Method for efficiently inducing generation of adventitious roots of Pinus densiflora tissue culture plantlets

Abstract

The invention discloses a method for efficiently inducing the generation of adventitious roots of Pinus densiflora tissue culture plantlets, which uses growing young Pinus densiflora tissue culture plantlets as explants, and adopts a 'two-step method', namely firstly, inducing the generation of adventitious root blastemas in a culture medium containing 1 / 2 to 1 / 4 of WPM, 0.1 to 0.2 mg / L NAA, 1.0 to 2.0 mg / L IBA and 0.5 to 1.5 percent sucrose, and secondly, transferring the explants into a culture medium containing 1 / 2 of the WPM, 0.5 to 1.0 g / L AC and 2.0 percent of the sucrose to promote the expression and the extension of the adventitious roots so as to finish the generation process of the adventitious roots. By adopting the method, the generation ratio of the adventitious roots of the Pinus densiflora tissue culture plantlets is high, the root system develops well and the transplant survival rate is high, thus the method lays a solid foundation for the mass production of Pinus densiflora tissue culture regeneration plants for resisting pine wood nematode diseases, and simultaneously has important reference value for the cultivation of other pine species tissue culture plantlets.

Description

1. Technical field [0001] The invention belongs to the field of biotechnology, and specifically refers to a method for efficiently inducing adventitious roots of pine tissue culture seedlings to occur. 2. Background technology [0002] There are about 116 species, varieties and hybrids of Pinus, which are widely distributed and planted in the world, especially in temperate regions. Pinus not only has many types, but also often forms vast forests, so it is known as the "Northern Hemisphere". The Mother of the Forest". Pinus plants not only make outstanding contributions to the world's timber, turpentine and papermaking, but also play a very important role in the ecosystem. [0003] Red pine (Pinus densiflora) is a species of the genus Pinus, distributed in East China and the northern coastal areas of my country, as well as Japan, Korea, and the Soviet Union. With the characteristics of strong wind resistance, drought resistance and thin ridges, it is a pioneer tree species ...

AI Technical Summary

Problems solved by technology

Isikawa (1987) observed the formation of adventitious roots on the hypocotyls and seedling root tip organs in Chi pine; Choi et al. In +IBA0.5mg / L+NAA0.3mg / L rooting medium, a small amount of tissue cultured seedlings formed adventitious roots, and a large number of calluses were produced at the base of the tissue cultured seedlings, which seriously affected the quality of adventitious roots

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