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Bacillus subtilis preparation and preparation method thereof

A Bacillus subtilis and seed technology, applied in the field of agricultural biology, can solve the problems of not being able to fully and effectively adapt to the external environment, reducing the efficacy, and not having enough viable bacteria.

Active Publication Date: 2013-10-16
北京科为博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing Bacillus subtilis preparations generally have the defect that the number of viable bacteria contained is not high enough. In addition, they have poor stability during transportation, storage and use, and cannot fully and effectively adapt to the external environment and the internal environment of livestock and poultry. These all lead to a reduction in the potency of existing Bacillus subtilis formulations

Method used

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  • Bacillus subtilis preparation and preparation method thereof
  • Bacillus subtilis preparation and preparation method thereof
  • Bacillus subtilis preparation and preparation method thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of Bacillus subtilis liquid preparation-L1

[0042] 1) Primary slant culture of Bacillus subtilis: The Bacillus subtilis with the preservation number ACCC10619 purchased from the China Agricultural Microorganism Culture Collection Management Center was used as the starting strain. Bacillus subtilis ACCC10619 was inoculated on the first-grade slant medium by conventional methods, and cultured at 30°C for 24 hours to obtain a single colony of first-grade Bacillus subtilis; the medium was a mixture prepared according to the following ratio and ingredients: beef soak Cream 5.0g, soybean peptone 15.0g, yeast extract 5.0g, sodium chloride 5.0g, glucose 10.0g, agar 20g, distilled water 1000mL, pH 7.0;

[0043] 2) Secondary liquid culture of Bacillus subtilis: Inoculate the single colony of the first-grade Bacillus subtilis cultivated in step 1) into the secondary culture medium by a conventional method. Cultivate under the condition of dividing for 15 ho...

Embodiment 2

[0046] Example 2 Preparation of Bacillus subtilis liquid preparation-L2

[0047] 1) Primary slant culture of Bacillus subtilis: The Bacillus subtilis with the preservation number ACCC10619 purchased from the China Agricultural Microorganism Culture Collection Management Center was used as the starting strain. Bacillus subtilis ACCC10619 was inoculated on the primary slant medium by conventional methods, and cultured at 28°C for 36 hours to obtain a single colony of primary Bacillus subtilis; the primary slant medium was prepared according to the following components and ratios The mixture: beef extract 15.0g; soybean peptone 15.0g; yeast extract 5.0g; sodium chloride 5.0g; glucose 10.0g; agar 30g; distilled water 1000mL; pH 7.4;

[0048] 2) Secondary liquid culture of Bacillus subtilis: inoculate the single colony of the first-grade Bacillus subtilis cultivated in step 1) into the secondary culture medium by a conventional method, with a liquid volume of 200mL / 500mL, at 28°C a...

Embodiment 3

[0051]Embodiment 3 prepares bacillus subtilis liquid agent-L3

[0052] 1) Primary slant culture of Bacillus subtilis: Bacillus subtilis with the preservation number CGMCC11508 obtained from the China General Microorganism Culture Collection and Management Center was used as the starting strain. Bacillus subtilis CGMCC11508 was inoculated on the primary slant medium by conventional methods, and cultured at 36°C for 20 hours to obtain a single colony of primary Bacillus subtilis; the primary slant medium was prepared according to the following components and ratios The mixture: beef extract 5.0~15.0g, soybean peptone 10.0g, yeast extract 5.0g, sodium chloride 5.0g, glucose 10.0g, agar 25g, distilled water 1000mL, pH 7.5;

[0053] 2) Secondary liquid culture of Bacillus subtilis: inoculate the single colony of the first-grade Bacillus subtilis cultivated in step 1) into the secondary culture medium by a conventional method, with a liquid volume of 150mL / 500mL, at 36°C and 200 rpm...

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Abstract

The invention discloses a bacillus subtilis preparation and preparation method thereof. The bacillus subtilis preparation is prepared by bacillus subtilis fermentation broth prepared by the following steps: accessing bacillus subtilis seed liquid into fermentation medium with 0.5 to 5% (weight ratio) of inoculation amount, and conducting fermentation for 20 to 40 hours, with pressure being 0.02 to 0.15 MPa, agitation speed being 100 to 300 r / m, and ventilation ratio being 1:0.3-1.5(volume ratio). The advantages of the invention: the invention adopts liquid deep fermentation method instead of solid fermentation method to improve efficiency of preparation technology, and further optimizes composition of the mediums used in various steps and fermentation technological conditions to substantially increase live bacteria content of the bacillus subtilis in final product; the bacillus subtilis preparation is acid resistant and cholate resistant, and keeps high efficiency and stability in intestinal tract environment; and gemma formation rate is high, the bacillus subtilis is more high-temperature resistant and convenient for processing and preservation.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a bacillus subtilis preparation and a preparation method thereof. Background technique [0002] The microorganisms in the intestinal tract of livestock and poultry are closely related to the digestion and absorption of feed in the body, which directly affects the anabolism in the body. Keeping the beneficial flora in the intestines normal can prevent livestock and poultry from contracting diseases, resist harmful bacteria, produce lactic acid, hydrogen peroxide and antibacterial substances at the same time, and promote the digestion and absorption of nutrients. [0003] Bacillus subtilis is rod-shaped, with a diameter of 0.6-1.0 μm and a length of 1.5-2.0 μm. The spores are elliptical, the cysts are not enlarged, and the Gram reaction is positive. The colonies are rough, opaque, non-shiny, expanded, stained white or slightly yellowish. In animal husbandry ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/125A23K10/12A23K20/00
Inventor 吴培均姚琨杨忠广李富伟李兆勇
Owner 北京科为博生物科技有限公司
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