Method of cutturing polyploid seed breedign using diploid/tetraploid oclimera abalone

Abstract

A process for culturing the polyploid abalone includes such steps as physical or chemical inducing to generate diploid / tetraploid abalone, culturing, crossing, choosing tetraploid abalone, and crossing between said tetrapliod abalone and diploid abalone to obtain triploid abalone.

Description

technical field [0001] The invention relates to a breeding method for abalone polyploid, in particular to a method for polyploid breeding using diploid / tetraploid chimera abalone. Background technique [0002] The polyploid breeding of abalone has important biological significance and production significance. Shellfish triploid has the characteristics of growth advantage and good meat quality. The main purpose of breeding abalone polyploids is to produce sterile triploids for use in aquaculture and for transgenic research to prevent the spread of foreign genes into wild populations. [0003] At present, the methods for artificially directly inducing triploid shellfish usually include chemical methods (such as cytochalasin B, 6-DMAP) and physical methods (such as temperature shock, hydrostatic pressure, etc.) to inhibit the release of the first or second polar body. A large number of triploid seedlings are required in production, and it is difficult to completely rely on ar...

AI Technical Summary

Problems solved by technology

In the research on the artificial induction of abalone tetraploid by the above-mentioned physical or chemical methods, the number of tetraploid embryos or larvae obtained is very small, and most of them are deformed and dead, and cannot develop to metamorphosis, although a certain number of tetraploid embryos or larvae will be produced in the process. Somatic chimerism, but traditional induction of tetraploidy is considered a failure

Therefore, there has been no successful report so far in the study of inducing abalone tetraploid