A method of inducing jujube 2n pollen based on branch instillation technology

Abstract

The invention discloses a method for simply and efficiently inducing jujube 2n pollen based on a stem dripping technology. The method comprises the following steps of: drilling bases of stems of a jujube tree by using colchicines as a mutagenic agent at an exuberant meiosis stage of jujube pollen mother cells; and directly dripping the colchicines to the stems, so that medicine liquor is upwards transported by the xylems, and spindle fibers are prevented from being generated in the reduction mitosis process of the pollen mother cells so as to induce the pollen chromosome to be doubled to obtain the 2n pollen. The method provided by the invention is applied to introducing the 2n pollen; the introducing time is easily grasped, and the 2n pollen introducing can be carried out on a plurality of jujube trees within one year; and the high-proportion 2n pollen is obtained to quicken the sexual polyploidization breeding process of the jujube trees.

Description

technical field [0001] The invention relates to a method for inducing 2n pollen of jujube tree based on branch instillation technology. Background technique [0002] Jujube (Ziziphus jujuba Mill.) is the largest dry fruit tree species in my country and has developed rapidly in recent years. In order to adapt to the rapid development of jujube industry, the breeding of new varieties is imminent. Because jujube flowers are small, difficult to emasculate, and embryos are highly aborted, jujube hybrid breeding is difficult, and ploidy breeding has become one of the important ways to cultivate new jujube varieties. [0003] The polyploid obtained through asexual polyploidy cannot realize the recombination of excellent genes because there is no introgression of foreign excellent genes, so it is urgent to carry out ploidy breeding of jujube trees through sexual polyploidy. [0004] 2n pollen (also known as giant pollen) is of great value in sexual ploidy breeding. According to t...

AI Technical Summary

Problems solved by technology

The inventors of this patent have tried to use smearing method and minimally invasive method to induce giant pollen, and the smearing method is time-consuming and laborious, and the efficiency is very low; the ratio of 2n pollen induced by the minimally invasive method can reach 18.3%, but because the minimally invasive method is relatively It is also labor-intensive to cut a thin single jujube hanger, and the induction efficiency still cannot meet the needs of large-scale breeding practice.

Images