Novel probiotic strain and application thereof

A technology of bacteria strains and supplies, applied in the field of Bacillus subtilis strains, can solve the problems that products cannot be stored for a long time, lactic acid bacteria are not resistant to high temperature, and finished products cannot be sterilized by high temperature, so as to reduce production costs and improve the balance

Active Publication Date: 2011-09-21
林秀霞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Furthermore, the conditions for the cultivation of lactic acid bacteria must be strictly controlled, and the lactic acid bacteria must grow well under a specific temperature, and the lactic acid bacteria are not resistant to high temperature, and the finished product cannot be sterilized by high temperature, so that the product cannot be stored for a long time, and it is easy to breed miscellaneous bacteria

Method used

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  • Novel probiotic strain and application thereof
  • Novel probiotic strain and application thereof
  • Novel probiotic strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: the fermentation culture of probiotic BS139

[0057] In the process of fermentation and cultivation, the key steps include: (1) Screening of probiotics: during fermentation, carbon source: glucose (Glucose), nitrogen source: soybean protein and NaCl and other biochemical reagents are used as bacterial strains for fermentation, and they are placed in centrifuge tubes and shake flask treatment; (2) aerobic spore-producing probiotic culture: put into a 5L tank for culture, and then put into a 100L tank for culture, and then the culture solution is left standing without oxygen supply for 2 days to increase the microbial sporulation rate; (3) ) Advantageous growth screening technology: When cultivating probiotics BS 139, take the bacterial solution every hour, record its growth efficiency, calculate the time point of the highest growth efficiency, and reach the mid-log phase (mid-log phase) in about 6 hours .

Embodiment 2

[0058] Example 2: Probiotic BS139 spore temperature resistance, yield improvement technology

[0059] In terms of improving the temperature resistance and productivity of spores, the dissolved oxygen factor is used to control: a large amount of aeration is used to increase the dissolved oxygen to supply the oxygen demand of the bacteria in the culture medium. If the dissolved oxygen of the culture medium is reduced, the environmental changes will make the microbial production Sporulation, can improve sporulation rate, and utilize culture medium concentration control: utilize sterile water to dilute culture fluid concentration with volume ratio, utilize concentration control to obtain the optimum culture medium concentration of highest sporulation rate (take diluted culture fluid as original concentration 50% and 30%), the spore yield was measured at different concentrations. Through different tests, it is found that the above technologies can improve the sporulation rate, spor...

Embodiment 3

[0060] Embodiment 3: the tolerance test of probiotic BS139 to acid

[0061] Pre-preparation of samples

[0062] First, soak a little bacteria powder in the culture medium, activate it for four hours, apply it on the culture medium and place it in a 37°C incubator for overnight culture (overnight), and do the same with enterohaemorrhagic Escherichia coli (EHEC) preparation.

[0063] After completing sample preparation, follow the steps below:

[0064] 1. Use a toothpick to pick up a little colony on the flat plate and add it to the 6ml culture medium in a centrifuge tube, and place it in a 37°C incubator for 16 hours of rotation.

[0065] 2. Divide the cultured bacterial solution into sterile round bottom culture tubes, namely 1-0, 1-1, 1-2, 1-3 and 1-1s, 1-2s, 1-3s, a total of 7 tubes, 0.8ml per tube.

[0066] 3. Centrifuge the above 7 tubes at 7000rpm for 2min, discard the supernatant, and then quickly centrifuge at 7000rpm for 10sec, then discard the supernatant and leav...

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Abstract

The combination of probiotics and food is helpful for inhibiting the growth of pathogens and absorbing special nutritions. However, the probiotics need to survive at high temperature in the process of food processing, and after the probiotics enter the body, the probiotics must go through the test of stomach acid and bile salt to reach the intestines. Accordingly, the invention provides a bacillus subtilis strain BS139, which is characterized by the survivability of resisting environmental stresses, including the characteristics such as acid resistant, bile salt resistant, high temperature resistance and the like. The probiotic strain is preserved in Agricultural Research Service, NRRL and has a preservation number of NRRL No.B-50347, with further applications in the preparation of food commodities, pharmaceutical compositions, and daily necessities.

Description

technical field [0001] The present invention relates to a novel probiotic bacterium (BS139), in particular to a strain of Bacillus subtilis with tolerance against environmental stress. Background technique [0002] In the human intestinal tract, there are more than 400 types of bacteria (intestinal bacteria) with a total of about 100 trillion, of which 80-85% are normal flora and 15-20% are harmful bacteria. Therefore, it is very important to maintain the ecological balance of the flora. Its functions include helping the decomposition and digestion of food residues, and producing vitamin B group and K. Probiotics produce lactic acid and acetic acid, which can keep the intestinal tract slightly acidic, so that harmful bacteria cannot survive. However, due to the abundance of material, the diet of modern people is becoming more and more refined and particular, coupled with the increase of age, the bacteria in the intestines have undergone great changes, the beneficial bacteria...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L1/30A23K1/16A61K35/74A61K8/99C05F11/08C12R1/125A23K10/18A23L33/135A61K35/742
Inventor 林秀霞游政颖
Owner 林秀霞
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