Method for preparing composite biological fertilizer by combined solid fermentation of double bacterium

A composite biological bacteria and solid fermentation technology, applied in the direction of organic fertilizers, etc., can solve the problems of long fermentation period of bacteria, unstable product performance, and difficult to guarantee quality, so as to reduce production costs, reduce the probability of bacterial contamination, The effect of shortening the fermentation cycle

Inactive Publication Date: 2009-11-25
ANHUI JINNONG HUIMIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, the production of microbial fertilizers currently used is only to purchase strains for fermentation and mix them with solid attachments by physical stirring. Not only does the fermentation cycle of strains be long and the production cost is high, but also the product performance is not good due to random compounding. Stable, so that the spore formation rate of the product is low, and the quality is difficult to guarantee

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1 Preparation of secondary jelly-like bacillus seeds

[0010] 1. Cultivate jelly-like bacillus (bacteria purchased from the strain preservation center of Beijing Institute of Microbiology, Chinese Academy of Sciences) first-class seeds by shaking the shaker flask, the shaker temperature is 26-30 ° C, the shaker speed is 180-200 rpm, The pH value is 7.7-7.9, and the growth cycle is 14-15 hours.

[0011] In the primary medium formula, the weight percentage of each component is as follows: 84% starch, 0.85% ammonium sulfate, 15% NaCL, and 0.15% sucrose.

[0012] 2. Place the secondary seed culture medium in the seed tank and perform sterilization treatment. The sterilization temperature of the seed tank liquid is 121 ° C, the holding time is 30 minutes, and the pH value of the seed liquid after sterilization is 7.7-7.9; then the primary The seeds are inoculated into the seed tank, so that the temperature in the seed tank is controlled at 28-30 ° C, and the tank pr...

Embodiment 2

[0015] Example 2 Preparation of secondary Bacillus megaterium seeds

[0016] 1. Cultivate the first-class seeds of Bacillus megaterium (the bacteria are purchased from the strain preservation center of Beijing Institute of Microbiology, Chinese Academy of Sciences) by shaking the shaker flask, the temperature of the shaker is 30°C, the speed of the shaker is 180-200 rpm, and the pH value is 7.5- 7.8, the growth cycle is 14 hours.

[0017] 2. Place the secondary seed medium in the seed tank and carry out sterilization treatment. The sterilization temperature of the seed tank liquid is 121 ° C, the holding time is 30 minutes, and the pH value of the seed liquid after sterilization is 7.5-7.8; then the primary Seeds are inoculated into the seed tank, and the inoculation amount is 10% of the total amount (first-level seed + second-level seed medium), so that the temperature in the seed tank is controlled at 30 ° C ± 1, and the tank pressure is 0.4-0.6 kg / cm 2 , the speed of the ...

Embodiment 3

[0021] Production of compound biological fertilizer by combined solid-state fermentation of Bacillus jelly-like and Bacillus megaterium

[0022] (1) Weigh the components of the solid medium according to the formula requirements, totaling 100 kg, including 23 kg of bran, 30 kg of straw, 15 kg of fly ash, 6 kg of bean cake powder, CaCO 3 (quicklime) 0.7 kg, grass carbon 10 kg, K 2 HPO 4 0.3 kg, 3 kg of sucrose, 12 kg of loam, the straw is crushed to 20 mesh, and the rest of the components are in powder form;

[0023] All the above components are purchased from the market, K 2 HPO 4 It is a powder product.

[0024] (2) mix each component and stir evenly, send into solid fermenting tank and carry out sterilization, after solid fermenting tank is sealed, heat up to 121 ℃ and keep warm for 2 hours, cool down naturally for use;

[0025] (3) Second-level jelly-like bacillus seeds and second-level bacillus megaterium seeds in the seed tanks of embodiment 1 and embodiment 2 were r...

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PUM

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Abstract

The invention relates to a method for preparing a composite biological fertilizer from tremellose bacillus and gigantochromoblast, which comprises: culturing and growing secondary tremellose bacillus strain and secondary gigantochromoblast strain; then, (1) stirring the solid culture medium uniformly, and placing into a disinfected solid fermentation pool, wherein, the weight percentage of the components are: wheat bran 15-25%, straw 25-36%, fly ash 11-19%, bean cake powder 3-9%, CaCO3 0.3-0.9%, turf 7.5-14%, K2HPO4 0.1-0.6%, sucrose 1-5%, and fertilizer soil 9-15%; (2) inoculating the two secondary strains into the solid fermentation pool according to that: the inoculation amount is 20% of the total weight, while the amount of the secondary tremellose bacillus strain is twice of the secondary gigantochromoblast strain; (3) adding water into the solid fermentation pool to keep the humidity of the ferment material at 50%, sealing and controlling the temperature in the solid fermentation pool, and fermenting and growing for 48-54 hours to obtain the finished product. The invention adopts combined solid fermentation of double bacterium, accelerates the strain breeding speed, shortens the fermentation period, reduces production cost, and improves the blastema formation ratio of the product.

Description

technical field [0001] The invention relates to a microbial fertilizer production technology, in particular to a method for preparing compound biological fertilizer by using bacillus jelly-like bacillus and bacillus megaterium. Background technique [0002] In the prior art, microbial fertilizers are usually fermented with a single bacterial species in a separate liquid, and then stirred with solid attachments to produce a single-performance microbial fertilizer. In recent years, there have also been reports on the development of composite bacterial agents, but the fermentation production process used is basically that each strain is fermented in liquid alone, and then mixed with solid attachments. For example, people such as Tang Xinyun reported some problems in the production and application of compound microbial fertilizers in the article "Microbiological Fertilizer and Its Production and Application" ("Journal of Biology", Volume 18, No. 1, February, 2000). Cui Zhengzho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08
Inventor 田广斌张六六李国
Owner ANHUI JINNONG HUIMIN BIOTECH CO LTD
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