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Method for inducing doubling of pollen chromosome of Eucommia ulmoides

A technology of chromosome doubling and eucommia pollen, which is applied in the field of doubling the number of chromosomes in plant pollen, can solve the problems of high toxicity, inability to obtain 2n pollen, ineffective chromosome doubling of eucommia pollen, etc., and achieve a high ratio effect

Inactive Publication Date: 2009-02-04
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] However, 2n pollen cannot be obtained by heating Eucommia male flower buds at the temperature of 32-42 °C disclosed in the above-mentioned prior art, indicating that this temperature range is ineffective in inducing chromosome doubling of Eucommia pollen
[0004] In addition, in the article "Research on Doubling Chromosomes of Eucommia Eucommia Induced by Colchicine" published in "Journal of Beijing Forestry University" No. 4, 2004, Gao Peng et al. disclosed a method of inducing 2n pollen with colchicine solution, The eucommia ulmoides 2n pollen ratio obtained by this method is relatively low, and the average 2n pollen percentage of the best treatment is only 27.62%; in addition, the poisonous effect of chemicals such as colchicine is large, resulting in flower buds not loose powder or very low amount of loose powder, which is difficult to apply Eucommia triploid breeding practice

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Films were made to observe the meiotic process of pollen mother cells of Eucommia ulmoides. During the pachytene stage of pollen mother cell meiosis, male flower buds of Eucommia ulmoides were continuously heated at 47°C for 2 hours with a tree high temperature doubler.

[0012] The pollen was collected after the pollen matured, and the 2n pollen percentage was counted. The results are shown in Table 1.

[0013] The equipment for heating Eucommia male flower buds in the method of the present invention can adopt the tree high-temperature doubler disclosed in the patent application for invention with publication number CN1669408A (application number: 200510011598.5) filed by the applicant on April 20, 2005.

Embodiment 2

[0015] Films were made to observe the meiosis process of pollen mother cells of Eucommia ulmoides. During the pachytene stage of meiosis of pollen mother cells, male flower buds of Eucommia ulmoides were continuously heated at 47°C for 4 hours with a tree high temperature doubler.

Embodiment 3

[0017] Films were made to observe the meiosis process of pollen mother cells of Eucommia ulmoides. During the determinative stage to metaphase I of pollen mother cell meiosis, the male flower buds of Eucommia ulmoides were continuously heated at 47°C for 2 hours with a tree high temperature doubler.

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Abstract

A method for reduplicating the pollen chromosomes of eucommia tree by inducing features that a high-temp reduplicating device of tree is used to heat the male flower buds of eucommia tree in the meiophase of pollen metrocytes at 45-47 deg.C for 2 hr to increase the 2n pollen percentage to 45-70%, especially in the diakinasis-metaphase I.

Description

technical field [0001] The invention relates to a method for improving genotype, in particular to a method for doubling the number of plant pollen chromosomes. Background technique [0002] At present, the methods for inducing pollen chromosome doubling mainly include chemical methods (such as colchicine induction method) and physical methods (such as abnormal temperature induction method). Among them, the method of using abnormal temperature induction is usually to artificially induce chromosome doubling by creating a high temperature environment higher than the abnormal temperature state in the growth and development period of the plant. Generally speaking, one or two-year-old herbaceous plants, small shrubs that can be planted in pots, and arbors that can be matured by cutting branches and hydroponic nutrient seeds, etc., can be subjected to high temperature treatment in an incubator to induce the doubling of male gamete chromosomes, and obtain diploid pollen (referred to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08
Inventor 康向阳高鹏陈洪伟张正海
Owner BEIJING FORESTRY UNIVERSITY
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