Diabetes wounds have extremely complex hyperglycemia, hypoxia and high
reactive oxygen species (ROS) microenvironments. Consequently, modulation and management of this microenvironment may provide an updated and improved treatment approach for healing of diabetic wounds. In this case, we develop a multifunctional nano-
enzyme (CHA at GOx) for diabetes
wound treatment, which is jointly assembled based on special dual ligands (alendronate and dimethylimidazole) driven by Ce and
glucose oxidase (GOx). According to the present invention, the activity of GOx is protected, and the
simulation activity of
superoxide dismutase (SOD) and
catalase (CAT) is provided, such that the excess glucose and ROS can be effectively removed; particularly, excessive
hydrogen peroxide (H2O2) generated by glucose oxidation reaction can be catalyzed to generate
oxygen, so that the
oxygen balance of the
wound surface is adjusted, and the toxic and side effects of GOx are reduced. In addition, the
cerium nano-composite also has the effects of promoting
cell proliferation and migration and promoting
angiogenesis. In-vitro experiments show that CHA (at) GOx contributes to
cell migration and promotes formation of
cell tubes.
In vivo,
wound healing of diabetic mice can be obviously accelerated. In a word, the research shows that co-
assembly of the multifunctional nano-
enzyme has potential in
clinical treatment of diabetic wounds.