Engineered extracellular matrix preparation method

A technology of tissue engineering and extracellular matrix, applied in the field of artificial organs of biomaterials, can solve the problems of poor clinical effect, achieve the effect of repairing malnutrition, easy to use, and preventing recurrence

Active Publication Date: 2006-07-12
SHAANXI BOYU REGENERATIVE MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its sources mainly include: human-derived (such as Alloderm in the United States) has good biocompatibility with the body because it is derived from the human body, but it is difficult to be widely used due to limited sources and ethical issues; Substrates (such as porcine acellular dermis) have poor clinical results due to the presence of immune rejection between species

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Now in conjunction with experiment, preparation method of the present invention is described in further detail:

[0047] 1. Preparation of culture medium

[0048] (1) Basal culture medium

[0049] Ingredient Final Concentration

[0050] Commercial minimum essential medium DMEM 67.5% (v / v)

[0051] Commercial culture medium F12 22.5% (v / v)

[0052] Fetal bovine serum 10% (v / v)

[0053] Insulin 10ng / ml

[0054] Hydrocortisone 30ng / ml

[0055] Adenine 0.22mM

[0056] Transferrin 6mg / ml

[0057] Epidermal Growth Factor 80ng / ml

[0058] Antibiotics 180i.u / ml

[0059] (2) Culture medium 1

[0060] Basal Medium + Bovine Pituitary Extract 0.5% (V / V)

[0061] (3) Culture medium 2

[0062] Basal medium + vitamin C 20μg / ml

[0063] (4) Culture medium 3

[0064] Basal medium + basic fibroblast growth factor 5ng / ml

[0065] 2. Extraction of matrix protein: the present invention adopts the matrix protein mixture extracted from the skin of mammals (can be cattle...

Embodiment 2

[0071] Embodiment 2. burn wound repair test

[0072] 1) Preparation of animal skin deep second-degree scald model: 50kg Yorkie pigs were anesthetized, and the back skin was prepared for disinfection and sterile towels were spread. Using a scald instrument: temperature 100°C, pressure 1kg, time 25s; prepare the burn wound ( Figure 4 ).

[0073] group

Number of cases

Average healing time (days)

Blank control group

10

24.37±3.25

Using the extracellular matrix group of the present invention

10

13.65±3.41

[0074] After using the extracellular matrix repair group prepared by the present invention for 2 weeks, it can be seen that the skin defect has been repaired, the scar is not obvious, and the color is close to the normal skin color ( Figure 5 ).

[0075] Conclusion: The tissue engineered extracellular matrix prepared in the present invention can significantly shorten the healing time of scald wound.

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Abstract

The invention relates to a method for preparing for organization project cell epimatrix, which adopts animal stromatin and desmocyte as raw material. It comprises: preparing for the culture liquid, extracting the stromatin, preparing for the cell-stromatin culture material, preparing for the cell epimatrix, dispelling the cell by freezing, and sterilization sanitizing packaging material and so on.

Description

technical field [0001] The invention belongs to the technical field of artificial organs of biological materials, and in particular relates to a preparation method of tissue engineering extracellular matrix. Background technique [0002] The extracellular matrix is ​​secreted and synthesized by cells, located outside the cells, and constitutes connective tissue. It not only has the physical functions of connection, support, water retention, compression resistance and protection, but also plays a full range of biological functions on the life activities of cells, mainly manifested in: (1) Affect the survival, growth and death of cells; most normal eukaryotic cells, except mature blood cells, must adhere to the extracellular matrix to inhibit apoptosis and survive. Once epithelial cells and endothelial cells are separated from the extracellular matrix, they will Programmed death will occur. (2) Determine the shape of the cell; this effect is achieved through its receptors aff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08A61F2/02C12N5/07
Inventor 王平安
Owner SHAANXI BOYU REGENERATIVE MEDICINE CO LTD
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