Coccidian oocyst culture preservative fluid and application method thereof

Abstract

The invention belongs to the field of veterinary parasites, and relates to coccidian oocyst culture preservative fluid and an application method thereof. The invention is characterized in that the culture preservative fluid takes polyhaxemethylenguanidine hydrochloride as a main ingredient. The application method of the coccidian oocyst culture preservative fluid comprises the following steps of: separating and purifying fresh coccidian oocysts from fowl dung or intestinal tract tissues containing the coccidian oocysts; adding an appropriate amount of a polyhaxemethylenguanidine hydrochloride solution; adjusting the density of the oocysts; controlling the depth of a culture medium; putting the culture medium in a shaking table to perform shaking culture; performing microscopic examination; stopping culture when the sporulation rate of the oocysts exceeds 85%; collecting the oocysts; adding an appropriate amount of the polyhaxemethylenguanidine hydrochloride solution again; and preserving in a refrigerator of which the temperature is 4 DEG C. The coccidian oocyst culture preservative fluid is efficient, long-acting and safe, pollution-free to the environment, simple to prepare and low in cost, and is ideal coccidian oocyst culture preservative fluid. By applying the application method provided by the invention, the operation is simple and convenient, the efficiency is high, the automation can be realized, and the coccidian oocyst culture preservative fluid is suitable for large-scale culture preservation of coccidian oocysts.

Description

Technical field [0001] The invention belongs to the field of veterinary parasites, and relates to a coccidian oocyst culture preservation solution and an application method. Background technique [0002] Coccidiosis is an economically important intestinal parasitic disease caused by Eimeria coccidia in the intestines of chickens, which can reduce weight gain and feed returns. The disease is widely distributed and is often endemic, posing a great threat to the chicken industry. For a long time, effective drugs have been used to prevent and treat chicken coccidiosis, but the long-term use of these drugs inevitably leads to the production of drug-resistant strains, and their residues in meat and eggs also endanger human health. The preventive measures based on chicken coccidiosis vaccine immunity are the ideal method to control coccidiosis. Compared with anti-coccidiosis drugs, the application of chicken coccidiosis vaccine has three advantages: First, there is no drug resistance ...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the safety and potential environmental pollution of the existing coccidian oocyst culture preservation solution potassium dichromate solution and the unstable properties of the chloramine T solution, and obtain a safe, efficient and stable spheroid Coccidia oocyst culture preservation solution, and provide a method for culturing coccidia oocysts that is easy to operate and suitable for large-scale application

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