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Preparation method of nano-composite hydrogel and application of nano-composite hydrogel in promoting healing of diabetic wounds

A nano-composite, wound-healing technology, applied in pharmaceutical formulations, prostheses, bandages, etc., can solve the problems of no breakthrough in chronic wound treatment, low delivery efficiency, low immunogenicity, etc., and achieve broad market application prospects and good consumption Action, selective effect

Pending Publication Date: 2022-01-21
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have problems such as drug side effects, low delivery efficiency, need for anesthesia, and immune rejection. No breakthrough has been seen, therefore, a new strategy with high efficiency and low immunogenicity for diabetic wound healing is urgently needed

Method used

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  • Preparation method of nano-composite hydrogel and application of nano-composite hydrogel in promoting healing of diabetic wounds
  • Preparation method of nano-composite hydrogel and application of nano-composite hydrogel in promoting healing of diabetic wounds
  • Preparation method of nano-composite hydrogel and application of nano-composite hydrogel in promoting healing of diabetic wounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] A kind of preparation method of nanocomposite hydrogel is as follows (material modification method such as figure 1 shown):

[0056](1) Add soluble silk fibroin (molecular weight: 2,000 to 10,000 Daltons) powder in deionized water to make a 10g / L silk fibroin aqueous solution. Under the conditions, 0.5 equivalent of glycidyl methacrylate (GMA) was added and stirred to dissolve, and the reaction was continued for 6 hours. After the reaction, the reaction solution was transferred to a dialysis bag and dialyzed in deionized water for 3 days. The purified solution obtained after dialysis was lyophilized to obtain glycidyl methacrylate modified silk fibroin polymer (SF-GMA), and the lyophilized sample was dissolved and characterized by hydrogen nuclear magnetic resonance spectrometer to obtain the sample. 1 H-NMR spectrum as figure 2 shown.

[0057] (2) Add carboxymethyl cellulose (molecular weight: 90,000 Daltons) powder into deionized water to make a 10g / L carboxymeth...

Embodiment 2

[0062] (1) Add soluble silk fibroin (molecular weight: 2,000 to 10,000 Daltons) powder in deionized water to make a 10g / L silk fibroin aqueous solution. After it is completely dissolved, adjust the pH to 4.7. Under the conditions, 1.0 equivalent of glycidyl methacrylate (GMA) was added and stirred to dissolve, and the reaction was continued for 6 hours. After the reaction, the reaction solution was transferred to a dialysis bag and dialyzed in deionized water for 3 days. The purified solution obtained after dialysis was freeze-dried to obtain glycidyl methacrylate-modified silk fibroin polymer (SF-GMA).

[0063] (2) Add carboxymethyl chitosan (carboxylation degree 80%) powder to deionized water to make 20g / L carboxymethyl chitosan aqueous solution, after completely dissolving, adjust the pH to 5.0, Add 1 equivalent of glycidyl methacrylate (GMA) under the condition of ℃ and stir to dissolve, and continue to react for 8h. After the reaction, the reaction solution was transfer...

Embodiment 3

[0067] (1) Add gelatin (molecular weight: 50,000 to 100,000 Dalton) particles in deionized water, dissolve at 40°C to make a 50g / L gelatin aqueous solution, and after completely dissolving, add 1 equivalent of formazan at a reaction temperature of 50°C Acrylic anhydride (MA) was stirred and dissolved, and the reaction was continued for 2h. After the reaction, the reaction solution was transferred to a dialysis bag and dialyzed in deionized water for 3 days. The purified solution obtained after dialysis was freeze-dried to obtain methacrylic anhydride-modified gelatin polymer (GelMA).

[0068] (2) Add carboxymethyl cellulose (molecular weight: 90,000 Dalton) powder into deionized water to make 20g / L carboxymethyl cellulose aqueous solution. After it is completely dissolved, adjust the pH to 5.0, Under the conditions, 1.5 equivalents of glycidyl methacrylate (GMA) was added and stirred to dissolve, and the reaction was continued for 6 hours. After the reaction, the reaction so...

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Abstract

The invention provides a preparation method of nano-composite hydrogel and application of the nano-composite hydrogel in promoting healing of diabetic wounds. The method comprises the following steps: preparing a functionally modified natural high-molecular polymer and functional metal oxide nanoparticles, mixing the functionally modified natural high-molecular polymer solution with the functional metal oxide nanoparticles, and finally preparing the nano composite hydrogel by means of enzymatic crosslinking and photo-crosslinking. The hydrogel prepared by the method has multiple functions, and can be used for adjusting the pathological microenvironment of the diabetic wound to promote the healing of the diabetic wound.

Description

technical field [0001] The invention belongs to the field of tissue engineering and polymer materials, and in particular relates to a preparation method of a nanocomposite hydrogel and its application in promoting diabetic wound healing. Background technique [0002] The information in the background technology section of the present invention disclosure is only intended to increase the understanding of the general background of the present invention, and is not necessarily regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art known to those skilled in the art. [0003] Chronic diseases, including diabetes, cardiovascular and cerebrovascular diseases, hypoxia, cancer and immunosuppression, etc. will cause the formation of chronic wounds, and chronic wounds cannot be repaired normally, orderly and timely, which has caused great harm to both patients and the medical system. burden. Among them, diabetic wounds will undergo exces...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F299/00C08F2/48C08J3/075C08L55/00A61L26/00A61L27/02A61L27/20A61L27/22A61L27/50A61L27/52A61L27/56A61L27/60
CPCC08F299/00C08F2/48C08J3/075A61L27/56A61L27/52A61L27/60A61L27/50A61L27/025A61L27/227A61L27/20A61L27/222A61L26/0038A61L26/008A61L26/0085A61L26/0061A61L26/0004A61L26/0047A61L26/0023C08J2355/00A61L2400/12A61L2400/06C08L89/00C08L1/286C08L5/08
Inventor 迟波蒲雅婕王鹏辉
Owner NANJING UNIV OF TECH
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