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Establishment method of endoplasmic reticulum stress induced mouse acute liver injury model

A technique for endoplasmic reticulum stress and acute liver injury, which can be applied in pharmaceutical formulations, organic active ingredients, medical preparations containing active ingredients, etc., and can solve problems such as experimental animal models without acute liver injury.

Inactive Publication Date: 2014-04-23
BENGBU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no experimental animal model of acute liver injury caused by the induction of endoplasmic reticulum stress in the above liver injury models

Method used

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  • Establishment method of endoplasmic reticulum stress induced mouse acute liver injury model
  • Establishment method of endoplasmic reticulum stress induced mouse acute liver injury model
  • Establishment method of endoplasmic reticulum stress induced mouse acute liver injury model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] To prepare an endoplasmic reticulum stress inducer, take 0.5 mg tunicamycin powder with a purity greater than 99% and dissolve it in 100 μl dimethyl sulfoxide (DMSO) with a purity greater than 99.8% to prepare a 0.005 mg / μl stock solution; Take 100μl storage solution and add 19.9ml double distilled water (ddH 2 O) to 20ml, that is, after diluting the stock solution 200 times, complete the preparation of the endoplasmic reticulum stress inducer, and store it in a -20°C environment protected from light for future use.

[0035] Follow the steps below to establish a mouse model of acute liver injury:

[0036] a) The mice in group C were randomly divided into four comparison groups, ten mice in each comparison group, and the above-mentioned endoplasmic reticulum stress inducer was administered to the mice in group C, and the amount of endoplasmic reticulum stress inducer was The dosage is 0.2ml / 10g;

[0037] b) According to different chronological order, the mice in the fo...

Embodiment 2

[0043] To prepare an endoplasmic reticulum stress inducer, take 1 mg tunicamycin powder with a purity greater than 99% and dissolve it in 100 μl dimethyl sulfoxide (DMSO) with a purity greater than 99.8% to prepare a 0.01 mg / μl stock solution; 100μl storage solution plus 19.9ml double distilled water (ddH 2 O) to 20ml, that is, after diluting the stock solution 200 times, complete the preparation of the endoplasmic reticulum stress inducer, and store it in a -20°C environment protected from light for future use.

[0044] Follow the steps below to establish a mouse model of acute liver injury:

[0045] a) The mice in group D were randomly divided into four comparison groups, ten mice in each comparison group, and the above-mentioned endoplasmic reticulum stress inducer was administered to the mice in group D, and the amount of endoplasmic reticulum stress inducer was The dosage is 0.2ml / 10g;

[0046] b) According to different chronological order, the mice in the four comparis...

Embodiment 3

[0049] To prepare an endoplasmic reticulum stress inducer, take 2 mg tunicamycin powder with a purity greater than 99% and dissolve it in 100 μl dimethyl sulfoxide (DMSO) with a purity greater than 99.8% to prepare a 0.02 mg / μl stock solution; 100μl storage solution plus 19.9ml double distilled water (ddH 2 O) to 20ml, that is, after diluting the stock solution 200 times, complete the preparation of the endoplasmic reticulum stress inducer, and store it in a -20°C environment protected from light for future use.

[0050]Follow the steps below to establish a mouse model of acute liver injury:

[0051] a) The mice in group E were randomly divided into four comparison groups, ten mice in each comparison group, and the above-mentioned endoplasmic reticulum stress inducer was administered to the mice in group E, and the amount of endoplasmic reticulum stress inducer was The dosage is 0.2ml / 10g;

[0052] b) According to different chronological order, the mice in the four compariso...

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Abstract

The invention provides an establishment method of an endoplasmic reticulum stress induced mouse acute liver injury model. The establishment method orderly comprises the following steps: carrying out intragastric administration treatment on mice by use of an endoplasmic reticulum stress inducer with a dosage of 0.2ml / 10g for each mouse, and completing the establishment of the mouse acute liver injury model in 8-48 hours after administration, wherein the endoplasmic reticulum stress inducer is prepared by a method comprising the steps of taking (0.5-4)mg of tunicamycin for dissolving 100 microliters of dimethyl sulfoxide to obtain a preservation solution, taking 100 microliters of preservation solution and adding 19.9ml of double distilled water to 20ml, and thus completing the preparation of the endoplasmic reticulum stress inducer. The mouse model of the induced acute liver injury is established by inducing the endoplasmic reticulum stress effect; through the study on the mechanism of action of the endoplasmic reticulum stress, the self regulation ability of cells in the stress state can be further known, and the occurrence mechanism of the liver diseases can be further known; therefore, new intervention and treatment measures can be adopted for the liver diseases to achieve the purposes of preventing and treating diseases.

Description

technical field [0001] The invention relates to a method for establishing a mouse acute liver injury model induced by endoplasmic reticulum stress. Background technique [0002] The acute liver injury model in experimental animals is an indispensable tool for studying the pathogenesis of various liver diseases and screening for therapeutic drugs. It has been found that the occurrence and development of many liver diseases are related to endoplasmic reticulum stress and its mediated cell apoptosis, including viral hepatitis, alcoholic liver disease, non-alcoholic steatohepatitis, drug-induced liver disease, acute liver failure , liver cancer, etc. [0003] There are many ways to create experimental liver injury animal models. Although the research on liver injury models at home and abroad has made significant progress in recent years, due to the complexity of liver function and the diversity of liver injury factors, any experimental model cannot be comprehensive and accurate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7072
Inventor 刘浩储菲李晓明董清清蒋玉敏
Owner BENGBU MEDICAL COLLEGE
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