44results about How to "High sporulation rate" patented technology

The invention discloses a bacillus subtilis preparation and preparation method thereof. The bacillus subtilis preparation is prepared by bacillus subtilis fermentation broth prepared by the following steps: accessing bacillus subtilis seed liquid into fermentation medium with 0.5 to 5% (weight ratio) of inoculation amount, and conducting fermentation for 20 to 40 hours, with pressure being 0.02 to 0.15 MPa, agitation speed being 100 to 300 r / m, and ventilation ratio being 1:0.3-1.5(volume ratio). The advantages of the invention: the invention adopts liquid deep fermentation method instead of solid fermentation method to improve efficiency of preparation technology, and further optimizes composition of the mediums used in various steps and fermentation technological conditions to substantially increase live bacteria content of the bacillus subtilis in final product; the bacillus subtilis preparation is acid resistant and cholate resistant, and keeps high efficiency and stability in intestinal tract environment; and gemma formation rate is high, the bacillus subtilis is more high-temperature resistant and convenient for processing and preservation.

The invention provides a composite bacillus preparation containing three strains, a preparation method of the composite bacillus preparation and application of the composite bacillus preparation to ecological breeding, and belongs to the technical field of microorganism and feed additives. Bacillus coagulans, butyric acid clostridia and bacillus megaterium are used as fermentative strains respectively, and large-scale, low-cost and high-density fermentation production of spores is conducted through step-by-step magnification; three live bacterium preparations are prepared by mixing bacterial sludge with the spores and dry starch; the three live bacterium preparations are matched according to the equal weight ratio, the composite bacillus preparation is obtained, and the concentration of the spores reaches more than 1*108-109 cfu / g; the composite bacillus preparation serves as a microorganism and feed additive to be added to farmed animal feed according to the mass ratio ranging from 0.02% to 0.5%. The composite bacillus preparation is simple and stable in process, low in cost, high in spore content and environment tolerance, easy to store and high in survival rate; the purposes of composition and synergism are achieved through the collaborative supplementary effects of different bacilli in the aspect of micro-ecology adjustment, growth of farmed animals is promoted, the feed utilization rate and feed rewards are increased, diarrhea is prevented and reduced, and the breeding ecological environment is improved.

The combination of probiotics and food is helpful for inhibiting the growth of pathogens and absorbing special nutritions. However, the probiotics need to survive at high temperature in the process of food processing, and after the probiotics enter the body, the probiotics must go through the test of stomach acid and bile salt to reach the intestines. Accordingly, the invention provides a bacillus subtilis strain BS139, which is characterized by the survivability of resisting environmental stresses, including the characteristics such as acid resistant, bile salt resistant, high temperature resistance and the like. The probiotic strain is preserved in Agricultural Research Service, NRRL and has a preservation number of NRRL No.B-50347, with further applications in the preparation of food commodities, pharmaceutical compositions, and daily necessities.

The invention discloses an application of a chloramine B solution in chicken coccidiosis oocyst culture and preservation. According to the invention, study finds out that the sporulation rate of the chicken coccidiosis oocysts can be effectively improved with the chloramine B aqueous solution as a chicken coccidiosis oocyst culture solution. The oocysts of the chicken eimeria tenella, eimeria acevulina, eimeria maxima and eimeria necatrix are not affected in activity if being preserved within 6 months; if being preserved within 8 months, the yield of the oocysts is reduced within 25%; the pathogenicity is not obviously affected. Therefore, the chloramine B aqueous solution can be used as the culture and preservation solution for the chicken coccidiosis oocysts in stead of potassium dichromate.

The invention provides a streptomycesvinaceus-drappus microbial inoculum. The streptomycesvinaceus-drappus microbial inoculum uses an active bacteria culture prepared by liquid submerged fermentation or solid fermentation of an active strain of streptomycesvinaceus-drappus, of which the storage number is CGMCC No. 2664, as an active ingredient. A tentative name of the streptomycesvinaceus-drappus microbial inoculum is S506, and the streptomycesvinaceus-drappus microbial inoculum was submitted to the CGMCC for storage on 11st September, 2008. The preparation method comprises the following steps of: A, activation of the strain; B, preparation of liquid seeds of the streptomycesvinaceus-drappus; and C, liquid submerged fermentation of the streptomycesvinaceus-drappus. The invention also provides a novel process of 'liquid seed preparation and solid fermentation' which is favorable for the growth of the streptomycesvinaceus-drappus, and the novel process meets the technical requirements on high effective viable count, high spore production rate, light contamination of crossbred bacteria and short fermentation period. When the active streptomycesvinaceus-drappus is applied to the roots of vegetables, various root diseases, such as blight, root rot and root knot nematode disease, of vegetables can be effectively prevented and controlled, and the streptomycesvinaceus-drappus microbial inoculum has obvious effects of enforcing growth of root systems and degrading autopoisonous matters of root systems, so that the streptomycesvinaceus-drappus microbial inoculum has a wide application prospect in effectively solving the problem of continuous cropping obstacles which are commonly happed to vegetables, fruit trees, medicinal materials and the like.

The invention belongs to the field of veterinary parasites, and relates to coccidian oocyst culture preservative fluid and an application method thereof. The invention is characterized in that the culture preservative fluid takes polyhaxemethylenguanidine hydrochloride as a main ingredient. The application method of the coccidian oocyst culture preservative fluid comprises the following steps of: separating and purifying fresh coccidian oocysts from fowl dung or intestinal tract tissues containing the coccidian oocysts; adding an appropriate amount of a polyhaxemethylenguanidine hydrochloride solution; adjusting the density of the oocysts; controlling the depth of a culture medium; putting the culture medium in a shaking table to perform shaking culture; performing microscopic examination; stopping culture when the sporulation rate of the oocysts exceeds 85%; collecting the oocysts; adding an appropriate amount of the polyhaxemethylenguanidine hydrochloride solution again; and preserving in a refrigerator of which the temperature is 4 DEG C. The coccidian oocyst culture preservative fluid is efficient, long-acting and safe, pollution-free to the environment, simple to prepare and low in cost, and is ideal coccidian oocyst culture preservative fluid. By applying the application method provided by the invention, the operation is simple and convenient, the efficiency is high, the automation can be realized, and the coccidian oocyst culture preservative fluid is suitable for large-scale culture preservation of coccidian oocysts.

The invention provides a production method for bacillus licheniformis with a high sporation rate. According to the production method, no manganese chloride is added into a fermentation medium in a fermentation tank, a sterilized manganese chloride solution is supplemented after fermentation is carried out for 23-25 h, and the volume of the sterilized manganese chloride solution accounts for 0.03-0.04 % of the fermentation volume. As manganese chloride is added in the middle and later stage of fermentation, early-stage growth of the bacillus licheniformis is not affected, the sporation rate of the bacillus licheniformis is increased, the fermentation cycle is shortened, the fermentation production cost is reduced, and better product quality is achieved.

The invention discloses application of lignocellulose hydrolysate for increasing the spore forming rate of bacillus coagulans and / or the spore concentration, and a method for producing bacillus coagulans by utilizing the lignocellulose hydrolysate. Particularly, agriculture and forestry biomass raw materials are hydrolyzed at 105-130 DEG C by utilizing 1-4% of H2SO4; after being detoxified through Ca(OH)2, the obtained lignocellulose hydrolysate as a carbon source is added into a bacillus coagulans fermentation culture medium according to a certain proportion; therefore, the spore forming rate and the spore concentration can be obviously increased; the spore concentration can be up to 2.5*10<11> cfu / mL maximally; and the spore concentration is 5-10 times of that at home and abroad currently. The spore yield of probiotics, namely bacillus coagulans, can be obviously increased by utilizing agriculture and forestry lignocellulose raw materials; raw materials are easy to obtain and low in cost; the production process is simple; and the method has better popularization and application value.

The invention provides a method for promoting formation of bacillus coagulans spores and a microecological preparation and application thereof, and relates to the technical field of micro-ecological preparations. The method comprises that an induction liquid is added in the fermentation culture process, and the induction liquid is bacillus coagulans spore liquid. The problem that the concentrationof viable bacteria and the sporulation rate are difficult to consider at the same time often exists, according to the method, bacillus coagulans spore liquid is creatively added in the fermentation culture process to serve as induction liquid for fermentation culture, so that the spore production rate of bacillus coagulans is effectively increased, the spore forming time is shortened, the production efficiency is greatly improved, and the production cost is reduced.

The invention discloses a method for removing nitrite nitrogen from aquaculture water through aerobic denitrification. The method comprises the following steps that bacillus liquid is fed into aquaculture water; the concentration of the bacillus liquid is 10 <8>to 10<10> bacteria / ml, and the dose per mu of the bacillus liquid is no less than 10kg; moreover, the oxygen dissolution capacity of the aquaculture water is between 2 and 10mg / L; and the temperature is controlled between 13 and 42 DEG C, and the pH is controlled between 5.0 and 9.0. The method has the advantages that the method takes bacillus which still has strong denitrification capability under aerobic condition as original strain; only 10kg of the bacillus liquid needs to be fed into every mu of aquaculture water, and the removal rate of the nitrite nitrogen can reach 92.7 percent after 5 days; the final product after the nitrite nitrogen is removed is N2, and no N2O which can cause environmental pollution is generated; meanwhile, the method has the advantages of simple operation and low cost, and can obtain ideal effects in smaller dose; moreover, the bacillus liquid can also promote the growth of cultured organism, and has double efficacies; therefore, the bacillus liquid is worthy of popularization and application in aquiculture.

The invention discloses a bacillus megaterium living bacteria agent, a liquid fermentation process and application thereof. The bacillus megaterus living bacteria agent is prepared by adding bacillusmegaterium into a culture medium and carrying out liquid fermentation, wherein the preservation number of the bacillus megaterium is GMCC No:1259, and the culture medium comprises, 0.5% of sucrose, 0.8% of corn starch, 3% of soybean cake powder, 0.8% of corn steep powder, 0.5% of peptone, 0.05% of calcium carbonate, 0.08% of dipotassium hydrogen phosphate, 0.13% of magnesium sulfate, 0.1% of sodium chloride, 0.07% of zinc sulfate and 0.01% of potassium nitrate. According to the bacillus megaterium living bacteria agent, the liquid fermentation process and the application thereof, the production efficiency of the bacillus megaterium 1259 is greatly improved, the yield is increased, and a method is simple. In dairy cow production, the addition of the bacillus megaterium BM1259 in daily ration can significantly improve the production performance of dairy cows, promote fermentation in rumens and reduce the loss of nitrogen in excreta.

The invention discloses a bacillus subtilis growth acceleration and endospore generation culture method and aims to solve the problems of low viable count, long fermentation period, low endospore generation rate, difficulty in control of endospore generation related process conditions in a liquid fermentation process of bacillus subtilis. The method includes: activating strains; performing liquid fermentation in shake flasks for seed culture, carrying out fermentation cultivation until residual sugar content of the strains is 0.1%, adding supplementary liquid to control the residual sugar content to range from 0.1 to 0.2, fermenting, supplementing for 5-7h, stopping supplementation and continuing to ferment for 4h, adjusting the pH value to range from 8.5 to 9 and controlling dissolved oxygen to be more than 90% until fermentation is finished. A fermentation medium is composed of glucose, yeast extract, sodium chloride, K2HPO4, MgSO4, MnSO4 and water, and the initial pH value is 7.2-7.5; the supplementary liquid is composed of glucose and yeast extract, and the initial pH value is 7.2-7.5. The bacillus subtilis growth acceleration and endospore generation culture method is used for bacillus subtilis culture.

The invention belongs to the technical field of microorganism strain fermentation, in particular relates to a method for high-density fermentation of a bacillus subtilis strain, and further relates toapplication of the strain to feed additives and agricultural planting. The method for high-density fermentation of the bacillus subtilis strain comprises the following steps: (1) activating a strainof bacillus subtilis; (2) preparing a bacillus subtilis seed liquid; and (3) performing tank fermentation. The method has the beneficial effects that (1) the high-density liquid fermentation method ofthe bacillus subtilis has a final fermentation level of 40 billion / ml or greater; and (2) the method is short in fermentation time, that is, about 18-24 hours, the production cost can be reduced, theefficiency can be improved, the fermentation level is high, and the spore formation rate is high.

[PROBLEMS] To provide a seed koji for brewing, which is suitable for wheat allergy patients and wheat intolerance patients (patients with celiac disease) and the medium of which is completely free from wheat-origin component, a koji for brewing, brewed foods and a method of producing the same. [MEANS FOR SOLVING PROBLEMS] Whole soybeans are hulled. A koji mold belonging to the genus Aspergillus is inoculated into the soybean hull thus obtained and cultured therein to give a solid seed koji. By using this seed koji, brewed foods (for example, soy sauce, miso, a liquid seasoning and so on) free from wheat-origin component are produced. Thus, it is possible to obtain a seed koji which is free from wheat-origin component, shows a high sporulation rate of the koji mold and has excellent koji-making properties. Use of this seed koji makes it possible to produce brewed foods which are comparable in qualities to the brewed foods produced by using a seed koji which has been cultured in a wheat medium.

The invention belongs to the field of biotechnology, a method for promote sporulation of cells of Bacillus coagulans by use lactate and / or lactic acid as a sole or main carbon source for growth of that bacillus coagulans comprises culturing the bacillus coagulans in a culture medium consist of yeast extract 10.0 g / L, aerobically culturing the bacillus coagulans in a culture medium consisting of yeast extract 10.0 g / L, and culturing the bacillus coagulans in an aerobic culture medium containing yeast extract 10.0 g / L. Beef extract 10.0 g / L; Peptone 10.0 g / L; 1.5 g / L sodium bicarbonate; 1.5 g / Lpotassium dihydrogen phosphate; 1.0 g / L magnesium sulfate heptahydrate; 0.1 g / L manganese sulfate; 1.0 g / L calcium chloride; 1L of deionize water; Lactate and / or lactic acid content of 10-30g / L. Bacillus coagulans cell can be promoted to form spore in that process of culturing Bacillus coagulans, and the spore formation rate is high than 90%.

The invention discloses a solid fermentation method of bacillus coagulans. The method comprises the following steps of: (1) putting the bacillus coagulans in a seed culture solution for recovery passage so as to obtain a seed bacterium solution; (2) inoculating the prepared seed bacterium solution in a sterile solid culture medium for fermental cultivation so as to obtain zymophyte; and (3) drying the prepared fermentation products at 60 DEG C in vacuum so as to prepare finished fermentation bacillus coagulans product. The solid fermentation method of bacillus coagulans disclosed by the invention has the beneficial effects that the fermentation time is 24 hours and is saved, the fermentation period is shortened, besides, the cell concentration is large, spore formation rate is high, common fermentation matrix is used, so the storage and transportation are convenient; not only are no waste liquid and environmental pollution caused, but also the cost is low.

The invention discloses a Bacillus coagulans strain and application thereof. A preservation number of the Bacillus coagulans strain is CGMCC No.17374. The Bacillus coagulans strain is applied to microbial preparation field. The screened strain has the characteristics of highly producing spores and acid; the strain has obvious difference with other congeneric strains, formation rate of the spores is very high, the heat resistance is good, and the fermentation level is high; the strain is a probiotic and has the effects of maintaining balance of intestinal tracts, stimulating the immune and improving the health level of bodies same as normal lactic acid bacteria. A capsule preparation prepared by taking the strain as a core component has a good treatment effect and a function of regulating multiple intestinal discomforts.

The invention relates to a method for preparing a composite biological fertilizer from tremellose bacillus and gigantochromoblast, which comprises: culturing and growing secondary tremellose bacillus strain and secondary gigantochromoblast strain; then, (1) stirring the solid culture medium uniformly, and placing into a disinfected solid fermentation pool, wherein, the weight percentage of the components are: wheat bran 15-25%, straw 25-36%, fly ash 11-19%, bean cake powder 3-9%, CaCO3 0.3-0.9%, turf 7.5-14%, K2HPO4 0.1-0.6%, sucrose 1-5%, and fertilizer soil 9-15%; (2) inoculating the two secondary strains into the solid fermentation pool according to that: the inoculation amount is 20% of the total weight, while the amount of the secondary tremellose bacillus strain is twice of the secondary gigantochromoblast strain; (3) adding water into the solid fermentation pool to keep the humidity of the ferment material at 50%, sealing and controlling the temperature in the solid fermentation pool, and fermenting and growing for 48-54 hours to obtain the finished product. The invention adopts combined solid fermentation of double bacterium, accelerates the strain breeding speed, shortens the fermentation period, reduces production cost, and improves the blastema formation ratio of the product.

The invention discloses bacillus subtilis and a fermentation method with high yield of indoleacetic acid and high spore formation rate, and belongs to the technical field of microbial fermentation. The bacillus subtilis is named as 8-32, is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 25, 2020, and has a preservation number of CGMCC NO.20824. The invention further discloses a preparation method of the bacillus subtilis. According to the liquid bacillus subtilis strain fermentation method, the content of bacillus subtilis reaches 2*10<10> CFU / mL or above, the content of indoleacetic acid reaches 120 mg / L or above, the spore formation rate reaches 98% or above through adoption of a method of supplementing materials, controlling fermentation conditions and treating fermentation liquor after fermentation, the high yield of indoleacetic acid can be achieved, and the spore formation rate is high. The problems of low yield of indoleacetic acid, long spore formation induction time and unstable spore formation rate in liquid fermentation are solved.

The invention discloses a method for recovering and using waste fermentation broth of paenibacillus polymyxa. The method comprises following steps: (1) adding 0.05 to 0.1 wt% of chitosan into centrifugally obtained waste fermentation broth of paenibacillus polymyxa, stirring to carry out flocculation, carrying out centrifugation, and saving the supernate; (2) adding 0.001 to 0.005 wt% of amylase into supernate, and carrying out enzymatic hydrolysis for 15 to 60 minutes; and (3) after enzymatic hydrolysis, making the supernate go through a cation exchange column, and adding the obtained producttaken as a fermentation synergist into a bacillus fermentation culture medium. The synergist can reduce the using amount of the bacillus fermentation culture medium and increase the forming rate of spores.

The invention relates to a chicken coccidial oocyst protective solution and a preparation method thereof. The composition consists of the following components in parts by weight: 5-10 parts of chlorhexidine acetate, 10-40 parts of sodium benzoate, 5-10 parts of sodium chloride, 10-80 parts of sterilized water and 10-30 parts of [beta]-mannase. The protective solution provided by the invention is scientific and reasonable in formula composition, simple in process, convenient to use and relatively low in cost; the protective solution, which takes such ingredients as the chlorhexidine acetate and the like as chicken coccidial oocyst protective liquid, can effectively improve a sporation rate of chicken coccidial oocysts; and when chicken E.tenella, E.acervulina, E.maxima and E.necatrix coccidial oocysts are preserved within 6 months, activity is free from any influence, and the chicken E.tenella, E.acervulina, E.maxima and E.necatrix coccidial oocysts, when preserved within 8 months, conform to requirements based upon safety test and efficacy test.

The invention provides a soil conditioner containing potassium bacteria and a preparation method of the soil conditioner. The soil conditioner contains the potassium bacteria which can convert solidified ineffective potassium in the soil into quick-acting potassium, so that the problem that crops lack potassium is solved, and damage to environment due to use of chemical fertilizer is reduced; meanwhile, a fermentation method different from tradition methods is adopted, radix astragali powder and hydrogen peroxide are added during fermentation and can stimulate the potassium bacteria to produce spores and enhancing the vitality of the potassium bacteria, thus, the viable count and the spore formation rate of the potassium bacteria are increased, and the activity and efficiency of the soil conditioner are guaranteed.

The invention relates to the technical field of insect biological control, in particular to a locust microsporidia AL200801 strain as well as a biological preparation and application of the locust microsporidia AL200801 strain. The invention provides a locust microsporidia AL200801 strain, and the preservation number of the locust microsporidia AL200801 strain is CCTCC No. V202137. The locust microsporidia AL200801 strain disclosed by the invention has the advantages of high timeliness, high lethality, high sporulation rate, no toxicity and strong obligate property.

The invention discloses a fruit and vegetable natto fermentation technology which includes the steps: (1) soaking natto; (2) preparing natto companions A; (3) weighing the natto companions A accordingto 5% w / w of the soaked natto, adding 4 times of concentrated potato pulp w / v and uniformly coating the surface of the soaked natto with the concentrated potato pulp after homogenization; (4) cookingthe natto obtained in the step 3 at high temperature; (5) culturing strains; (6) performing inoculation and fermentation; (7) preparing natto companions B; (8) adding the natto companions B accordingto the proportion of 12% w / w of the fermented natto in the step 6, uniformly coating the surface of the natto with the natto companions B and performing baking fermentation until drying; (9) crushingthe dry natto in a clean environment and then placing the dry natto into a capsule or tablet to obtain a finished product. According to the fruit and vegetable natto fermentation technology, bacillusnatto grows vigorously, is high in spore forming rate, has fruit and vegetable fermentation flavor and is suitable for Chinese people.

The invention discloses a method for promoting aspergillus flavus to produce spores rapidly. The method comprises the following steps: taking a graphene doped solid culture medium as a culture mediumand inducing mycelium of the aspergillus flavus to produce the spores rapidly. The method has the following advantages: the spore-producing experimental period of the mycelium of the aspergillus flavus is shortened; and the mycelium of the aspergillus flavus can be induced rapidly to produce a large amount of spores within 48 to 72 hours. Good basis is laid for later research of the aspergillus flavus; a large amount of precious time is saved for scientific research workers; and high application potential is achieved.

The invention discloses a bacillus subtilis preparation and preparation method thereof. The bacillus subtilis preparation is prepared by bacillus subtilis fermentation broth prepared by the following steps: accessing bacillus subtilis seed liquid into fermentation medium with 0.5 to 5% (weight ratio) of inoculation amount, and conducting fermentation for 20 to 40 hours, with pressure being 0.02 to 0.15 MPa, agitation speed being 100 to 300 r / m, and ventilation ratio being 1:0.3-1.5(volume ratio). The advantages of the invention: the invention adopts liquid deep fermentation method instead of solid fermentation method to improve efficiency of preparation technology, and further optimizes composition of the mediums used in various steps and fermentation technological conditions to substantially increase live bacteria content of the bacillus subtilis in final product; the bacillus subtilis preparation is acid resistant and cholate resistant, and keeps high efficiency and stability in intestinal tract environment; and gemma formation rate is high, the bacillus subtilis is more high-temperature resistant and convenient for processing and preservation.

The invention provides a low-cost, environment-friendly method for producing phosphorus bacteria biological fertilizer. The phosphorus bacteria biological fertilizer uses Bacillus megaterium as a production bacteria and sewage sludge as a production matrix and is produced through fermentation culture in seed tanks and fermentation tanks. The fermentation period of the whole growth process is shortened to 36-40 hours and bacillus formation rate is increased by 20%-40% compared with common methods and the live bacteria number in the fermentation liquid can reach 0.8-1 billion / ml and can reach 0.15-0.2 billion / g when the solution is absorbed with dry sterile peat. Moreover, the contents of cadmium, mercury, lead, chromium, arsenic and other heavy metals in the fermentation phosphorus bacteria fertilizer are much lower than the contents before fermentation. The pepper field experimental results show that the phosphorus bacteria biological fertilizer can increase the fertilizer utilizationand output and benefit, and has positive effect to the agronomic traits such as the height of crops, width, weight, uniformity, the number of branches and the first harvest time, and meanwhile the comprehensive disease resistance is further enhanced.

The invention relates to a paecilomyces lilacinus strain PNVT8 for expressing the hemoglobin VHb of vitreoscilla and the application of the paecilomyces lilacinus strain PNVT8. The recombinant paecilomyces lilacinus strain PNVT8 is collected in the China center for type culture collection with the collection number of CCTCC No:M 2013296. The paecilomyces lilacinus strain PNVT8 is obtained by optimizing a codon, a promoter and a gene structure, successfully integrating the hemoglobin VHb from the vitreoscilla into a chromosome of a wild paecilomyces lilacinus strain ACSS which has a plant parasitic nematode killing effect and is provided by the agricultural culture collection of China, and performing screening. Compared with the wild paecilomyces lilacinus strain ACSS, the paecilomyces lilacinus strain PNVT8 has the advantages that more extracellular proteases, chitinase, alpha-amylases and the like can be generated, and meanwhile, the biomass and the cell yield are improved; after the paecilomyces lilacinus strain PNVT8 is applied as a product, the growth rate during field planting under a soil micro-aerobic condition also is increased to further improve the nematode larva killing activity.