Extraction method of active factor for improving survival rate of transplanted autologous fat such as autologous fat living cells

A technology of active factors and living cells, applied in the field of biological cells, can solve the problems of necrosis and high survival rate, and achieve the effects of accelerating vascularization and resurvival, ensuring vitality, and promoting angiogenesis factors

Pending Publication Date: 2020-10-30
乔爱军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the above-mentioned deficiencies and provide a method for extracting active factors that improve the survival rate of transplanted autologous fat such as autologous fat living cells, which can promote the continued growth of original fat cells, and finally stabilize them into adipose tissue, and The survival rate is high, which solves the problems of traditional fat transplantation absorption and active fat cell transplantation absorption or necrosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for extracting active factors for improving the survival rate of transplanted autologous fat such as autologous fat living cells, comprising the following steps:

[0019] 1) Under sterile conditions, put the extracted adipose tissue mixture into a test tube, and centrifuge it in a centrifuge for 15 minutes at a speed of 1200rpm, so that the whole blood sample is initially separated. After centrifugation, the blood is added with anticoagulant, statically Leave it for 30-60 minutes, discard the grease layer, absorb the uppermost layer of supernatant plasma, add hydroxyethyl starch solution and mix well; among them, the ratio of anticoagulant to whole blood sample is 1:10, and the anticoagulant is B Made by mixing diaminetetraacetic acid, sodium oxalate, heparin and sodium citrate;

[0020] 2) Add the sample obtained in step 1) into the nucleic acid-based fermentation broth to obtain the sample fermentation broth, add physiological saline to stir, and let it stand...

Embodiment 2

[0024] A method for extracting active factors for improving the survival rate of transplanted autologous fat such as autologous fat living cells, comprising the following steps:

[0025] 1) Under sterile conditions, put the extracted adipose tissue mixture into a test tube, and centrifuge it in a centrifuge for 15 minutes at a speed of 1200rpm, so that the whole blood sample is initially separated. After centrifugation, the blood is added with anticoagulant, statically Leave it for 30-60 minutes, discard the grease layer, absorb the uppermost layer of supernatant plasma, add hydroxyethyl starch solution and mix well; among them, the ratio of anticoagulant to whole blood sample is 1:12, and the anticoagulant is B Made by mixing diaminetetraacetic acid, sodium oxalate, heparin and sodium citrate;

[0026] 2) Add the sample obtained in step 1) into the nucleic acid-based fermentation broth to obtain the sample fermentation broth, add physiological saline to stir, and let it stand...

Embodiment 3

[0030] A method for extracting active factors for improving the survival rate of transplanted autologous fat such as autologous fat living cells, comprising the following steps:

[0031] 1) Under sterile conditions, put the extracted adipose tissue mixture into a test tube, and centrifuge it in a centrifuge for 15 minutes at a speed of 1200rpm, so that the whole blood sample is initially separated. After centrifugation, the blood is added with anticoagulant, statically Leave it for 30-60 minutes, discard the grease layer, absorb the uppermost layer of supernatant plasma, add hydroxyethyl starch solution and mix well; among them, the ratio of anticoagulant to whole blood sample is 1:10, and the anticoagulant is B Made by mixing diaminetetraacetic acid, sodium oxalate, heparin and sodium citrate;

[0032] 2) Add the sample obtained in step 1) into the nucleic acid-based fermentation broth to obtain the sample fermentation broth, add physiological saline to stir, and let it stand...

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Abstract

The invention relates to an extraction method of an active factor for improving a survival rate of transplanted autologous fat such as autologous fat living cells, which comprises the following steps:1) putting extracted adipose tissue mixed liquid into a test tube, centrifuging, sucking supernatant plasma on the uppermost layer, adding a hydroxyethyl starch solution, and sufficiently and uniformly mixing, 2) adding into a nucleic acid type fermentation liquid, adding normal saline, stirring, standing, extracting fermentation liquid supernatant by using a sterile injector, adding a cryopreservation liquid into the fermentation liquid supernatant, and freezing by using semiconductor refrigeration equipment, (3) carrying out centrifugal operation by using a freezing centrifugal machine, after the fermentation liquor is liquefied again, adding the liquid after standing into a centrifugal tube, and carrying out secondary centrifugal treatment on the centrifugal tube in the centrifugal machine, 4) after centrifugal filtration, putting into a refrigerated centrifuge for centrifugal operation, and adding a new plasma sample to enable precipitated platelets to suspend again to obtain theactive factor. By means of the method, original adipose cells can be promoted to continue to grow and finally become adipose tissue stably, and the survival rate is high.

Description

[technical field] [0001] The invention relates to the technical field of biological cells, in particular to a method for extracting active factors for improving the survival rate of transplanted autologous fat such as autologous fat living cells. [Background technique] [0002] Fat is mainly distributed in human subcutaneous tissue, omentum, mesentery and perirenal and other parts. Adipose tissue is composed of preadipocytes and mature adipocytes. The patina in mature adipocytes contains 90% lipid droplets, which can be quickly metabolized after transplantation. Mature adipocytes are terminally differentiated cells that have lost the ability to continue proliferating. Mature adipocytes can only increase the volume of the transplanted site and mature adipocytes are vulnerable to external mechanical damage, so that a considerable number of mature adipocytes are damaged and destroyed. Commonly used fat grafting is processed fat granules to remove impurities such as oil, blood...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3604A61L27/3687A61L27/3691A61L27/50A61L2400/06A61L2430/34A61L2430/40
Inventor 乔爱军
Owner 乔爱军
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