Method for quickly detecting fruiting performance of pleurotus nebrodensis

Abstract

The invention discloses a method for quickly detecting the fruiting performance of pleurotus nebrodensis, which comprises the following steps of: inoculating pleurotus nebrodensis strains into the center of a solid culture medium, culturing for 7 to 10d at the temperature of 22 to 26 DEG C under a dark condition and culturing again for 3 to 5d after hyphae overgrow with a culture dish; then, freezing the culture dish at the temperature of 0 to 4 DEG C under a dark condition for 3 to 5d; and afterwards, putting the culture dish at the temperature of 8 to 12 DEG C under the illumination of 200 to 800Lux for carrying out recovering culture for 10 to 20d and selecting the strains which can form white blastemas with dense texture, i.e. the strains with better fruiting performance. The method has high identification and detection accuracy, short time and high efficiency, lowers the breeding or production cost and is suitable for the quick detection and identification of the fruiting performance of the pleurotus nebrodensis strains on a large scale.

Description

technical field

[0001] The invention belongs to a detection and identification method for edible fungi, and in particular relates to a method for rapidly detecting the fruiting performance of Pleurotus basilica. Background technique

[0002] Pleurotus nebrodensis, also known as Pleurotus nebrodensis, belongs to the Kingdom of Fungi, the Phylum Fungi, the Class Phytomycetes, the Order Agaricaceae, and the genus Pleurotus. It is a very precious large-scale edible fungus. Its fruiting body is white, the flesh of the bacteria is thick, crisp and delicious, and the fragrance is strong. It has the reputation of "G boletus". This kind of rare, beautiful and good-tasting fungus is known as "Xitianbai Ganoderma" among the people. It is relatively expensive and has high cultivation value.

[0003] Bailing Pleurotus mainly has two cultivation methods: agricultural cultivation and factory cultivation. These two cultivation methods usually take about 120 days from inoculation to fruitin...

Examples

Embodiment 1

[0027] Example 1 Screening and identification of fruiting performance of Pleurotus bailing strain

[0028] Proceed as follows:

[0029] There are 3 Pleurotus bailing strains involved in this test, numbered respectively 485, 489 and 1407, which are preserved in the China Agricultural Microorganism Culture Collection Center; wherein 485 strains are the wild bacterium collected from Xinjiang Mulei by the inventor in 2004. Bacterial strains; 489 bacterial strains are Zhongnong No. 1, which is the strain selected by the inventor through polyspore hybridization, and has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee in 2005. The preservation number is: CGMCC 1300 (see the patent "a kind of Bailing Mushroom New Variety and Strain Production and Cultivation Method" (patent number: ZL200510052226.7)); 1407 strain is a wild strain collected by the inventor in 2009 from Tacheng, Xinjiang.

[0030] (1) Culture medium pre...

Embodiment 2

[0034] Example 2 Bailing Pleurotus cultivation test

[0035] Proceed as follows:

[0036] (1) Cultivation raw material formula: 90% cottonseed hulls, 6% corn flour, 2% lime, 1% gypsum, 1% potassium dihydrogen phosphate, and the water content is 1:1.5-1.6.

[0037] (2) Inoculation time: inoculate on August 28, the strains are 485, 489 and 1407 strains in Example 1 respectively, and 100 bags of each variety are inoculated.

[0038] (3) Mycelia management: cultivating in a shading culture room, controlling the culture temperature at 20-26°C, and ventilating twice a day for 30 minutes each time. Turn the pile once every 10 days or so in order to produce bacteria evenly.

[0039] (4) Post-ripening conditions: after the hyphae are overgrown, the temperature of the cultivation room is controlled at 20-22° C., the relative air humidity is controlled at 70%, and a small amount of scattered light is appropriately given. After ripening time 40d.

[0040] (5) Budding-inducing methods ...

technical field

[0001] The invention belongs to a detection and identification method for edible fungi, and in particular relates to a method for rapidly detecting the fruiting performance of Pleurotus basilica. Background technique

[0002] Pleurotus nebrodensis, also known as Pleurotus nebrodensis, belongs to the Kingdom of Fungi, the Phylum Fungi, the Class Phytomycetes, the Order Agaricaceae, and the genus Pleurotus. It is a very precious large-scale edible fungus. Its fruiting body is white, the flesh of the bacteria is thick, crisp and delicious, and the fragrance is strong. It has the reputation of "G boletus". This kind of rare, beautiful and good-tasting fungus is known as "Xitianbai Ganoderma" among the people. It is relatively expensive and has high cultivation value.

[0003] Bailing Pleurotus mainly has two cultivation methods: agricultural cultivation and factory cultivation. These two cultivation methods usually take about 120 days from inoculation to fruitin...

Examples

Embodiment 1

[0027] Example 1 Screening and identification of fruiting performance of Pleurotus bailing strain

[0028] Proceed as follows:

[0029] There are 3 Pleurotus bailing strains involved in this test, numbered respectively 485, 489 and 1407, which are preserved in the China Agricultural Microorganism Culture Collection Center; wherein 485 strains are the wild bacterium collected from Xinjiang Mulei by the inventor in 2004. Bacterial strains; 489 bacterial strains are Zhongnong No. 1, which is the strain selected by the inventor through polyspore hybridization, and has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee in 2005. The preservation number is: CGMCC 1300 (see the patent "a kind of Bailing Mushroom New Variety and Strain Production and Cultivation Method" (patent number: ZL200510052226.7)); 1407 strain is a wild strain collected by the inventor in 2009 from Tacheng, Xinjiang.

[0030] (1) Culture medium pre...

Embodiment 2

[0034] Example 2 Bailing Pleurotus cultivation test

[0035] Proceed as follows:

[0036] (1) Cultivation raw material formula: 90% cottonseed hulls, 6% corn flour, 2% lime, 1% gypsum, 1% potassium dihydrogen phosphate, and the water content is 1:1.5-1.6.

[0037] (2) Inoculation time: inoculate on August 28, the strains are 485, 489 and 1407 strains in Example 1 respectively, and 100 bags of each variety are inoculated.

[0038] (3) Mycelia management: cultivating in a shading culture room, controlling the culture temperature at 20-26°C, and ventilating twice a day for 30 minutes each time. Turn the pile once every 10 days or so in order to produce bacteria evenly.

[0039] (4) Post-ripening conditions: after the hyphae are overgrown, the temperature of the cultivation room is controlled at 20-22° C., the relative air humidity is controlled at 70%, and a small amount of scattered light is appropriately given. After ripening time 40d.

[0040] (5) Budding-inducing methods ...

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