Application of geranyl flavone A in preparation of medicines for promoting wound healing
A technology of geranyl flavonoids and wound healing, applied in the field of medicine, can solve problems such as susceptibility to trauma, difficulty in recovery, weakened body defense ability of diabetics, etc.
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Embodiment 1
[0134] Embodiment 1: Construction of type II diabetes model
[0135] Method for constructing type II diabetes model by using low-dose streptozotocin and high-fat diet in combination [7] It is a relatively mature method at present, and has the advantages of short time-consuming, high modeling success rate, good repeatability, and low mortality rate.
[0136] 1. Experimental animals and grouping
[0137] 25 male rats (C57BL / 6, purchased from Victoria Lihua) aged 6-8 weeks, with an average body weight of more than 20 g.
[0138] Control group (control): 5 rats were intraperitoneally injected with 0.1 mmol / L citrate buffer solution at pH 4.2.
[0139] Experimental group (STZ): 50 rats, intraperitoneally injected with 1% streptozotocin; 25 rats were randomly selected for statistics.
[0140] 2. Model making method
[0141] Before modeling, the initial blood glucose and body weight of the mice were detected on an empty stomach, as follows: Figure 1A , Figure 1C shown.
[0...
Embodiment 2
[0147] Embodiment 2: skin wound healing experiment
[0148] 1. Experimental method
[0149] (1) Construction of skin wound model
[0150] C57 wild adult male mice (C57BL / 6, purchased from Victoria Lihua Company) were taken, the back hair was depilated after anesthesia, and a full-thickness wound was constructed with a 6mm biopsy punch. Pay attention to disinfection and prevent infection.
[0151] (2) Grouping and administration method
[0152] Normal control group: 5 rats, no administration.
[0153] Vaseline group: Vaseline (medical Vaseline, purchased from Tianjin Zhiyuan Chemical Reagent Co., Ltd., the same below), 5 mg Vaseline per mouse, 5 mice.
[0154] Drug A group: 10 mg / rat, 10 rats (flavone control, drug A is soybean isoflavone).
[0155] B drug group: 10 mg / rat, 10 rats (flavonoid control, B drug is quercetin).
[0156] Cannflavin A group: Cannflavin A, 10 mg / rat, 10 rats.
[0157] The administration methods of the latter 4 groups were all applied to the woun...
Embodiment 3
[0170] Embodiment 3: the skin wound healing experiment of staphylococcus aureus infection (1)
[0171] The wounds of diabetic patients are not only difficult to heal but also prone to infection. Staphylococcus aureus is the most common pathogen in human suppurative infection, which can cause local suppurative infection, pneumonia, pseudomembranous enteritis, pericarditis, etc., and even sepsis and sepsis Wait for systemic infection. In this study, Staphylococcus aureus was used to construct a skin infection model of diabetic mice [8] , which aims to be closer to the diseases of human wounds that are not easy to heal, making the research more meaningful.
[0172] 1. Experimental method
[0173] (1) Model making method
[0174] Take normal mice as controls and type Ⅱ diabetic mice constructed according to the method in Example 1, and after anesthetizing and hair removal on the back, construct full-thickness wounds with a 6 mm biopsy punch. Inoculate 1.5x10 6 CFU / ml Staphy...
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