Anti-saline-alkali aspergillus ochratensis strain W1 and micro-organism agent thereof, and applications of anti-saline-alkali aspergillus ochratensis strain W1

A saline-alkali-resistant technology of ochratoxin, applied in the saline-alkali-resistant ochratobacter strain W1 and its bacterial agent and application field, which can solve the problem that the strain is difficult to play a role

Active Publication Date: 2019-11-26
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the current microbial agents can play a good role in normal soil, but in saline-alkali soil with high concentration of salt and high pH, ​​it is difficult for these strains to play their due role

Method used

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  • Anti-saline-alkali aspergillus ochratensis strain W1 and micro-organism agent thereof, and applications of anti-saline-alkali aspergillus ochratensis strain W1
  • Anti-saline-alkali aspergillus ochratensis strain W1 and micro-organism agent thereof, and applications of anti-saline-alkali aspergillus ochratensis strain W1
  • Anti-saline-alkali aspergillus ochratensis strain W1 and micro-organism agent thereof, and applications of anti-saline-alkali aspergillus ochratensis strain W1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, the isolation of highly saline-alkali-resistant ochracus strain

[0029] The soil straw samples for the isolated strains were from Zhenlai County, Baicheng City, Jilin Province. Select the plots that have been desolate for many years and are highly salinized in this area, and take about 5 cm of soil straw from the soil surface, put them into sterilized glass bottles, and bring them back to the laboratory for storage at 4 °C for later use. Using the flat plate dilution coating method, the diluted soil supernatant was spread on the PDA medium added with NaCl to 250.0g / L, the pH was 10, cultured for 4 days, and a plant with high salt-alkali resistance was isolated after 3 rounds of screening The fungal strains of the highly saline-alkali resistant type are numbered CGMCC NO.17971.

Embodiment 2

[0030] Embodiment 2, identification of highly saline-alkali-resistant bacterial strain

[0031] Morphological identification: Inoculate the screened highly saline-alkali-resistant strain W1 on PDA medium containing 5% NaCL, culture at 30°C for 5 days, and observe the colony morphology. Pick up the sterilized coverslip with tweezers and insert it obliquely into the middle of 5% NaCL PDA medium. Inoculate a bacterial slice with a diameter of 7mm directly in front of the cover glass, with the mycelium facing down, then place the plate in a constant temperature incubator at 30°C, and observe the growth of the mycelium regularly. After the mycelium climbs up the cover glass, put The culture medium connected with the cover glass was cut off, the cover glass was taken out, and the hyphae and spore morphology were observed under a microscope. After culturing on PDA medium containing 5% NaCL at 30°C for 5 days, the diameter of the round colony is about 67-73.8 mm, it is white, the sur...

Embodiment 3

[0033] Example 3, identification of high salt resistance and optimum sodium salt concentration of bacterial strain Ochraus persicae W1

[0034]1, prepare the PDA medium of gradient NaCl content, pH is 7.0, and its salt concentration (mass fraction) increases to 31% (saturation concentration) by 0%, every level increases by 2%. An equal amount of strains were inoculated in the center of the culture medium and cultured at 30°C for 1 week. It was found that the strains could grow in 0%-31% NaCl PDA medium at a temperature of 30°C, but the growth of the strains was significantly inhibited as the salt concentration increased. The growth is relatively slow at the concentration of 1%-5%; the growth of the strain is accelerated at the concentration of 5%-13%, the growth of the strain is sharply slowed at the concentration of 15%-25%, and the bacteria basically do not grow at the concentration of 27%-31%. Therefore, the strain has salt-resistant properties and belongs to fungi with hi...

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Abstract

The present invention relates to anti-saline-alkali aspergillus ochratensis strain W1 and a micro-organism agent thereof, and applications of the anti-saline-alkali aspergillus ochratensis strain W1.The strain is aspergillus ochratensis preserved in China General Microbiological Culture Collection Center and has a preservation name of aspergillus ochratensis W1 and a preservation number of CGMCCNO.17971. The anti-saline-alkali aspergillus ochratensis strain W1 can stably survive in the soil with high sodium salt concentration and high pH, effectively degrades straws and is suitable for rebuilding saline-alkali land.

Description

technical field [0001] The invention belongs to the field of microbial engineering and technology, and in particular relates to a saline-alkali-resistant ochracus strain W1 and its bacterial agent and application. Background technique [0002] In our large agricultural country, the annual output of straw is more than 700 million tons. From 1991 to the present, the state has issued a series of relevant documents on the utilization of crop straws, with the purpose of promoting the resource utilization of crop straws and strictly prohibiting the burning of crop straws. However, the resource utilization rate of straw is low, and people's comprehensive utilization of straw is mainly concentrated in simple and extensive treatment methods, such as traditional straw fertilizer, feed, fuel, or even directly discarded and incinerated, and used as industrial raw materials, energy Insufficient research on fine treatment methods such as chemical treatment has led to the increasingly com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B09C1/10C05F11/08C12R1/66
CPCC12N1/14B09C1/105C05F11/08C12R2001/66C12N1/145
Inventor 魏毅唐紫琪张舒涵巩志远李柱刚张世宏
Owner JILIN UNIV
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