Method for promoting formation of bacillus coagulans spores and microecological preparation and application thereof

A technology of Bacillus coagulans and microecological preparations, applied in the method and its microecological preparations, to promote the field of Bacillus coagulans spore formation, which can solve problems such as unfavorable spore formation

Active Publication Date: 2020-10-30
FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In fact, in actual production, high-density bacterial liquid can be obtained by increasing the concentration of fermentation medium. However, nutrient-rich medium is often not conducive to the formation of spores, and only in the case of nutrient deficiency is more conducive to the formation of spores.

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  • Method for promoting formation of bacillus coagulans spores and microecological preparation and application thereof

Examples

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preparation example Construction

[0058] Preferably, the preparation method of the bacillus coagulans spore liquid comprises the following steps:

[0059] Pick 2-3 rings of Bacillus coagulans strains from the slant, inoculate them in a 1000mL triangular flask with 100mL of seed solution, rotate at 200r / min, and incubate at 35-40°C for 60-68h until the microscopic examination of the spore formation rate Higher than 90%, the Bacillus coagulans spore liquid is prepared.

[0060] In a preferred embodiment of the present invention, the method comprises the following steps:

[0061] (A), preparation of seed solution: inoculate the Bacillus coagulans strain into the seed solution medium for seed solution cultivation, and then inactivate the bacteria that have not formed spores to obtain the Bacillus coagulans seed solution;

[0062] (B), thalline expansion: inoculate the Bacillus coagulans seed solution obtained in step (A) in the expansion culture medium, carry out expansion culture, and obtain the expansion cultur...

Embodiment 1

[0122] A method for promoting the formation of bacillus coagulans spores, said method comprising the following steps:

[0123] (A), preparation of seed solution: Inoculate the Bacillus coagulans strain in 100mL seed solution medium at a mass ratio of 1%, and culture it in a shaker flask at a speed of 200r / min for 68h at 35°C, and perform staining and microscopic examination The spore formation rate of Bacillus coagulans is greater than 90%, and then treated in a water bath at 85°C for 30 minutes to inactivate the bacteria that have not formed spores to obtain the Bacillus coagulans seed solution;

[0124] In the seed liquid culture medium, each 1L seed liquid culture medium includes the following components: peptone 8g, yeast extract powder 5g, glucose 2g, sodium chloride 5g and dipotassium hydrogen phosphate 2g;

[0125] (B), thalline expansion: inoculate the Bacillus coagulans seed solution obtained in step (A) in the expansion culture medium with a mass ratio of 0.5‰, carry...

Embodiment 2

[0135] A method for promoting the formation of bacillus coagulans spores, said method comprising the following steps:

[0136] (A), preparation of seed solution: except that the composition of seed solution culture medium is different, all the other are with embodiment 1;

[0137] In the seed liquid culture medium, each 1L seed liquid culture medium includes the following components: peptone 9g, yeast extract powder 4g, glucose 3g, sodium chloride 8g and dipotassium hydrogen phosphate 2.5g;

[0138] (B), thalline expansion culture: except that the composition of expansion culture medium is different, all the other are with embodiment 1;

[0139] In the expansion culture medium, each 1L expansion culture medium contains the following components: peptone 4g, yeast extract powder 3g, soybean meal powder 25g, corn flour 18g, bran 23g, anhydrous glucose 13g, enteromorpha powder 6g, Dipotassium hydrogen phosphate 2.5g, magnesium sulfate heptahydrate 0.04g, manganese sulfate monohyd...

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Abstract

The invention provides a method for promoting formation of bacillus coagulans spores and a microecological preparation and application thereof, and relates to the technical field of micro-ecological preparations. The method comprises that an induction liquid is added in the fermentation culture process, and the induction liquid is bacillus coagulans spore liquid. The problem that the concentrationof viable bacteria and the sporulation rate are difficult to consider at the same time often exists, according to the method, bacillus coagulans spore liquid is creatively added in the fermentation culture process to serve as induction liquid for fermentation culture, so that the spore production rate of bacillus coagulans is effectively increased, the spore forming time is shortened, the production efficiency is greatly improved, and the production cost is reduced.

Description

technical field [0001] The invention relates to the technical field of probiotics, in particular to a method for promoting the formation of bacillus coagulans spores, as well as probiotics and applications thereof. Background technique [0002] With the advent of the era of complete ban on antibiotics in feed, antibiotics are no longer allowed in feed. Therefore, the breeding industry is facing unprecedented challenges. As one of antibiotic replacement products, probiotics can regulate intestinal health, improve immunity, improve feed digestion and utilization, and reduce waste discharge. Commonly used bacteria in microecological preparations include Bacillus subtilis, Bacillus licheniformis, Enterococcus faecalis, Enterococcus faecium, acidophilus lactic acid bacteria, Lactobacillus plantarum, Saccharomyces cerevisiae, etc. Among them, lactic acid bacteria have poor stress resistance and short shelf life. has not been widely used. [0003] In recent years, a new class of ...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12N1/38C12N1/20A23K10/18C12R1/07
CPCC12N3/00C12N1/38C12N1/20A23K10/18
Inventor 赖水明张招荣陈毅诚张书金吴贤峰吴有林
Owner FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD
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