Method for quickly detecting fruiting performance of pleurotus nebrodensis

A technology of Pleurotus basilica and its performance, which is applied in the field of detection and identification of edible fungi, can solve the problems of no reports on rapid detection of the fruiting performance of the Pleurotus basilica strain, and achieve the effects of low cost, simple method and high efficiency

Inactive Publication Date: 2012-07-18
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] After searching, no report was found on the rapid detection of the fruiting performance of the Pleurotus basilica strain

Method used

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  • Method for quickly detecting fruiting performance of pleurotus nebrodensis
  • Method for quickly detecting fruiting performance of pleurotus nebrodensis
  • Method for quickly detecting fruiting performance of pleurotus nebrodensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Screening and identification of fruiting performance of Pleurotus ostreatus strain

[0028] Proceed as follows:

[0029] There are 3 Pleurotus ostreatus strains involved in this experiment, numbered respectively 485, 489 and 1407, which are preserved in the China Agricultural Microbial Culture Collection; among them, strain 485 is a wild strain collected by the inventor from Mulei, Xinjiang in 2004. Strain; Strain 489 is Zhongnong No. 1, which is selected by the inventor through polyspore hybridization. It has been deposited in the General Microbiology Center of China Microbial Culture Collection Management Committee in 2005. The preservation number is: CGMCC 1300 (see patent New Varieties of Bailing Mushrooms and Methods of Production and Cultivation of Strains" (Patent No.: ZL200510052226.7)); strain 1407 is a wild strain collected by the inventor from Tacheng, Xinjiang in 2009.

[0030] (1) Preparation of culture medium: add 200g / L potato (juice), 20g / L glucose, ...

Embodiment 2

[0034] Example 2 Pleurotus ostreatus cultivation test

[0035] Proceed as follows:

[0036] (1) Cultivation raw material formula: cotton seed hulls 90%, corn flour 6%, lime 2%, gypsum 1%, potassium dihydrogen phosphate 1%, and the water content is 1:1.5-1.6.

[0037] (2) Inoculation time: Inoculated on August 28, the strains were the 485, 489 and 1407 strains in Example 1, and 100 bags of each strain were inoculated.

[0038] (3) Hair mycelia management: culture in the culture room with shading, the culture temperature is controlled at 20~26℃, and ventilation is carried out twice a day for 30 minutes each time. In order to grow bacteria, turn the pile once every 10 days.

[0039] (4) Post-ripening conditions: After the mycelium is full, the temperature of the culture room is controlled at 20-22°C, the relative humidity of the air is controlled at 70%, and a small amount of scattered light is appropriately given. After ripening time 40d.

[0040] (5) Methods and conditions for invigorat...

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Abstract

The invention discloses a method for quickly detecting the fruiting performance of pleurotus nebrodensis, which comprises the following steps of: inoculating pleurotus nebrodensis strains into the center of a solid culture medium, culturing for 7 to 10d at the temperature of 22 to 26 DEG C under a dark condition and culturing again for 3 to 5d after hyphae overgrow with a culture dish; then, freezing the culture dish at the temperature of 0 to 4 DEG C under a dark condition for 3 to 5d; and afterwards, putting the culture dish at the temperature of 8 to 12 DEG C under the illumination of 200 to 800Lux for carrying out recovering culture for 10 to 20d and selecting the strains which can form white blastemas with dense texture, i.e. the strains with better fruiting performance. The method has high identification and detection accuracy, short time and high efficiency, lowers the breeding or production cost and is suitable for the quick detection and identification of the fruiting performance of the pleurotus nebrodensis strains on a large scale.

Description

Technical field [0001] The invention belongs to a detection and identification method for edible fungi, and specifically relates to a method for quickly detecting the fruiting performance of Pleurotus ostreatus. Background technique [0002] Pleurotus nebrodensis (Pleurotus nebrodensis), also known as Pleurotus nebrodensis, belongs to the genus of Fungi, Fungi, Layer Fungi, Agaricus and Pleurotus, and is a very precious large edible fungus. Its fruiting bodies are white, fleshy, crispy and delicious, and rich in fragrance. It has the reputation of "boletus". This kind of rare, beautiful, and good-tasting mushroom is known as "Xitian White Ganoderma lucidum" by the folks. The price is relatively high and the cultivation value is high. [0003] Pleurotus ostreatus mainly has two cultivation methods: agricultural cultivation and factory cultivation. These two cultivation methods usually take about 120 days from inoculation to fruiting. If the Pleurotus ostreatus strain with unclear ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04
Inventor 张金霞黄晨阳陈强高巍
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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