized stem section into the primary medium with the formula of 1 / 2MS+0.1mg / L IBA+0.05mg / L NAA+30g / L sucrose+7g / L agar, adjust the pH to 5.85-5.95, and the temperature is 25±2℃, light 16h / d, light intensity 2500LX, callus began to form on the 8th day, and the induction rate was over 95%;

[0040] (3) Adventitious bud induction...

Embodiment 2

[0049] A method for establishing a tissue culture regeneration system of the phyllocarpus organogenesis pathway, comprising the steps of:

[0050] (1) Disinfection of explants

[0051] The stems of the bare fruit tree were cut into short branches, placed in a 2L beaker, and rinsed with running water for 10 hours. Take it out, put it into the ultra-clean workbench, wash 2 times with 75% alcohol, wash 2 times with sterile water, disinfect 2 times with 3% sodium hypochlorite, 5 minutes each time, wash 6-8 times with sterile water, and blot dry with sterile filter paper moisture;

[0052] (2) Primary callus culture

[0053] Inoculate the sterilized stem section into the primary medium with the formula of 1 / 2MS+0.1mg / L IBA+0.05mg / L NAA+30g / L sucrose+7g / L agar, adjust the pH to 5.85-5.95, and the temperature is 25±2℃, light 16h / d, light intensity 2500LX, callus began to form on the 8th day, and the induction rate was over 95%;

[0054](3) Adventitious bud induction culture

[0...

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Abstract

The invention discloses a method for establishing a tissue culture regeneration system of a gymnocarpos przewalskii organogenesis pathway, and belongs to the technical field of forestry vegetative propagation. The method comprises the following steps: (1) explant disinfection; (2) primary callus induction culture; (3) adventitious bud induction culture; (4) subculture of adventitious buds; (5) rooting culture; and (6) seedling regeneration. According to the method for establishing the tissue culture regeneration system of the gymnocarpos przewalskii organogenesis pathway, materials are convenient to obtain, operation is easy and convenient, the growth cycle of seedlings is short, the method is not affected by seasons, tissue culture seedlings of gymnocarpos przewalskii can be rapidly cultivated in a short time, the regeneration system is established, the problem that sexual propagation of the gymnocarpos przewalskii is difficult is solved, and meanwhile, a new way is provided for good germplasm resource cultivation, gene transformation, genetic resource preservation and industrialized seedling rearing of the gymnocarpos przewalskii.

Description

technical field [0001] The invention relates to a method for establishing a tissue culture regeneration system of the organogenesis pathway of bare fruit trees, belonging to the technical field of forestry asexual reproduction. Background technique [0002] Gymnocarpos przewalskii Maxim (scientific name: Gymnocarpos przewalskii Maxim) belongs to the genus Gymnocarpos przewalskii Maxim, a perennial dwarf shrub with many branches, linear leaves, hermaphroditic flowers, and small pollen. It is a relic plant of the ancient Mediterranean xerophyte flora. It is distributed in Gansu, Qinghai, western Inner Mongolia and other places. At the same time, because most of its distribution areas are fragile ecosystems, and it is one of the sources of animal food in desert areas, the number of bare fruit trees is decreasing day by day. In 1987 and 1997, it was recognized as a national second-level and first-level protected plant. [0003] The natural seed setting rate of bare fruit trees ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002Y02P60/40
Inventor 杨海峰王佳琪萨如拉王树森甘晓雪郝璞张新乾樊俐娇刘璐查苏谢丕青王佳濛
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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