Bacillus subtilis growth acceleration and endospore generation culture method
A Bacillus subtilis, culture method technology, applied in the field of promoting the growth of Bacillus subtilis and producing spores, can solve the problems of low spore formation rate, low number of viable bacteria, long fermentation period, etc., and achieve extended storage period and high density Cultivation improves and increases the effect of stabilizing activity
Inactive Publication Date: 2015-09-09
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
1 Cites 7 Cited by
AI-Extracted Technical Summary
Problems solved by technology
View more
Abstract
The invention discloses a bacillus subtilis growth acceleration and endospore generation culture method and aims to solve the problems of low viable count, long fermentation period, low endospore generation rate, difficulty in control of endospore generation related process conditions in a liquid fermentation process of bacillus subtilis. The method includes: activating strains; performing liquid fermentation in shake flasks for seed culture, carrying out fermentation cultivation until residual sugar content of the strains is 0.1%, adding supplementary liquid to control the residual sugar content to range from 0.1 to 0.2, fermenting, supplementing for 5-7h, stopping supplementation and continuing to ferment for 4h, adjusting the pH value to range from 8.5 to 9 and controlling dissolved oxygen to be more than 90% until fermentation is finished. A fermentation medium is composed of glucose, yeast extract, sodium chloride, K2HPO4, MgSO4, MnSO4 and water, and the initial pH value is 7.2-7.5; the supplementary liquid is composed of glucose and yeast extract, and the initial pH value is 7.2-7.5. The bacillus subtilis growth acceleration and endospore generation culture method is used for bacillus subtilis culture.
Application Domain
BacteriaMicroorganism based processes
Technology Topic
GROWTH ACCELERATIONSChloride sodium +10
Examples
- Experimental program(1)
Example Embodiment
PUM


Description & Claims & Application Information
We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.
Similar technology patents
Keep-alive conveying and seed preservation method of eucheuma denticulatum
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Application of plant lactobacillus to preservation of bacillus subtilis solid inoculant and method for extending preservation period of bacillus subtilis
Owner:COFCO BIOTECHNOLOGY CO LTD +1
Sweet osmanthus flavor sticky rice filled lotus root and preparation method thereof
Owner:ANHUI THREE SQUIRRELS ELECTRONICS BUSINESS
Classification and recommendation of technical efficacy words
- long shelf life
Devices and methods for dispensing reagents into samples
Owner:3M INNOVATIVE PROPERTIES CO
Preparation method for purely-natural yeast powder
Owner:福建安麦高新生物技术有限公司