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A cascade enzyme-like nanosystem for promoting diabetic wound healing and its preparation method and application

A wound healing and diabetes technology, which is applied in the field of cascade enzyme-like nanosystems for promoting diabetic wound healing and its preparation, can solve the problems of unfavorable wound healing, inability to promote diabetic wound healing, etc., and achieves excellent in vitro antibacterial effect, high efficiency and rapidity The effect of treating diabetic wounds and promoting wound healing

Active Publication Date: 2022-05-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the inventors found that the presence of hydrogen peroxide in the wound part of the diabetic skin surface will make the skin at the wound site in a state of oxidative stress, which is not conducive to wound healing
Moreover, certain drug synergy must be added to promote wound healing, and it is impossible to promote diabetic wound healing without adding drugs

Method used

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  • A cascade enzyme-like nanosystem for promoting diabetic wound healing and its preparation method and application
  • A cascade enzyme-like nanosystem for promoting diabetic wound healing and its preparation method and application
  • A cascade enzyme-like nanosystem for promoting diabetic wound healing and its preparation method and application

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preparation example Construction

[0040] In one embodiment of the present disclosure, a preparation method of a cascade enzyme-like nanosystem for promoting diabetic wound healing is disclosed, comprising: mixing metal oxide nanoparticles with PAH solution, so that a PAH coating is formed on the surface of the metal oxide layer; then, glucose oxidase was added, and glucose oxidase was covalently conjugated to the amino group of PAH.

[0041] In some specific embodiments, the process of mixing the metal oxide nanoparticles with the PAH solution includes: configuring a NaCl solution containing PAH, the concentration of the PAH in the NaCl solution is 5-20 mg / mL, preferably, 10mg / mL.

[0042] In some specific embodiments, the metal oxide and the PAH solution are mixed and stirred at room temperature. After the reaction is completed, the mixed solution is centrifuged to obtain metal oxide / PAH nanoparticles; further, the stirring time is 2-5h , preferably, is 3h.

[0043] In some specific embodiments, before mixi...

Embodiment 1

[0053] A cascade enzyme-like nanosystem for promoting diabetic wound healing: Fe 3 o 4 -GOx nano system preparation method is as follows (principle such as figure 1 shown):

[0054] Material: Iron(III) Chloride Hexahydrate (FeCl 3 ·6H 2 O), sodium oleate, oleic acid, 1-octadecene, polyallylamine hydrochloride (PAH; Mw=15000Da), phosphate buffered saline (PBS), tetramethylammonium hydroxide 1-(3- Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide sodium salt (NHS), 3-(4,5-dimethyl-2 -thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and 2′,7′-dichlorodifluorofluorescein diacetate (DCFH-DA; ≥94%) were purchased from Sigma-Aldrich. 3,3',5,5'-tetramethylbenzidine (TMB), 2,2'-azidobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), o-Phenylenediamine (OPD; 98%), methylene blue (MB), terephthalic acid (TA) and hydrogen peroxide (H 2 o 2 ) analysis kit was purchased from Beyotime Biotechnology. Hydrogen peroxide (≥30%)...

Embodiment 2

[0060] A cascade enzyme-like nanosystem for promoting diabetic wound healing: MnO 2 -GOx nano system preparation method is as follows:

[0061] MnO 2 Preparation of nanoparticles: Put potassium permanganate into alkyl alcohol polyoxyethylene ether in batches at a molar ratio of 1:1 to 3, control the reaction temperature below 60°C, then stir for 3 to 4 hours, add anhydrous Ethanol, continue to stir for 30 minutes, filter out the solids, then reflux with absolute ethanol for 2 to 3 hours, and elute; filter again, filter out the solids as crude manganese dioxide, and then dry them at 75 to 85°C , crystallize the dried manganese dioxide at 400°C for 4 hours to obtain black nano manganese dioxide powder.

[0062] MnO 2 - Preparation of GOx nanosystem: Dissolve 0.1 g PAH in 10 mL of 1 mM NaCl solution, and add 1 mL of prepared MnO 2 nanoparticles. The mixture was stirred at room temperature at 900 rpm for 3 h to obtain MnO 2 / PAH solution. After the reaction is completed, th...

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Abstract

The disclosure relates to the technical field of biomedical materials, specifically a cascade enzyme-like nanosystem for promoting diabetic wound healing and its preparation method and application. The nanosystem includes metal oxide nanoparticles and glucose modified on the surface of the metal oxide oxidase. A cascade-like nanomaterial was obtained by modifying glucose oxidase onto the surface of metal oxide nanoparticles, which was used to regulate the microenvironment of diabetic infected wounds and promote their rapid healing.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a cascade enzyme-like nano system for promoting diabetic wound healing and its preparation method and application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] As we all know, diabetes seriously endangers people's health and poses a huge threat to people's lives. According to incomplete statistics, in 2015 there were approximately 415 million people suffering from diabetes worldwide, and this figure will rise to 642 million in 2040, which is worrying. And Chinese diabetes patients account for about 26.5% of the world, which is a very high rate. What's more se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/44A61K33/26A61K33/32A61K33/244A61K33/242A61K9/51A61K47/32A61K47/58A61K47/69A61P3/10A61P17/02A61P31/04C12N9/04
CPCA61K38/443A61K33/26A61K33/32A61K33/244A61K33/242A61K9/5138A61K47/58A61K47/6933A61P3/10A61P17/02A61P31/04C12N9/0006C12Y101/03004A61K2300/00
Inventor 李永强杜炫呈李伟峰贾秉清
Owner SHANDONG UNIV
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