33 results about "Blastema" patented technology

The invention relates to a method for cultivating and producing sporocarps of Dictyophora multicolor Berk. and Broome (1882), which is characterized by comprising the following steps: carrying out the tissue isolation on the fresh sporocarps of the wild dictyophora multicolor berk. and broome (1882), inoculating the fresh sporocarps of the wild dictyophora multicolor berk. and broome (1882) to integrated PDA slope culture medium or pine needle decoction culture medium, culturing until a slope is covered by mycelia to obtain a slope maternal strain, inoculating the slope maternal strain to wheat grain culture medium, culturing until the wheat grain culture medium is covered by mycelia, selecting strains with white, thick and thick mycelia as original strains, sowing the original strains in culture medium for producing layer by layer, covering the culture medium for producing by soil, culturing until the mycelia grow out of the surface of the soil covered on the culture medium for producing, forming a large number of rhizomorphs from the mycelia, expanding the tail ends of the rhizomorphs to form young blastemas and buds, continuing culturing until indusiums have the largest opening degree, and harvesting the fresh sporocarps of the dictyophora multicolor berk. and broome (1882). Due to the adoption of the method, the biological efficiency of the sporocarps of the dictyophora multicolor berk. and broome (1882) can be 1.5-2.5kg/m<2>. The sporocarps of the dictyophora multicolor berk. and broome (1882) can be used as medicinal raw materials or research materials.

The invention discloses an Luc-GFP marked high-transitivity human hepatoma cell line. Hepatoma cells are taken as mother cells, a virus solution packaged with pCDH-CMV-Luc-EF1-GFP-Puro is transfectedthrough slow viruses, and then through puromycin screening, the high-transitivity human hepatoma cell line with stable GFP expression and luciferase activity is obtained. By using the Luc-GFP marked high-transitivity human hepatoma cell line, an in situ hepatoma model for a naked mouse is established through a cell suspension method and a tumor tissue block conversion method respectively, the tumor formation rate of the hepatoma model is 100% respectively, the rates of lung metastasis are 100% and 80% respectively, a foundation is laid for monitoring the growth of tumor in the level of livinganimals and evaluating the curative effect of hepatocellular carcinoma metastasis resistant medicine, and the cell line has greater application prospect.

The invention discloses a method for converting the meloidogynosis agrobacterium lead banana gene with the fluid co culture system, which comprises the following steps: stewing the banana ECS in the meloidogynosis agrobacterium and the black condition for a spell of time; proceeding with the co culture in the banana ECS liquid culture medium; transferring the banana ECS to the idiosome directly in order to induce and grow; culturing on the selective culture medium; transferring the mature resistant idiosome to the blastema germination culture medium; culturing in the light-black alternative condition until the idiosome is germinated; getting sprout; transferring the sprout to the basis screen selective culture medium; acquiring the whole conversion plant by culturing in the light-black alternative condition. The invention changes the co culture condition, which solves the brown problem of banana ECS in the co culture process, and shortens the cycle of converting the plant.

The invention belongs to the technical field of sperm cell treatment, and specifically relates to a method for isolating and identifying sperm cells of Buffalo testis, including the following steps: obtaining Buffalo testis, digest with collagenase IV, hyaluronidase and DNase I combined enzyme, sieving, filtering, and taking suspension cells after differential adherence; 2) taking the suspend cells for live cell stain; and 3) separating by flow cytometry. Beneficial effect: the technical scheme is an effective method for separating buffalo sperm cells, By digesting bovine testis tissue with two enzymes, so that a single cell suspension is obtain, the interference of somatic cells is removed by the method of continuous differential adherence, according to the fluorescence intensity of non-adherent cells stained by flow cytometry, the DNA content of cells is judged, and the interference of other non-target cells such as diploid spermatogonia and tetraploid primary meningoblasts is effectively digested and removed to achieve the successful sorting of a large number of haploid spermatozoa.

The invention relates to 'application of a hydrolytic enzyme CwlC in metrocyte lysis of bacillus'. The invention identifies a novel cwlC gene which joins in metrocyte lysis in B.thuringiensis, cwlC gene deletion can completely block metrocyte lysis, and the yield of spore formation and Cry1Ac protein are not affected. Meanwhile, a CwlC protein marked by a GFP (Green Fluorescent Protein) can be tightly combined with cell walls. More importantly, walls of cells of B.thuringiensis and Bacillus cereus with activities in logarithmic phases can be hydrolyzed by CwlC. Tests show that CwlC is not only a key point of metrocyte lysis of B.thuringiensis in a spore period and has great significances in establishing B.thuringiensis engineering bacteria for efficient agricultural production, but also has great application prospects in biological medicines and food production.

The invention discloses a method for rapidly propagating potato minitubers by using potato minituber budlets. The method comprises specific steps as follows: germination acceleration of the potato minitubers: the potato minitubers are placed in a GA3 solution for soaking and then transferred into an illumination culture room at the constant temperature of 25 DEG C to send forth budlets; inductionrooting of budlets: the budlets are stripped from blastema of the potato minitubers and transferred into a nutrient solution until the budlets take root; substrate preparation: a substrate is preparedfrom vermiculite and perlite; transplantation of new seedlings: the new seedlings are cut in a seedbed, natural illumination is adopted, and water or a nutrient solution is sprayed according to nutrient conditions of plants after seedling recovery; the nutrient solution is sprayed according to the seedling condition 20 days after planting; the substrate is kept moist in the growing process of thenew seedlings; harvesting of minitubers: the minitubers can be harvested after the cutting seedlings grow for 35-40 days. The method has the advantages of short seedling culture time, high survival rate, high reproduction coefficient and low cost.

The invention discloses a method for inducing and regenerating plants at the base of rhizome buds of Hainan notoginseng, and belongs to the technical field of plant inducing regeneration. In this method, after the pre-treatment of the rhizome buds of Hainan notoginseng, the leaves and shoot tips on the rhizome buds are cut off, and the base of the buds is left as explants; the sterilized explants are inoculated on the induction medium and cultivated until the explants Clustered buds are formed at the base; when the clustered buds at the base of the primary buds grow to 4-6 cm, cut off the buds, intercept the 1.0-1.5 cm of the base of the buds and inoculate them into the proliferation medium to induce clustered buds; then cut off the clustered buds and transfer them to the rooting medium Carry out rooting, when the height of the seedlings on the rooting medium is 5-8 cm, harden the seedlings under natural light in the greenhouse for 7-10 days, take out the seedlings, wash the root medium, and obtain transplanted seedlings after cultivation. The invention significantly improves the tissue culture propagation efficiency of Hainan notoginseng.

A method for reduplicating the pollen chromosomes of eucommia tree by inducing features that a high-temp reduplicating device of tree is used to heat the male flower buds of eucommia tree in the meiophase of pollen metrocytes at 45-47 deg.C for 2 hr to increase the 2n pollen percentage to 45-70%, especially in the diakinasis-metaphase I.

The invention provides a quick selection method for strong-stem abelmoschus manilhot. The quick selection method for the strong-stem abelmoschus manilhot is characterized by comprising the following steps: A, abelmoschus manilhot seed selecting; B, abelmoschus manilhot seed sterilizing treatment; C, abelmoschus manilhot seed soaking and germination accelerating; D, rooting and plantlet hardening cultivation; E, abelmoschus manilhot blastema rooting and growing; F, abelmoschus manilhot blastema foundation bed cultivating; G, abelmoschus manilhot transplanting. The quick selection method for the strong-stem abelmoschus manilhot has the beneficial effects: by adopting the quick selection method, the planted abelmoschus manilhot has a thick stem, the amount of extracted gel is large, the root system of each plant is developed, and the yield of the abelmoschus manilhot is improved.

The invention discloses a method for quickly detecting the fruiting performance of pleurotus nebrodensis, which comprises the following steps of: inoculating pleurotus nebrodensis strains into the center of a solid culture medium, culturing for 7 to 10d at the temperature of 22 to 26 DEG C under a dark condition and culturing again for 3 to 5d after hyphae overgrow with a culture dish; then, freezing the culture dish at the temperature of 0 to 4 DEG C under a dark condition for 3 to 5d; and afterwards, putting the culture dish at the temperature of 8 to 12 DEG C under the illumination of 200 to 800Lux for carrying out recovering culture for 10 to 20d and selecting the strains which can form white blastemas with dense texture, i.e. the strains with better fruiting performance. The method has high identification and detection accuracy, short time and high efficiency, lowers the breeding or production cost and is suitable for the quick detection and identification of the fruiting performance of the pleurotus nebrodensis strains on a large scale.

The invention relates to the field of prevention and control of animal diseases, and in particular relates to application of hirsutella sinensis refined fermentation liquor as a bacteriostatic agent of bat moth infection paecilomyces farinosus, a paecilomyces farinosus bacteriostatic agent and a preparation method of the paecilomyces farinosus bacteriostatic agent. The bacteriostatic agent provided by the invention can be used for well inhibiting paecilomyces farinosus cultured in vitro and/or in vivo without causing any bad influence on bat moths. Although the bacteriostatic agent is applied continuously to infected moths, hirsutella sinensis in the moths can still live till the moths become stiff. Moreover, stiff moths can form a sporocarp blastema.

A process for culturing the polyploid abalone includes such steps as physical or chemical inducing to generate diploid/tetraploid abalone, culturing, crossing, choosing tetraploid abalone, and crossing between said tetrapliod abalone and diploid abalone to obtain triploid abalone.

The invention discloses a resurrection hormone composition capable of improving filling fat survival rate and reducing fat fibration and calcification. The resurrection hormone composition comprises, by weight, 20-26 parts of mother cells from receptor types, 20-24 parts of glucose base solution, 10-14 parts of recombinant human interleukin-II, 12-14 parts of cell factors, 1-2 parts of human PH (potential of hydrogen) regulators, 5-8 parts of amino acid, 6-9 parts of rh-a FGF (recombinant human-acidic fibroblast growth factors), 4-8 parts of cell growth stimulating activities GRO/MGSA and 0.5-1 part of lytic enzyme. Cells of types of same receptors serve as basic materials, cell organelles and the like in the basic materials are removed, cell sap is reserved, corresponding cell factors for assisting vital movement of the receptors are added, most of needed additives are from cell receptors, possibilities and seriousness of rejection reaction can be greatly reduced to some extent, special matching according to life characteristics of actual receptors can be omitted, production cost and technical requirements are effectively reduced, the application range of fat resurrection hormone is widened, and practicability of the fat resurrection hormone is improved.