Method for inducing doubling of pollen chromosome of Eucommia ulmoides
A technology of chromosome doubling and eucommia pollen, which is applied in the field of doubling the number of chromosomes in plant pollen, can solve the problems of high toxicity, inability to obtain 2n pollen, ineffective chromosome doubling of eucommia pollen, etc., and achieve a high ratio effect
Examples
Embodiment 1
[0011] Films were made to observe the meiotic process of pollen mother cells of Eucommia ulmoides. During the pachytene stage of pollen mother cell meiosis, male flower buds of Eucommia ulmoides were continuously heated at 47°C for 2 hours with a tree high temperature doubler.
[0012] The pollen was collected after the pollen matured, and the 2n pollen percentage was counted. The results are shown in Table 1.
[0013] The equipment for heating Eucommia male flower buds in the method of the present invention can adopt the tree high-temperature doubler disclosed in the patent application for invention with publication number CN1669408A (application number: 200510011598.5) filed by the applicant on April 20, 2005.
Embodiment 2
[0015] Films were made to observe the meiosis process of pollen mother cells of Eucommia ulmoides. During the pachytene stage of meiosis of pollen mother cells, male flower buds of Eucommia ulmoides were continuously heated at 47°C for 4 hours with a tree high temperature doubler.
Embodiment 3
[0017] Films were made to observe the meiosis process of pollen mother cells of Eucommia ulmoides. During the determinative stage to metaphase I of pollen mother cell meiosis, the male flower buds of Eucommia ulmoides were continuously heated at 47°C for 2 hours with a tree high temperature doubler.
PUM
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Description
Claims
Application Information
- IPC
- A01H1/08
- Inventors
- 康向阳; 高鹏