71results about How to "Long-term survival" patented technology

The combination of probiotics and food is helpful for inhibiting the growth of pathogens and absorbing special nutritions. However, the probiotics need to survive at high temperature in the process of food processing, and after the probiotics enter the body, the probiotics must go through the test of stomach acid and bile salt to reach the intestines. Accordingly, the invention provides a bacillus subtilis strain BS139, which is characterized by the survivability of resisting environmental stresses, including the characteristics such as acid resistant, bile salt resistant, high temperature resistance and the like. The probiotic strain is preserved in Agricultural Research Service, NRRL and has a preservation number of NRRL No.B-50347, with further applications in the preparation of food commodities, pharmaceutical compositions, and daily necessities.

The invention provides to a microorganism-containing fertilizer produced by a mycoderm method and a production method of the fertilizer. In the prior art, microorganism bactericides, organic matters, chemical fertilizer and the like are mixed according to a preset formula, and the mixture is subjected to granulation, high-temperature drying, screen separation, cooling and the like so as to produce a finished product. In the production method provided by the invention, the fertilizer without bactericides is granulated, dried, screened and cooled; active bacterium powder is coated on the outer surface of the fertilizer particles at the tail end of the production line by using a film technology; and the microbial content of the whole fertilizer when in detection reaches the technical standard of national regulation so that a film fertilizer is formed. The method provided by the invention is used for producing the fertilizer with active bacterium.

Engineered human tissue constructs are provided that are suitable for use in making humanized animals for use in pharmaceutical development. Humanized animals having the constructs implanted in vivo are provided. Methods of making and using the tissue-engineered constructs and humanized animals are also provided.

The present invention provides improved treatment methods by the administration of both an inhibitor of indoleamine-2,3-dioxygenase in addition to the administration of an additional therapeutic agent.

A process for preparing a non-woven disinfecting strip of Metarrhizium anisopliae includes sampling the bacterial strain, slant culture, liquid culture, production with fermentor, and inoculating the Metarrhizium anisopliae to non-woven strips. Its advantages are high and durable effect on pests (such as longicorn), low cost and no environmental pollution.

The invention relates to freezing and thawing of MDCK cell, and relates to freezing and thawing of the MDCK cell. The freezing method comprises the following steps: by restricting the addition rate of the frozen stock solution in mixing of a frozen stock solution and the cell, cell damage is prevented, by adjusting the cooling rate, generation of ice crystal in the cell can be prevented, and cell death is lead. In cell thawing, thawing can be rapidly carried out, and integrity and survival rate of the cell can be guaranteed. The method can better keep the MDCK cell vitality. The cell vitality after thawing can reach more than 95%, the cell quality is guaranteed, and long-term viability of the cell can be guaranteed.

The invention discloses a method for making a dry age-related macular degeneration disease model on a primate, namely applying sodium iodate onto the primate with a carotid artery injection method. The method is simple and easy, high in success rate and good in repeatability. The model made with the method is stable. The amount of sodium iodate needed is small, injury of large doses of sodium iodate to the whole body of the animal is avoided, and the animal can live for a long time so that follow-up tests can be made possible. A model of any one side or a model of double sides can be made as needed. When the one-side model is made, a lateral eye can not be hurt at all, and normal living vision of the animal is guaranteed. Comparison of two lateral eyes of one animal can be conducted to provide credibility for the test, and the number of animals needed for the test is reduced. The method provides a sound tool for the dry age-related macular degeneration research and has great significance for the pathological physiology research and treatment research of the disease.

The invention discloses a method for repairing DEHP contaminated soil and lowering the DEHP content in growing vegetables with Microbacterium sp.J-1. In particular, flowering cabbage is planted in the DEHP contaminated soil, and a Microbacterium sp.J-1 strain is inoculated around a root system of the flowering cabbage. If the Microbacterium sp.J-1 strain is only inoculated into the DEHP contaminated soil, a persistent repairing effect cannot be achieved for the reason that the Microbacterium sp.J-1 strain can die with the extension of inoculation time. However, if the Microbacterium sp.J-1 strain is inoculated into the DEHP contaminated soil, and meanwhile the flowering cabbage is planted, the root system environment of the flowering cabbage provides available organic matter for the Microbacterium sp.J-1 strain, the inoculated strain can survive in the soil for a long time, and the persistent repairing effect on the contaminated soil can be achieved. Meanwhile, the flowering cabbage with the quality safety up to the standard can be planted, and the mode of 'repairing while production' is realized.

Methods of identifying compounds that modulate the conversion of AMP to adenosine by 5′-nucleotidase, ecto, and that possess particular pharmacokinetic characteristics are described herein. Methods of such compounds, and compositions comprising same, for the treatment and/or prevention of a diverse array of diseases, disorders and conditions, including cancer- and immune-related disorders, are also provided.

Compounds that modulate the conversion of AMP to adenosine by 5′-nucleotidase, ecto, and compositions containing the compounds and methods for synthesizing the compounds, are described herein. The use of such compounds and compositions for the treatment and/or prevention of a diverse array of diseases, disorders and conditions, including cancer- and immune-related disorders, that are mediated by 5′-nucleotidase, ecto is also provided.

Crystalline forms of the compound of Formula (I), which modulates the conversion of AMP to adenosine by 5′-nucleotidase, ecto, and compositions containing the compound and methods for preparing the crystalline forms, are described herein. The use of such crystalline form of Formula (I) and compositions for the treatment and/or prevention of a diverse array of diseases, disorders and conditions, including cancer and immune-related disorders, that are mediated by 5′-nucleotidase, ecto is also provided.

The invention provides a protein composite scaffold capable of self-producing oxygen. The protein composite scaffold capable of self-producing oxygen comprises bioprotein, gelatin and an oxygen self-producing material. A mass ratio of the gelatin to the bioprotein is (0.5-5):10 and a mass ratio of the oxygen self-producing material to the bioprotein is (1-50):10. The protein composite scaffold capable of self-producing oxygen is compounded from the bioprotein, the gelatin and the oxygen self-producing material, and has physical strength superior to a membrane structure material constructed by using a pure protein combined solution. Meanwhile, the gelatin has a certain coating effect on the oxygen self-producing material, so that the provided protein composite scaffold can release oxygen at a constant speed and a high level; seed cells compounded on the protein composite scaffold can survive for a long time and microvessel ingrowth after the material is implanted is promoted. The protein composite scaffold is suitable for requirements of actual surgery operations, and finally increases success rate of urethral repair and reconstruction.

The invention provides a landscape water-culture fish-vegetable coexistence method and relates to the technical field of aquaculture and soilless cultivation. Fish-vegetable coexistence nutritional liquid comprises calcium nitrate, potassium dihydrogen phosphate, potassium sulfate, magnesium sulfate, potassium nitrate, chelated iron, boric acid, manganese sulfate, zinc sulfate, copper sulfate, ammonium molybdate and water, so that vegetables can be ensured to get nutrient; fish-type edible vegetable root systems are added, and additional feeding of feed is not needed, so that water cleanlinessis ensured to enable fishes to survive for a long time. A fish-vegetable coexistence state using the nutritional liquid prepared from chemical preparations, with fishes and vegetable root systems ina same space and having good landscape effect is formed finally. By applying the fish-vegetable coexistence nutritional liquid and combining with nutritional liquid parameter control and a proper fish-vegetable coexistence planting and breeding system, the fishes directly growing in the nutritional liquid can survive normally for a long time, and vegetables can normally grow.

The invention relates to an extraction method of an active factor for improving a survival rate of transplanted autologous fat such as autologous fat living cells, which comprises the following steps:1) putting extracted adipose tissue mixed liquid into a test tube, centrifuging, sucking supernatant plasma on the uppermost layer, adding a hydroxyethyl starch solution, and sufficiently and uniformly mixing, 2) adding into a nucleic acid type fermentation liquid, adding normal saline, stirring, standing, extracting fermentation liquid supernatant by using a sterile injector, adding a cryopreservation liquid into the fermentation liquid supernatant, and freezing by using semiconductor refrigeration equipment, (3) carrying out centrifugal operation by using a freezing centrifugal machine, after the fermentation liquor is liquefied again, adding the liquid after standing into a centrifugal tube, and carrying out secondary centrifugal treatment on the centrifugal tube in the centrifugal machine, 4) after centrifugal filtration, putting into a refrigerated centrifuge for centrifugal operation, and adding a new plasma sample to enable precipitated platelets to suspend again to obtain theactive factor. By means of the method, original adipose cells can be promoted to continue to grow and finally become adipose tissue stably, and the survival rate is high.