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Method for producing probiotics by utilizing lignocellulose hydrolysate

A technology of lignocellulose and hydrolyzate, which is applied in the directions of using microorganisms, spores, and microorganism-based methods, etc., can solve the problems of complicated fermentation process, limited production and application of Bacillus coagulans preparations, low spore formation rate, etc. To solve the problem, to achieve the effect of simple production process, good popularization and application value, and improved spore yield

Active Publication Date: 2015-07-08
SOUTH CHINA NORMAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

The above-mentioned methods can effectively increase the concentration of Bacillus coagulans spores, and the order of magnitude of spore concentration is generally 109~1010 cfu / mL, but it is difficult to increase further. Moreover, the fermentation cycle is relatively long, the formation rate of spores is low, and the main raw materials of the fermentation medium are fine raw materials such as glucose, yeast extract or peptone, the production cost of fermentation is high, and some fermentation processes are more complicated.
All in all, in the current fermentation production of Bacillus coagulans, the conversion rate of spores is not high, the concentration of spores is low, the production process is complicated, and the fermentation medium with high cost is used, all of which limit the large-scale production and application of Bacillus coagulans preparations. break through this bottleneck

Method used

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  • Method for producing probiotics by utilizing lignocellulose hydrolysate
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  • Method for producing probiotics by utilizing lignocellulose hydrolysate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 utilizes lignocellulose hydrolyzate to produce Bacillus coagulans

[0049] 1. Preparation of lignocellulose hydrolyzate

[0050] (1) Grinding agricultural and forestry biomass raw materials (lignocellulosic raw materials: rice straw, corn straw, peanut shells, wood chips, bagasse, walnut shells, rice bran or bran) into powder (particle size <0.05mm) , according to the solid-to-liquid ratio of 10% (g / mL), mix the pulverized agricultural and forestry biomass raw materials with 1-4% sulfuric acid, react at 105-130°C for 1-2 hours, and vacuum filter to obtain the filtrate;

[0051] (2) Using Ca(OH) 2 Detoxify the filtrate: add Ca(OH) to the filtrate obtained in (1) 2 , adjust the pH of the solution to 9.0-10.0, settle for 1 hour and then extract the filtrate by suction filtration;

[0052] (3) with 2M H 2 SO 4 The pH of the filtrate obtained in (2) was adjusted to 6.0, and after standing for 1 h, the filtrate was extracted by suction filtration. The fil...

Embodiment 2

[0064] Embodiment 2 The impact of different agricultural and forestry biomass raw materials on the spore production of Bacillus coagulans

[0065] 1. Different types of agricultural and forestry biomass raw materials (lignocellulosic raw materials) have different chemical compositions, and the types and contents of five-carbon sugars, six-carbon sugars and other chemical components produced after acid hydrolysis are also different. In this study, some common and large quantities of cellulosic lignin raw materials (rice straw, corn straw, peanut shell, sawdust, bagasse, walnut shell, rice bran, bran) were selected and hydrolyzed with 2% dilute sulfuric acid for 1.5h at 121°C , through Ca(OH) 2 Detoxification and other treatments (same as Example 1) to obtain the hydrolyzate.

[0066] 2. Determining the content of reducing sugar and phenolic substances in the hydrolyzate. Reducing sugars were determined by the Fehling method (Wu Guofeng et al. "Industrial Fermentation Analys...

Embodiment 3

[0072] Example 3 Bacillus coagulans sporulation curve

[0073] As described in Example 2, the addition of rice straw acid hydrolyzate can effectively increase the yield of spores. The rice straw acid hydrolyzate was added at a ratio of 20% (v / v), cultivated for 48 hours according to the method of Example 2, and samples were taken at different times , Determination of spore number and sporulation rate.

[0074] The results are attached image 3 Shown, 12 hours sporulation rate reaches 51%, and 24 hours just arrived the peak period of sporulation. The sporulation rate is 95%, and the number of spores is 2×10 11 cfu / mL. The peak of spore production in the culture medium without adding lignin hydrolyzate generally takes about 48 hours. Adding lignin hydrolyzate can significantly shorten the spore-producing time of Bacillus coagulans and increase the formation rate of spores.

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Abstract

The invention discloses application of lignocellulose hydrolysate for increasing the spore forming rate of bacillus coagulans and / or the spore concentration, and a method for producing bacillus coagulans by utilizing the lignocellulose hydrolysate. Particularly, agriculture and forestry biomass raw materials are hydrolyzed at 105-130 DEG C by utilizing 1-4% of H2SO4; after being detoxified through Ca(OH)2, the obtained lignocellulose hydrolysate as a carbon source is added into a bacillus coagulans fermentation culture medium according to a certain proportion; therefore, the spore forming rate and the spore concentration can be obviously increased; the spore concentration can be up to 2.5*10<11> cfu / mL maximally; and the spore concentration is 5-10 times of that at home and abroad currently. The spore yield of probiotics, namely bacillus coagulans, can be obviously increased by utilizing agriculture and forestry lignocellulose raw materials; raw materials are easy to obtain and low in cost; the production process is simple; and the method has better popularization and application value.

Description

technical field [0001] The invention belongs to the technical field of probiotic production. More specifically, it relates to a method for producing probiotics using lignocellulose hydrolyzate. Background technique [0002] The abuse of antibiotics in animal feed causes the emergence of drug-resistant bacteria and the residue of antibiotics, which are a great threat to human health. Since Fleming in England discovered antibiotics in 1929, they have made great contributions to human production and health. Adding antibiotics to feed has played a positive role in preventing animal diseases, promoting animal growth, improving feed conversion rate, and increasing the output of livestock and poultry products. However, the excessive use of feed antibiotics has also caused various disadvantages, such as the destruction of the dynamic balance of the intestinal flora, whether it is a therapeutic dose or a residual dose of antibiotics, it will destroy the inherent colonization of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12N1/22C12N1/20C12R1/07
Inventor 尹亮陈枚晓曾天欢
Owner SOUTH CHINA NORMAL UNIVERSITY
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