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Biological detection chip, biosensor and preparation method thereof and application

A biosensor, biodetection technology, applied in the measurement of color/spectral properties, etc., can solve the problem of unsuitable immobilization of membrane proteins and other membrane surface molecules, and achieve the effects of enriching species, enhancing infrared absorption, and reducing signal-to-noise ratio.

Pending Publication Date: 2020-04-24
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved in the present invention is to overcome the defect that the SEIRA biosensor in the prior art can only be used for the detection of fixed types of molecules to be detected, and is not suitable for fixing membrane surface molecules such as membrane proteins.

Method used

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  • Biological detection chip, biosensor and preparation method thereof and application
  • Biological detection chip, biosensor and preparation method thereof and application
  • Biological detection chip, biosensor and preparation method thereof and application

Examples

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Embodiment 1

[0053] This implementation provides a biological detection chip 11, such as figure 1 As shown, it includes a stacked substrate 111 , a metal array layer 112 and a film adsorption layer 113 , wherein the metal array layer 112 is located on the substrate 111 and has a spacer region 1122 exposing the substrate 111 . The film absorbing layer covers the metal array layer 112 and the surface of the substrate 111 located in the spacer region 1122 , and has a hydrophilic surface.

[0054] In existing SEIRA-based biosensors, in order to detect target molecules, it is usually necessary to immobilize specific recognition molecules that bind to target molecules on the metal surface of the SEIRA biosensor. However, since the metal surface can only immobilize a limited number of specific recognition molecules, the types of target molecules that can be detected by SEIRA biosensors are limited, and it is not suitable for the analysis of complex and diverse biomolecules on the membrane surface...

Embodiment 2

[0067]This embodiment provides a biological detection chip 11, which differs from the biological detection chip 11 in Embodiment 1 in that:

[0068] like image 3 As shown, a biofilm component 114, specifically a vesicle, is also included. As a kind of organelle with biomembrane structure, vesicle is suitable for loading various membrane surface molecules on the phospholipid bilayer of biomembrane. At the same time, the vesicles can be adsorbed on the membrane adsorption layer 113 with the hydrophilic phosphate head of the phospholipid bilayer, so that the membrane surface molecules are attached to the membrane adsorption layer 113 of the biological detection chip 11, and the surface of the membrane surface molecules is obtained Enhanced infrared spectral information can realize the detection and real-time dynamic analysis of molecules on the surface of a single membrane.

[0069] Vesicles, as a kind of membranous organelles widely present inside and outside cells, are easy ...

Embodiment 3

[0071] This embodiment provides a biosensor, such as Figure 4 As shown, the biosensor includes the biodetection chip 11 and the housing 12 in the first embodiment. Wherein, the housing 12 has an inner chamber 123 , and an inlet 121 and an outlet 122 connecting the inner chamber 123 with the outside world. Specifically, such as Figure 4 As shown, the inlet 121 and the outlet 122 are respectively opened on two opposite vertical side walls of the casing 12 . The solution to be tested containing target biomolecules enters the inner chamber 123 of the housing 12 through the inflow port 121 , circulates in the inner chamber 123 , and then flows out of the housing 12 through the outflow port 122 .

[0072] An installation hole is provided on the side wall of the housing 12 avoiding the inlet 121 and the outlet 122. Specifically, the installation hole is located on the top wall of the housing 12 and is a through hole penetrating the top wall of the housing 12 for biological detect...

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Abstract

The invention discloses a biological detection chip. The biological detection chip comprises a substrate, a metal array layer and a film adsorption layer which are stacked, wherein the metal array layer is positioned on the substrate and is provided with a spacing region for exposing the substrate; the film adsorption layer wraps the metal array layer and the surface of the substrate located in the spacing region, and the film adsorption layer is provided with a hydrophilic surface. Cells and organelles with biological film structures can be adsorbed by utilizing the hydrophilic surface of thefilm adsorption layer, target biomolecules are loaded on a biological film, the surface enhanced infrared absorption spectrum of the target biomolecules can be obtained, and detection and analysis ofthe target molecules are realized. The invention discloses a biosensor, which comprises the biological detection chip, is suitable for detecting film surface biomolecules with complex types, does notdestroy the molecular structure, and is suitable for research of various physiological and pathological processes, drug screening diagnosis and the like. The invention discloses a preparation methodof a biological detection chip, which is simple in preparation process and suitable for preparing the chip for detecting the biomolecules on the surfaces of various films.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a biological detection chip, a biosensor, a preparation method and an application thereof. Background technique [0002] Infrared spectroscopy is the absorption spectrum for studying molecular motion. As a clear, non-destructive and label-free substance detection method, infrared spectroscopy has become an indispensable analytical method. Using infrared spectroscopy to study the vibration of biological macromolecules can obtain information on the structural changes of biomolecules under physiological conditions, and infrared light has no damage to biological samples, making infrared spectroscopy a useful tool for studying the structural changes of biomolecules and revealing the nature of life phenomena. one of the important means. However, due to the low molecular absorption cross-sectional area of ​​infrared vibrations, which limits the intensity of molecular infra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35
CPCG01N21/35
Inventor 刘丽炜王诗琪屈军乐任升
Owner SHENZHEN UNIV
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