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Novel cerium-mediated co-assembled multifunctional nano-enzyme (CHA at GOx) for diabetes wound treatment

A multifunctional, nano-enzyme technology, applied in the fields of nano-enzyme materials and regenerative medicine, can solve the problems of poor drug-carrying capacity, inhibiting catalytic efficiency, etc., and achieve the effects of easy commercialization, improved drug delivery, and good biological safety.

Pending Publication Date: 2022-05-13
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the drug loading capacity of cerium nanoparticles is poor, while traditional metal-organic frameworks with good drug delivery capacity related to cerium are rarely reported, because their micron size greatly inhibits the catalytic efficiency.

Method used

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  • Novel cerium-mediated co-assembled multifunctional nano-enzyme (CHA at GOx) for diabetes wound treatment
  • Novel cerium-mediated co-assembled multifunctional nano-enzyme (CHA at GOx) for diabetes wound treatment
  • Novel cerium-mediated co-assembled multifunctional nano-enzyme (CHA at GOx) for diabetes wound treatment

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0047] The optimization of example 1 organic double ligand

[0048] Add 1 mL each of alendronate sodium (AL: 1, 5, 10, 15, 20 mg / mL) and dimethylimidazole (HMIM: 1, 5, 10, 15, 20 mg / mL) by the controlled variable method, and magnetically After stirring and mixing, 100 μL of cerium nitrate solution [Zn(NO3)2.6H2O] (0.1M) was added slowly, and stirred at room temperature for 2 hours. Finally, after 24 hours of dialysis with ultrapure water, the product was collected and measured by DLS. The particle size of the reaction product, with the smallest particle size as the standard, determines the final reaction concentration as AL (10 mg / mL), HMIM (10 mg / mL).

example 2

[0049] Nanoparticle synthesis of example 2 cerium ion mediation

[0050] Take 1 mL each of AL (10 mg / mL) and HMIM (10 mg / mL), stir and mix with a magnetic force, then slowly add 100 μL of cerium nitrate solution [Zn(NO3)2.6H2O] (0.1M). Similarly, after reacting for 2 hours, it was purified by dialysis, and the particle size of nanoparticles was observed by TEM, SEM and AFM.

example 3

[0051] Preparation of Example 3CHA@GOx

[0052] Take 1 mL each of AL (10 mg / mL) and HMIM (10 mg / mL), stir and mix with a magnetic force, slowly add 100 μL of cerium nitrate solution [Zn(NO3)2.6H2O] (0.1M), and then add 100 μL of glucose oxidase solution (1 mg / mL), and stirred at room temperature for 2 hours, and finally dialyzed with ultrapure water for 24 hours to obtain pure CHA@GOx.

[0053] The multifunctional nanozyme formed based on cerium-mediated glucose oxidase co-assembly was characterized as follows:

[0054] figure 1 Schematic diagram of the synthesis of CHA@GOx.

[0055] figure 2 It is the TEM, SEM and AFM images of the nanoparticles mediated by cerium ions. By comparison, it can be seen that the nanoparticles mediated by cerium ions are uniformly dispersed, and the particle size is about 8nm.

[0056] image 3 The TEM images, AFM images, and UV and IR absorption images of CHA@GOx. From the results of transmission electron microscopy and atomic force micros...

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Abstract

Diabetes wounds have extremely complex hyperglycemia, hypoxia and high reactive oxygen species (ROS) microenvironments. Consequently, modulation and management of this microenvironment may provide an updated and improved treatment approach for healing of diabetic wounds. In this case, we develop a multifunctional nano-enzyme (CHA at GOx) for diabetes wound treatment, which is jointly assembled based on special dual ligands (alendronate and dimethylimidazole) driven by Ce and glucose oxidase (GOx). According to the present invention, the activity of GOx is protected, and the simulation activity of superoxide dismutase (SOD) and catalase (CAT) is provided, such that the excess glucose and ROS can be effectively removed; particularly, excessive hydrogen peroxide (H2O2) generated by glucose oxidation reaction can be catalyzed to generate oxygen, so that the oxygen balance of the wound surface is adjusted, and the toxic and side effects of GOx are reduced. In addition, the cerium nano-composite also has the effects of promoting cell proliferation and migration and promoting angiogenesis. In-vitro experiments show that CHA (at) GOx contributes to cell migration and promotes formation of cell tubes. In vivo, wound healing of diabetic mice can be obviously accelerated. In a word, the research shows that co-assembly of the multifunctional nano-enzyme has potential in clinical treatment of diabetic wounds.

Description

technical field [0001] The invention relates to a preparation method of a multifunctional nanozyme formed by cerium-mediated co-assembly with an enzyme, in particular to a preparation of a multifunctional nanozyme material formed by co-assembling a double ligand and glucose oxidase mediated by cerium The method, which is further used in the treatment of diabetic wounds, belongs to the field of nano-enzyme materials and regenerative medicine. Background technique [0002] Diabetes has become one of the major diseases threatening human health, causing a large number of deaths every year, and the most common diabetic syndrome is diabetic ulcer. It is a chronic complication characterized by extremely slow wound healing. The main reason for the difficult healing of diabetic wounds is its special microenvironment, such as high glucose, hypoxia and high reactive oxygen species (ROS) levels. It is worth noting that these disadvantages are not independent of each other. Typically,...

Claims

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Application Information

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IPC IPC(8): A61K38/44A61K33/244A61K47/24A61K47/22A61P17/02A61P3/10G01N27/327
CPCA61K38/443A61K33/244A61K47/24A61K47/22A61P17/02A61P3/10C12Y101/03004G01N27/3272A61K2300/00
Inventor 于超余晓娟付晓雪
Owner CHONGQING MEDICAL UNIVERSITY
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