Method for cultivating and producing sporocarps of dictyophora multicolor berk. and broome

A production method and a technique for bamboo sunflower seeds are applied in the field of cultivation and production of variegated bamboo fungus fruit bodies, and can solve problems such as limited resources and the like

Active Publication Date: 2011-09-07
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the resources of wild Dictyophora variegated fruiting bodies are extremely limited and are restricted by seas

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The raw material composition of comprehensive PDA slant medium is 200g of potatoes, 20g of glucose, 1.5g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, VB 1 10mg, 17g agar and 1000mL water, pH 6.0, the preparation method is to weigh 200g potatoes, wash, peel and chop, add 1000mL water and boil for 30min, filter with gauze, take the filtered juice and add glucose 20g, potassium dihydrogen phosphate 1.5g, sulfuric acid Magnesium 0.5, VB 1 10mg, 17g agar, fully dissolved, filtered with gauze while hot, take the filtered juice and pack into test tubes, each test tube is about 8mL depending on the size of the test tube, sterilize with 15 pounds of steam at 121°C for about 20 minutes, take out the test tube and put it on an inclined plane, cool and store for later use;

[0018] The fresh fruiting bodies of wild Dictyophora multicolor Berk. & Broome were separated from the tissue, and connected to the comprehensive PDA slant medium in the amount of about 0.5cm×0...

Embodiment 2

[0026] The fresh fruiting bodies of wild Dictyophora multicolor Berk. & Broome were separated from the tissue, and the size of about 0.5cm×0.5cm was connected to the comprehensive PDA slant medium, 10°C, humidity 50%, and cultured for 20d. The slant surface is covered with mycelia to obtain the slant mother species. The pH of the comprehensive PDA slant medium is 7.0, and its raw material composition is to add 150g of potatoes, 25g of glucose, 2g of potassium dihydrogen phosphate, 0.8g of magnesium sulfate, and VB in every 1000mL of water. 1 15mg, agar 20g, its preparation method is with embodiment 1.

[0027] Take 75kg of wheat (soaked in water for 30 hours, then rinsed with tap water, boiled in a pot for 38 minutes, filtered and dripped dry), 20kg of bamboo branch powder (soaked in hot water for 3 hours and then stacked and fermented for 5 days), 1kg of sucrose, 2kg of superphosphate, Mix 1.5kg of gypsum powder and 0.5kg of lime, and mix well. The pH is 7.0. At this time, th...

Embodiment 3

[0034] In addition to the comprehensive PDA slant medium, the raw materials are composed of 250g of potatoes, 15g of glucose, 1g of potassium dihydrogen phosphate, 0.3g of magnesium sulfate, and VB 1 5mg, agar 17g and 1000mL water, pH 5.0, and in the preparation method, the potatoes were washed, peeled and chopped, and boiled with water for 25min, other steps and conditions were the same as in Example 1.

[0035] The biological efficiency of the fruiting bodies of Dictyophora variegated this time reached 1.8kg / m 2 , that is, 1.8Kg of fresh Dictyophora variegated fruiting bodies can be obtained per square meter of production compost, and these variegated Dictyophora fruiting bodies can be used as raw materials for medicine or research materials.

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Abstract

The invention relates to a method for cultivating and producing sporocarps of Dictyophora multicolor Berk. and Broome (1882), which is characterized by comprising the following steps: carrying out the tissue isolation on the fresh sporocarps of the wild dictyophora multicolor berk. and broome (1882), inoculating the fresh sporocarps of the wild dictyophora multicolor berk. and broome (1882) to integrated PDA slope culture medium or pine needle decoction culture medium, culturing until a slope is covered by mycelia to obtain a slope maternal strain, inoculating the slope maternal strain to wheat grain culture medium, culturing until the wheat grain culture medium is covered by mycelia, selecting strains with white, thick and thick mycelia as original strains, sowing the original strains in culture medium for producing layer by layer, covering the culture medium for producing by soil, culturing until the mycelia grow out of the surface of the soil covered on the culture medium for producing, forming a large number of rhizomorphs from the mycelia, expanding the tail ends of the rhizomorphs to form young blastemas and buds, continuing culturing until indusiums have the largest opening degree, and harvesting the fresh sporocarps of the dictyophora multicolor berk. and broome (1882). Due to the adoption of the method, the biological efficiency of the sporocarps of the dictyophora multicolor berk. and broome (1882) can be 1.5-2.5kg/m<2>. The sporocarps of the dictyophora multicolor berk. and broome (1882) can be used as medicinal raw materials or research materials.

Description

technical field [0001] The invention relates to a production method of Dictyophora multicolor Berk. & Broome, in particular to a method for cultivating and producing fruiting bodies of Dictyophora multicolor Berk. & Broome. Background technique [0002] Dictyophora multicolor Berk. & Broome, also known as Dictyophora multicolor Berk. & Broome, belongs to Fungi, Basidiomycota, Agaricomycetes and Phallomycetidae in classification. ), Phallales, and Phallaceae are saprophytic fungi that are mostly scattered in bamboo forests and broad-leaved forests in summer. They are found in Jiangsu, Anhui, Fujian, Taiwan, Hunan, Guangdong, Guangxi, and Hainan , Guizhou, Yunnan and other tropical and subtropical provinces are distributed. Dictyophora variegated is slightly poisonous and should not be eaten, but it can be used for medicine. According to research, soaking its fruiting bodies in 70% alcohol for external application can treat athlete's foot, athlete's foot, enhance immunity, i...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 邹方伦宋斌吴兴亮李泰辉
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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