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Repairing agent for promoting wound healing of type 2 diabetes

A technology of accelerators and reagents, applied in the field of diabetes, to achieve the effect of promoting hair follicle growth, promoting hair follicle generation, and promoting wound healing

Active Publication Date: 2021-07-09
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current research proves that the chronic wounds of diabetic patients cannot be effectively controlled by the treatment method of single administration. Fast healing is important

Method used

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  • Repairing agent for promoting wound healing of type 2 diabetes
  • Repairing agent for promoting wound healing of type 2 diabetes
  • Repairing agent for promoting wound healing of type 2 diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Establishment of type 2 diabetes mouse model

[0036] (1) Purchase 100 C57 BL / 6 male mice from Beijing Weitong Lihua Experimental Animal Company, about 4 weeks old. After the mice arrive in the mouse room, feed them with ordinary feed for a week to make the mice gradually adapt to the mouse room Environment, let the mice live in an environment with a constant temperature of 25°C, moderate humidity, and alternating light and dark, without controlling the free drinking and eating of the mice;

[0037] (2) After 1 week, they were randomly divided into two groups, 50 rats in the type 2 diabetes group (T2D group), fed with high-sugar and high-fat feed; 50 rats in the normal control group (CHOW group), fed with ordinary basal feed;

[0038] (3) After the mice were fed in groups, the weight changes of the mice were recorded at a fixed time every week. When there was a significant difference in the weight of the two groups of mice, the blood glucose of the mice was me...

Embodiment 2

[0043] Isolation and culture of mouse adipose stem cells and fibroblasts

[0044] (1) Adipose stem cells: C57BL / 6 mice and T2D mice were killed by decapitation, the mice were sterilized with 75% alcohol, the abdomen of the mice was carefully cut open with sterilized scissors and forceps, and the mice were avoided. Hair, in order to avoid adipose tissue contamination, use tweezers to carefully pick up the white adipose tissue around the mouse epididymis, and put it into normal saline immediately;

[0045](2); Fibroblasts: kill the mice within one day after birth, soak them in 75% ethanol for 5 minutes, carefully cut the skin tissue on the back of the mice with sterilized scissors and tweezers, and put them in a double-antibody-containing in saline;

[0046] (3) Put the mouse adipose tissue and skin tissue into the ultra-clean workbench for operation, wash with 0.9% normal saline (containing double antibody) for 3 times, wash off the fat on the surface of the adipose tissue, an...

Embodiment 3

[0049] Example 3 Determination of the difference in expression of NPY in adipose stem cells derived from normal mice and adipose stem cells derived from type 2 diabetic mice

[0050] 1. RNA extraction from adipose-derived stem cells

[0051] (1) Cell extraction: Use a pipette to carefully absorb the culture medium, wash it twice with phosphate buffer, add 600 μl of lysate directly to the culture dish, and carefully pipette with a pipette to fully lyse the cells. Add the material to the DNA removal column; tissue extraction: Weigh approximately 20-30 mg of mouse tissue, put it into a 1.5 ml RNase free centrifuge tube, add 600 µl of lysate, grind it with an electric tissue grinder at high speed for about 1 min, and grind Try to avoid RNA degradation during the process. Put the ground tissue homogenate into a refrigerated centrifuge, centrifuge at 13,000 rpm for 3 min, carefully draw the tissue supernatant with a pipette gun and add it to the DNA removal column;

[0052] (2) Ce...

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Abstract

The invention provides a repairing agent for promoting wound healing of type 2 diabetes, and belongs to the technical field of diabetes. The repairing agent provided by the invention is composed of a low-concentration NPY solution and adipose-derived stem cells. By using the repairing agent provided by the invention, wounds of type 2 diabetes mice can be quickly and efficiently repaired. In addition, the repairing agent provided by the invention can effectively promote hair follicle generation and hair follicle growth at the wound, can effectively inhibit expression of pro-inflammatory genes IL-1 and TNF-alpha, and can promote expression of anti-inflammatory genes VEGF and TGF-beta.

Description

technical field [0001] The invention belongs to the technical field of diabetes, and in particular relates to a repairing agent for promoting type 2 diabetes wound healing. Background technique [0002] Type 2 diabetes is a common metabolic disorder, mainly due to insufficient insulin secretion or the rise of blood sugar level caused by insulin resistance, which leads to the occurrence of type 2 diabetes. Because the body is in a high blood sugar level for a long time, it is easy to cause a variety of complications, mainly including coronary heart disease, diabetic nephropathy, diabetic vascular disease and diabetic eye disease. At the same time, compared with healthy people, diabetic patients are more difficult to heal their wounds after injury, and the wound site is often accompanied by long-term inflammatory reactions, and chronic ulcers are prone to occur. [0003] Wound healing is a complex process that includes inflammation, proliferation, and tissue remodeling, and i...

Claims

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Application Information

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IPC IPC(8): A61K38/22A61K35/28A61P17/02A61P17/14
CPCA61K38/2271A61K35/28A61P17/02A61P17/14A61K2300/00
Inventor 董晓
Owner QINGDAO AGRI UNIV
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