Bacillus subtilis growth acceleration and endospore generation culture method

Abstract

The invention discloses a bacillus subtilis growth acceleration and endospore generation culture method and aims to solve the problems of low viable count, long fermentation period, low endospore generation rate, difficulty in control of endospore generation related process conditions in a liquid fermentation process of bacillus subtilis. The method includes: activating strains; performing liquid fermentation in shake flasks for seed culture, carrying out fermentation cultivation until residual sugar content of the strains is 0.1%, adding supplementary liquid to control the residual sugar content to range from 0.1 to 0.2, fermenting, supplementing for 5-7h, stopping supplementation and continuing to ferment for 4h, adjusting the pH value to range from 8.5 to 9 and controlling dissolved oxygen to be more than 90% until fermentation is finished. A fermentation medium is composed of glucose, yeast extract, sodium chloride, K2HPO4, MgSO4, MnSO4 and water, and the initial pH value is 7.2-7.5; the supplementary liquid is composed of glucose and yeast extract, and the initial pH value is 7.2-7.5. The bacillus subtilis growth acceleration and endospore generation culture method is used for bacillus subtilis culture.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation; in particular, it relates to a cultivation method for promoting the growth of Bacillus subtilis and producing spores. Background technique [0002] Bacillus subtilis is an aerobic bacterium and an important industrial strain. It has been widely used in animal husbandry, medicine, plant disease control and other fields. It has broad application prospects and shows huge social and ecological benefits. . Most products of Bacillus subtilis microecological preparations use live bacteria to function. The effective number of viable bacteria is an important indicator to measure the quality of microecological preparations. The product must contain a considerable number of viable bacteria to function. However, with the prolongation of storage time, the number of viable bacteria in probiotics decreases continuously, and within a certain period of time, the decrease in the number of effective...

AI Technical Summary

Problems solved by technology

[0004] The invention solves the problems that the process conditions related to spore formation are not easy to control, the spore formation rate is low, the fermentation period is long, and the number of viable bacteria is low in the liquid fermentation process of Bacillus subtilis, and the high-density cultivation of Bacillus subtilis can be achieved while obtaining more than 95% sporulation rate; provides a culture method to promote the growth of Bacillus subtilis and produce spores