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Method for repairing DEHP contaminated soil and lowering DEHP content in growing vegetables with Microbacterium sp.J-1

A technology for polluting soil and vegetables, applied in the field of biological treatment of environmental pollutants, can solve problems such as rarely polluting farmland soil

Active Publication Date: 2017-06-06
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the reported PAEs degrading bacteria are limited to shake flask degradation research in the laboratory, and are rarely applied to the safe production of polluted farmland soil

Method used

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  • Method for repairing DEHP contaminated soil and lowering DEHP content in growing vegetables with Microbacterium sp.J-1
  • Method for repairing DEHP contaminated soil and lowering DEHP content in growing vegetables with Microbacterium sp.J-1
  • Method for repairing DEHP contaminated soil and lowering DEHP content in growing vegetables with Microbacterium sp.J-1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The optimization of fermentation conditions of Microbacterium sp.J-1 degrading bacteria and the preparation of freeze-dried bacterial agent, the specific method is as follows:

[0032] 1. Optimization of fermentation conditions: Culture the strains stored at 4°C on a slant and activate at 30°C for 24 hours. Use 10mL of sterile water to make the activated bacterial strain into a bacterial suspension, and the OD 600 The inoculum size of =1.0 was placed in the seed liquid medium, and cultured with constant temperature shaking at 30°C and 180rpm for 24h; 600 =1.0 inoculum size, put the seed solution into the shake flask liquid fermentation medium (containing 200mg / LDEHP), cultivate it at 30°C and 180rpm, and take a sample every 24h, the initial pH of the strain activation condition (6~10) , temperature (20-40°C), and inoculum size (0.2-1.0) were optimized by response surface surface (RSM). The Box-Behnken experimental design was adopted, and three levels of high, medium a...

Embodiment 2

[0056] Experiment on the effect of Microbacterium sp.J-1 bacterial agent on the degradation effect of DEHP in polluted soil and the content of DEHP in planted Chinese cabbage

[0057] 1. Preparation of test soil and selection of vegetables for planting: the soil is paddy soil from the farm of South China Agricultural University, passed through a 60-mesh sieve after air-drying, and the pH is 6.7. Add DEHP to it to reach 50mg / kg, and add double distilled water to adjust to the field water holding capacity (about 30%), avoid light aging for 15 days. Our previous research showed that Brassica parachinensis L. variety Huaguanyouqing has the characteristics of high accumulation of DEHP, which poses a serious threat to food safety and human health.

[0058] 2. Experimental treatment: four treatments were set up: blank contaminated soil (CK), inoculated contaminated soil (inoculation strain), planted Chinese cabbage contaminated soil (planted Chinese cabbage), and simultaneously inocu...

Embodiment 3

[0066] A method for repairing DEHP-contaminated soil while reducing DEHP content in vegetables grown in contaminated soil, comprising the steps of:

[0067] (1) Activate the Microbacterium sp.J-1 strain and carry out fermentation culture. The fermentation temperature is 30°C, the pH is 8.0, and the inoculum size is OD 600 =0.7;

[0068](2) The fermented liquid obtained in step (1) is centrifuged at a rotating speed of 5000rpm for 10min, and the thallus is collected. According to the addition of 6mg glucose / g thallus, glucose is added to the thalline and then put into a low-temperature freezer for pre-freezing. After freezing completely and forming small ice crystals, put the pre-frozen bacterial liquid into a vacuum freeze dryer with a cold trap temperature of -30°C for 24 hours, and collect the freeze-dried bacterial cells.

[0069] (3) After planting Chinese cabbage in DEHP-contaminated soil, inoculate the freeze-dried bacterial agent around the root system of Chinese cabba...

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Abstract

The invention discloses a method for repairing DEHP contaminated soil and lowering the DEHP content in growing vegetables with Microbacterium sp.J-1. In particular, flowering cabbage is planted in the DEHP contaminated soil, and a Microbacterium sp.J-1 strain is inoculated around a root system of the flowering cabbage. If the Microbacterium sp.J-1 strain is only inoculated into the DEHP contaminated soil, a persistent repairing effect cannot be achieved for the reason that the Microbacterium sp.J-1 strain can die with the extension of inoculation time. However, if the Microbacterium sp.J-1 strain is inoculated into the DEHP contaminated soil, and meanwhile the flowering cabbage is planted, the root system environment of the flowering cabbage provides available organic matter for the Microbacterium sp.J-1 strain, the inoculated strain can survive in the soil for a long time, and the persistent repairing effect on the contaminated soil can be achieved. Meanwhile, the flowering cabbage with the quality safety up to the standard can be planted, and the mode of 'repairing while production' is realized.

Description

technical field [0001] The invention relates to the technical field of biological treatment of environmental pollutants, in particular to a method for using microbacterium J-1 to restore DEHP-contaminated soil and reduce DEHP content in planted vegetables. Background technique [0002] With the rapid development of social economy and urbanization, the unreasonable discharge of a large number of "three wastes" has led to increasingly serious pollution of organic pollutants in farmland soil, posing a serious threat to the quality and safety of agricultural products. Among them, phthalates (PAEs) are the most toxic organic pollutants in farmland soil, and the content is in the order of μg / kg to mg / kg, which is much higher than that of persistent organic pollutants such as polycyclic aromatic hydrocarbons and polychlorinated biphenyls. thing. PAEs are mainly used as plasticizers in the plastics industry, and have become one of the most common pollutants in the world. They are l...

Claims

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Application Information

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IPC IPC(8): B09C1/00B09C1/10A01B79/02C12N1/20C12R1/01
CPCA01B79/02B09C1/10B09C1/105B09C2101/00C12N1/20
Inventor 莫测辉赵海明黄献培蔡全英李彦文李慧胡瑞文黄何彪杜欢
Owner JINAN UNIVERSITY
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