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Improved SD (Sprague Dawley) rat CD (Crohn's Disease) modeling method

A technique of rat Crohn's disease and modeling

Inactive Publication Date: 2017-06-13
GUANGZHOU ZHONGDA NANSHA TECH INNOVATION IND PARK +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the disadvantage is that there is no acute phase performance, and the mortality rate of animals is relatively high

Method used

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  • Improved SD (Sprague Dawley) rat CD (Crohn's Disease) modeling method
  • Improved SD (Sprague Dawley) rat CD (Crohn's Disease) modeling method
  • Improved SD (Sprague Dawley) rat CD (Crohn's Disease) modeling method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Improved SD rat Crohn's disease modeling method

[0027] 1. Preparation of modeling drugs

[0028] Take a certain volume of 5% TNBS aqueous solution and mix it with an equal volume of absolute ethanol, and store it at 4°C until use.

[0029] 2. Modeling method

[0030] (1) SD rats weighed 160g-220g and were fasted for 24 hours before modeling.

[0031] (2) Weigh and record the body weight before modeling, inject 0.3ml / l00g 10% chloral hydrate intraperitoneally after anesthesia, and inject PBS to wash the intestinal tract.

[0032] (3) Insert the gavage needle from the anus 8cm away from the anus, and inject 50mg / kg of modeling drugs (5% TNBS and absolute ethanol are mixed at a volume ratio of 1:1, 0.2ml / 100g body weight). After introducing the modeling liquid, adopt a posture with the head lowered and the tail higher to prevent the overflow of the perfusion liquid, and lift the rat upside down for 1 to 2 minutes. SD rats in the control group were given an ...

Embodiment 2

[0053] Example 2 Improved SD rat Crohn's disease modeling method

[0054] 1. Preparation of modeling drugs

[0055] Take a certain volume of 1% TNBS aqueous solution and mix it with an equal volume of absolute ethanol, and store it at 4°C until use.

[0056] 2. Modeling method

[0057] (1) SD rats weighed 160g-220g and were fasted for 24 hours before modeling.

[0058] (2) Weigh and record the body weight before modeling, inject 0.3ml / l00g 10% chloral hydrate intraperitoneally after anesthesia, and inject PBS to wash the intestinal tract.

[0059] (3) Insert the gavage needle from the anus 8cm away from the anus, and inject 50mg / kg of modeling drug (1% TNBS and absolute ethanol are mixed at a volume ratio of 1:1, 0.2ml / 100g body weight). After introducing the modeling drug solution, adopt a position with the head lowered and the tail higher to prevent the overflow of the perfusion fluid, and lift the rat upside down for 1-2 minutes; the SD rats in the control group were giv...

Embodiment 3

[0064] Example 3 Improved SD Rat Crohn's Disease Modeling Method

[0065] 1. Preparation of modeling drugs

[0066] Take a certain volume of 10% TNBS aqueous solution and mix it with an equal volume of absolute ethanol, and store it at 4°C until use.

[0067] 2. Modeling method

[0068] (1) SD rats weighed 160g-220g and were fasted for 24 hours before modeling.

[0069] (2) Weigh and record the body weight before modeling, inject 0.3ml / l00g 10% chloral hydrate intraperitoneally after anesthesia, and inject PBS to wash the intestinal tract.

[0070] (3) Insert the gavage needle from the anus 8cm away from the anus, and inject 50mg / kg of modeling drug (5% TNBS and absolute ethanol are mixed at a volume ratio of 1:1, 0.1ml / 100g body weight). After introducing the modeling drug solution, adopt a position with the head lowered and the tail higher to prevent the overflow of the perfusion fluid, and lift the rat upside down for 1-2 minutes; the SD rats in the control group were gi...

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Abstract

The invention belongs to the technical field of animal disease models, and specifically discloses an improved SD (Sprague Dawley) rat CD (Crohn's Disease) modeling method. The improved SD rat CD modeling method comprises the following steps: after fasting and narcotizing an SD rat, carrying out clysis by using a modeling drug (which is prepared by mixing 5 percent of a TNBS (Trinitro-Benzene-Sulfonic Acid) water solution with absolute ethyl alcohol according to a volume ratio of 1 to 1 and is 0.2 ml / 100 g in body mass) according to the dosage of 50 mg / kg, adopting a body position of enabling the head of the SD rat to face downwards and the tail to face upwards after leading in the modeling drug so as to prevent clysis liquid from flowing outwards, and reversely carrying the SD rat for 1 to 2 minutes. The improved SD rat CD modeling method disclosed by the invention is simple and convenient in operation and good in repeatability, and a model accords with the features such as diarrhea, bloody stools, intestinal obstruction, weight lose, colonic ulcers and adhesion and abscess between intestinal canals and between an intestinal canal and visceral organs or tissues of clinic human CD; the modeling success rate is high, and the animal death rate is low.

Description

technical field [0001] The invention belongs to the technical field of animal disease models, and in particular relates to an improved SD rat Crohn's disease modeling method, which can be better used for preliminary screening of drugs for treating Crohn's disease. Background technique [0002] Crohn's disease (CD) is a chronic granulomatous inflammatory disease of the gastrointestinal tract mainly caused by immune response disorders. At present, in our country, its incidence rate is increasing year by year. Chronic inflammation and injury of intestinal tract can lead to serious complications such as intestinal fibrosis and even intestinal obstruction. At present, there is still a lack of specific and effective drugs for the treatment of CD. Therefore, exploring the pathogenesis of CD to seek new therapeutic targets has become a hot topic. [0003] Combining trinitrobenzenesulfonic acid (TNBS) with ethanol to create animal models of Crohn’s Disease (CD) is a commonly used mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/185A61K31/045A61P1/00A61P29/00A61P37/02
CPCA61K31/045A61K31/185
Inventor 姜福林钟国平黄民
Owner GUANGZHOU ZHONGDA NANSHA TECH INNOVATION IND PARK
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