The present invention provides a manufacturing process of red chenopodium quinoa polypeptide powder. The manufacturing process comprises the following steps: S1, high-temperature pretreatment of a redchenopodium quinoa substrate; S2, enzymatic hydrolysis of red chenopodium quinoa protease; S3, refined preparation of a chenopodium quinoa peptide solution; S4, concentration of the chenopodium quinoa peptide solution; and S5, sterilization, drying and granulation to obtain the red chenopodium quinoa polypeptide powder. The process is simple and easy for large-scale industrial production. The obtained red chenopodium quinoa polypeptide powder has characteristics of easy digestion, easy absorption, resistance, etc., also has unique biological activities of preventing oxidation, lowering bloodpressure and lowering blood glucose, is suitable for women preparing for pregnancy, people with acidic physique, people with malnutrition and low immunity, and patients with high blood pressure, highblood lipid, and high blood sugar, opens a new way for conversion of high added value of red chenopodium quinoa, and also provides theoretical basis for deep layer development and utilization of multi-functional nutritional food.