The invention provides a taurine substituted bodipy fluorescent compound, and further provides a preparation method of the taurine substituted bodipy fluorescent compound. The method comprises the following steps: reacting 8-(4-position substituted phenyl)-3, 5-diiodo-1, 2, 6, 7-tetra-substituted bodipy and 1,2-disubstituted taurine under the conditions of an additive, a catalyst and a solvent toobtain 8-(4-position substituted phenyl) 3-iodo-5-(2-ethanesulfonic acid) amine)-1,2,6,7-tetra-substituted bodipy and 8-(4-position substituted phenyl) 3,5-bis((2-ethanesulfonic acid) amine)-1,2,6,7-tetra-substituted bodipy. According to the taurine substituted bodipy fluorescent compound, by a cheap metal catalyzed direct C-X/N-H activating method, taurine with biological activity is introducedat a 3,5 activated site of star fluorescent molecule bodipy, and therefore, a novel fluorescent molecule framework is formed.