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Chronic wound healing composition and application thereof

A technology for chronic wounds and pharmaceutical compositions, applied in the direction of drug combinations, active ingredients of hydroxyl compounds, active ingredients of heavy metal compounds, etc.

Inactive Publication Date: 2021-06-08
罗胥恩
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports of its use in the treatment of chronic wound healing

Method used

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  • Chronic wound healing composition and application thereof
  • Chronic wound healing composition and application thereof
  • Chronic wound healing composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0055] Drugs: (1) Acteoside (Acteoside, code-named A): purchased from Sigma-Aldrich (St.Louis, MO, USA).

[0056] (2) Bismuth subgallate (Bismuth subgallate, code-named BSG): American Sigma-Aldrich (St.Louis, MO, USA).

[0057] (3) Borneal (code-named BO): American Sigma-Aldrich (St.Louis, MO, USA).

[0058] According to the present invention, compositions can be prepared according to the following composition:

[0059]

[0060]

[0061] Carry out the content of each component of the composition of the present invention of following cell experiment:

[0062] (1) Acteoside 16.0 μM (0.01 μg / μl), that is, 0.1%;

[0063] (2) Bismuth subgallate 14.2 μM (0.0056 μg / μl), ie 0.056%; and

[0064] (3) Borneo BO 18.8 μM (0.0029 μg / μl), ie 0.029%.

[0065] Cell culture:

[0066] Human dermal fibroblast (HDF cells) or skin keratinocytes (human keratinocytes, HaCaT cells) were cultured in a humid incubator at 37°C in an atmosphere of 5% carbon dioxide and 95% oxygen. Place in Dul...

example 1

[0067] Example 1 Cell Growth Analysis (Cell Proliferation Assay)

[0068] Take HaCaT cells 3 x 10 5 Add 25mM glucose to the standard and culture in a 12-well petri dish for 48 hours. Cell growth and viability were analyzed with the CCK-8 reagent kit (Dojindo Molecular Technologies, Kumamoto, Japan), 5 μL of CCK-8 solution was added to each cell and incubated at 37°C for one hour, and then the spectrophotometer Microplate reader (SpectraMax, Molecular Device, USA) was used to measure the absorption at 450nm, and the sample was compared with the value of the control group to analyze the experimental results. The experimental groups tested included:

[0069] (1) Con: control group;

[0070] (2) A 16.0 μM;

[0071] (3) BO 18.8μM;

[0072] (4) BSG 14.2μM;

[0073] (5) BO 18.8μM+A 16.0μM;

[0074] (6) BSG 14.2μM+A 16.0μM;

[0075] (7) BO 18.8μM+BSG 14.2μM;

[0076] (8) All (A 16.0 μM+BO 18.8 μM+BSG 14.2 μM).

[0077] The results of cell proliferation after 48 hours of cult...

example 2

[0078] Example 2 low sugar and high sugar environment cell culture analysis comparison (Comparison in Cell Proliferation Assay)

[0079] Take HaCaT cells 3 x 10 5 Cultured with 5mM and 25mM glucose, cultured in a 12-well petri dish for 48 hours, added the composition of the present invention (All:A 16.0μM+BO 18.8μM+BSG 14.2μM) to observe the cell proliferation status.

[0080] The results of cell proliferation after 48 hours of culture are as follows: figure 2 , showing that the composition of the present invention (A 16.0μM+BO 18.8μM+BSG 14.2μM) has a significant synergistic effect on cell growth in high-sugar culture (25mM), but does not significantly promote cell proliferation in low-sugar (5mM) effect.

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Abstract

The invention relates to a chronic wound healing composition and application thereof, in particular to a composition with a chronic wound healing effect, especially a composition with a diabetic wound healing effect. The composition mainly comprises the following three components: (1) an anti-inflammatory agent selected from a group consisting of acteoside, isoacteoside and a combination of the acteoside and the isoacteoside; (2) an astringent selected from a group consisting of gallic acid, subgallic acid, salts thereof and a combination of the gallic acid, the subgallic acid and the salts; and (3) a freshener selected from a group consisting of borneol, menthol and a combination of the borneol and the menthol, and optionally combined with one or more pharmaceutically acceptable carriers as needed. The invention also provides application of the composition to preparation of a medicament for treating chronic wounds, especially diabetic wounds.

Description

technical field [0001] The present invention relates to compositions with chronic wound healing effects and their use. Background technique [0002] With the aging population, chronic diseases have become the main axis of health care, and the care of chronic wounds is a very important part. Chronic wounds refer to wounds that cannot heal in general wound structure and appearance after proper treatment for a predetermined period of time, and there is still no effective therapeutic agent so far. Types of chronic wounds include decubitus or pressure sores caused by long-term bed rest, wound ulcers in diabetic patients, limb gangrene with peripheral vascular obstruction, ulcers caused by arterial or venous disorders, non-healing wounds in cancer patients, and chronic wounds caused by acute wound infections. Poor healing and more. [0003] Acteoside, an active ingredient in Chinese herbal medicine, has lipase inhibitory effects and is known to have antibacterial, anti-inflammat...

Claims

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Application Information

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IPC IPC(8): A61K31/7032A61K31/045A61K31/555A61P17/02A61P3/10
CPCA61K31/7032A61K31/045A61K31/555A61P17/02A61P3/10A61K2300/00A61K31/29A61K31/7036A61K31/192A61K45/06A61K31/7034
Inventor 罗胥恩
Owner 罗胥恩
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