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Method for high-density fermentation of bacillus subtilis strain and application

A Bacillus subtilis, high-density fermentation technology, applied in microorganism-based methods, applications, chemical instruments and methods, etc., can solve problems such as high production costs and complex production processes, and achieve improved efficiency, short fermentation time, and improved immunity. Effect of globulin and antibody levels

Inactive Publication Date: 2020-06-30
山东天润和生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The production process disclosed in CN108277179A is complicated, and a feeding tank is required for feeding, and an acid-base tank is required for pH regulation; the formula disclosed in CN102433283A contains high-priced raw materials such as yeast powder and peptone, and the production cost is relatively high

Method used

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  • Method for high-density fermentation of bacillus subtilis strain and application
  • Method for high-density fermentation of bacillus subtilis strain and application
  • Method for high-density fermentation of bacillus subtilis strain and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Activate the strain of Bacillus subtilis

[0047] Streak the strain of Bacillus subtilis preserved in the glycerol tube on the plate, culture it upside down in the incubator at 37°C for 24 hours, and cultivate a single colony;

[0048] The plate medium is: 0.3% beef extract, 0.5% peptone, 0.5% sodium chloride, 1% glucose, 0.5% agar powder, the balance is water, make up 100%, pH7.0; Take the larger single colonies of vigorous bacterial colonies, spread them on the slope, and cultivate them for 2 days, until more than 90% of the thallines produce spores.

[0049] The slant medium is: 0.5% of yeast extract powder, 1% of tryptone, 1% of sodium chloride, 1% of glucose, and the balance is water, supplemented by 100%, pH7.0.

[0050] (2) Preparation of Bacillus subtilis seed liquid

[0051] Use the inoculation loop to pick the bacterial lawn on the inclined surface of the test tube and inoculate it into the seed shaker bottle, the inoculum size is: 1cm 2 / 200ml, culture...

Embodiment 2

[0056] Fermentation method is the same as embodiment 1, the consumption of soybean meal powder in the fermentation medium in (3) is controlled at 36g / L, the consumption of cornstarch is controlled at 36g / L, and all the other raw materials are unchanged.

[0057] After the fermentation, the analysis results showed that the number of live bacteria in the fermentation broth was 4.05×10 10 cfu / ml.

Embodiment 3

[0059] The fermentation method is the same as in Example 1, the soybean meal consumption is controlled at about 40g / L in the fermentation medium in (3), the cornstarch consumption is controlled at 45g / L, and all the other raw materials are unchanged.

[0060] After the fermentation, the analysis results showed that the number of live bacteria in the fermentation broth was 4.19×10 10 cfu / ml.

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Abstract

The invention belongs to the technical field of microorganism strain fermentation, in particular relates to a method for high-density fermentation of a bacillus subtilis strain, and further relates toapplication of the strain to feed additives and agricultural planting. The method for high-density fermentation of the bacillus subtilis strain comprises the following steps: (1) activating a strainof bacillus subtilis; (2) preparing a bacillus subtilis seed liquid; and (3) performing tank fermentation. The method has the beneficial effects that (1) the high-density liquid fermentation method ofthe bacillus subtilis has a final fermentation level of 40 billion / ml or greater; and (2) the method is short in fermentation time, that is, about 18-24 hours, the production cost can be reduced, theefficiency can be improved, the fermentation level is high, and the spore formation rate is high.

Description

technical field [0001] The invention belongs to the technical field of fermentation of microbial strains, in particular to a method for high-density fermentation of bacillus subtilis strains, and also relates to the application of the above strains. Background technique [0002] Bacillus subtilis is widely distributed in soil and decayed organic matter. There have been some research reports on the high-density cultivation of Bacillus subtilis probiotics. For example, the following patent documents disclose the high-density fermentation method of Bacillus subtilis: [0003] CN108277179A discloses a high-density fermentation and spray-drying method of Bacillus subtilis, which is characterized in that it comprises the following steps: (a), strain activation: Streak the Bacillus subtilis preserved on a slant at a temperature of 4°C in NA culture On the base, culture at 30-37°C for 20-30h; (b), preparation of seed liquid: pick activated colony, inoculate into sterilized broth med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/30A01N63/22A01P3/00A01P1/00A01P21/00C09K17/14C12R1/125C09K101/00
CPCA01N63/00A23K10/18A23K50/30C09K17/14C09K2101/00C12N1/20
Inventor 郭芳坤刘鹏曹洪亮高健丁仁涛曹新春
Owner 山东天润和生物工程有限公司
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