The invention discloses a bacillus megaterium living bacteria agent, a liquid fermentation process and application thereof. The bacillus megaterus living bacteria agent is prepared by adding bacillusmegaterium into a culture medium and carrying out liquid fermentation, wherein the preservation number of the bacillus megaterium is GMCC No:1259, and the culture medium comprises, 0.5% of sucrose, 0.8% of corn starch, 3% of soybean cake powder, 0.8% of corn steep powder, 0.5% of peptone, 0.05% of calcium carbonate, 0.08% of dipotassium hydrogen phosphate, 0.13% of magnesium sulfate, 0.1% of sodium chloride, 0.07% of zinc sulfate and 0.01% of potassium nitrate. According to the bacillus megaterium living bacteria agent, the liquid fermentation process and the application thereof, the production efficiency of the bacillus megaterium 1259 is greatly improved, the yield is increased, and a method is simple. In dairy cow production, the addition of the bacillus megaterium BM1259 in daily ration can significantly improve the production performance of dairy cows, promote fermentation in rumens and reduce the loss of nitrogen in excreta.