Cascade enzyme-like nano system for promoting healing of diabetic wounds and preparation method and application thereof

A technology for wound healing and diabetes, which is applied in the field of cascade enzyme-like nanosystems for promoting diabetic wound healing and its preparation, can solve the problems of inability to promote diabetic wound healing and unfavorable wound healing, and achieve excellent in vitro antibacterial effect, high efficiency and rapid Treatment of diabetic wounds, easily accessible results

Active Publication Date: 2021-06-15
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the inventors found that the presence of hydrogen peroxide in the wound part of the diabetic skin surface will make the skin at the wound site in a state of oxidative stress, which is not conducive to wound healing
Moreover, certain drug synergy must be added to promote wound healing, and it is impossible to promote diabetic wound healing without adding drugs

Method used

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  • Cascade enzyme-like nano system for promoting healing of diabetic wounds and preparation method and application thereof
  • Cascade enzyme-like nano system for promoting healing of diabetic wounds and preparation method and application thereof
  • Cascade enzyme-like nano system for promoting healing of diabetic wounds and preparation method and application thereof

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preparation example Construction

[0040] In one embodiment of the present disclosure, a preparation method of a cascade enzyme-like nanosystem for promoting diabetic wound healing is disclosed, comprising: mixing metal oxide nanoparticles with PAH solution, so that a PAH coating is formed on the surface of the metal oxide layer; then, glucose oxidase was added, and glucose oxidase was covalently conjugated to the amino group of PAH.

[0041] In some specific embodiments, the process of mixing the metal oxide nanoparticles with the PAH solution includes: configuring a NaCl solution containing PAH, the concentration of the PAH in the NaCl solution is 5-20 mg / mL, preferably, 10mg / mL.

[0042] In some specific embodiments, the metal oxide and the PAH solution are mixed and stirred at room temperature. After the reaction is completed, the mixed solution is centrifuged to obtain metal oxide / PAH nanoparticles; further, the stirring time is 2-5h , preferably, is 3h.

[0043] In some specific embodiments, before mixi...

Embodiment 1

[0053] A cascade enzyme-like nanosystem for promoting diabetic wound healing: Fe 3 o 4 -GOx nano system preparation method is as follows (principle such as figure 1 shown):

[0054] Material: Iron(III) Chloride Hexahydrate (FeCl 3 ·6H 2 O), sodium oleate, oleic acid, 1-octadecene, polyallylamine hydrochloride (PAH; Mw=15000Da), phosphate buffered saline (PBS), tetramethylammonium hydroxide 1-(3- Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide sodium salt (NHS), 3-(4,5-dimethyl-2 -thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and 2′,7′-dichlorodifluorofluorescein diacetate (DCFH-DA; ≥94%) were purchased from Sigma-Aldrich. 3,3',5,5'-tetramethylbenzidine (TMB), 2,2'-azidobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), o-Phenylenediamine (OPD; 98%), methylene blue (MB), terephthalic acid (TA) and hydrogen peroxide (H 2 o 2 ) analysis kit was purchased from Beyotime Biotechnology. Hydrogen peroxide (≥30%)...

Embodiment 2

[0060] A cascade enzyme-like nanosystem for promoting diabetic wound healing: MnO 2 -GOx nano system preparation method is as follows:

[0061] MnO 2 Preparation of nanoparticles: Put potassium permanganate into alkyl alcohol polyoxyethylene ether in batches at a molar ratio of 1:1 to 3, control the reaction temperature below 60°C, then stir for 3 to 4 hours, add anhydrous Ethanol, continue to stir for 30 minutes, filter out the solids, then reflux with absolute ethanol for 2 to 3 hours, and elute; filter again, filter out the solids as crude manganese dioxide, and then dry them at 75 to 85°C , crystallize the dried manganese dioxide at 400°C for 4 hours to obtain black nano manganese dioxide powder.

[0062] MnO 2 - Preparation of GOx nanosystem: Dissolve 0.1 g PAH in 10 mL of 1 mM NaCl solution, and add 1 mL of prepared MnO 2 nanoparticles. The mixture was stirred at room temperature at 900 rpm for 3 h to obtain MnO 2 / PAH solution. After the reaction is completed, th...

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Abstract

The invention relates to the technical field of biomedical materials, in particular to a cascade enzyme-like nano system for promoting healing of diabetic wounds and a preparation method and application thereof. The nano system comprises metal oxide nano particles and glucose oxidase modified on the surface of metal oxide. Glucose oxidase is modified on the surfaces of metal oxide nano-particles to obtain a cascade enzyme-like nano material which is used for regulating the micro-environment of the diabetic infected wounds and promoting the wounds to heal quickly.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a cascade enzyme-like nano system for promoting diabetic wound healing and its preparation method and application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] As we all know, diabetes seriously endangers people's health and poses a huge threat to people's lives. According to incomplete statistics, in 2015 there were approximately 415 million people suffering from diabetes worldwide, and this figure will rise to 642 million in 2040, which is worrying. And Chinese diabetes patients account for about 26.5% of the world, which is a very high rate. What's more se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/44A61K33/26A61K33/32A61K33/244A61K33/242A61K9/51A61K47/32A61K47/58A61K47/69A61P3/10A61P17/02A61P31/04C12N9/04
CPCA61K38/443A61K33/26A61K33/32A61K33/244A61K33/242A61K9/5138A61K47/58A61K47/6933A61P3/10A61P17/02A61P31/04C12N9/0006C12Y101/03004A61K2300/00
Inventor 李永强杜炫呈李伟峰贾秉清
Owner SHANDONG UNIV
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