Method for making primate dry age-related macular degeneration disease model

Abstract

The invention discloses a method for making a dry age-related macular degeneration disease model on a primate, namely applying sodium iodate onto the primate with a carotid artery injection method. The method is simple and easy, high in success rate and good in repeatability. The model made with the method is stable. The amount of sodium iodate needed is small, injury of large doses of sodium iodate to the whole body of the animal is avoided, and the animal can live for a long time so that follow-up tests can be made possible. A model of any one side or a model of double sides can be made as needed. When the one-side model is made, a lateral eye can not be hurt at all, and normal living vision of the animal is guaranteed. Comparison of two lateral eyes of one animal can be conducted to provide credibility for the test, and the number of animals needed for the test is reduced. The method provides a sound tool for the dry age-related macular degeneration research and has great significance for the pathological physiology research and treatment research of the disease.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for making a primate dry age-related macular degeneration model. Background technique [0002] Dry age-related macular degeneration (age-related macular degeneration, AMD) is one of the common blinding eye diseases in middle-aged and elderly people. Studies have shown that the normal function of the retinal pigment epithelium (RPE) is of vital importance to the number and function of photoreceptor cells. The loss of cells is secondary. As the age increases, the incidence of AMD gradually increases, so its harm is increasing with the aging of our population. There is still no clear and effective treatment for dry age-related macular degeneration, and its pathogenesis and treatment methods have become research hotspots. However, the lack of an ideal animal disease model has greatly hindered its research progress. [0003] Sodium iodate is an anti-metabolite, which can ...

AI Technical Summary

Problems solved by technology

Due to the method of intravenous injection of sodium iodate for modeling, the drug first returns to the heart through the vein, and then is pumped out through the heart through the aorta, carotid artery, and ophthalmic artery into the retina-choroidal circulation, and the drug passes through the systemic circulation to make the drug concentration in the eyes It has been greatly reduced, and the effect of the drug on the pigment epithelium is concentration-dependent. In order to achieve the purpose of destroying the pigment epithelium, the dosage should be increased, but this will correspondingly increase the systemic toxicity of the drug, leading to hematuria, renal failure and even Death without corresponding retinal pigment epithelial lesions evident

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