Method for promoting aspergillus flavus to produce spores rapidly

A flavus, rapid technology, applied in the field of life sciences, can solve the problems of long mycelium culture time, low mycelium sporulation efficiency, and affect the progress of scientific research, so as to improve the sporulation rate, shorten the cultivation period, and save manpower and material resources Effect

Active Publication Date: 2018-11-20
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the laboratory, the Aspergillus flavus spore suspension is generally prepared after plate-cultured mycelium and natural spore production, but the mycelium culture takes a long time, usually 7 days. Due to the long culture time, it is easy to cause pollution, and mycelium production The low efficiency of spores seriously affects the progress of related scientific research

Method used

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  • Method for promoting aspergillus flavus to produce spores rapidly
  • Method for promoting aspergillus flavus to produce spores rapidly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043](1) Take out the Aspergillus flavus stored in a plastic freezing tube at -70°C, immediately place it in a 38°C-40°C water bath for rapid recovery and shake appropriately. until all the ice inside is dissolved. The strains were inoculated into PDA medium and cultured for 1 d.

[0044] (2) The activated Aspergillus flavus was re-transferred to the plate of PDA solid medium, and placed in a constant temperature incubator at 28°C for 72 hours.

[0045] (3) When the colony grows to a diameter of 3cm, use an inoculation loop to take a piece of about 0.8cm in size 2 Mycelia at the edge of the colony were transplanted into PDA solid medium.

[0046] (4) Reseal the plate with the mycelia connected and continue to cultivate for 48h in a constant temperature incubator at 28°C.

[0047] (5) A small amount of yellow-green spores were seen in the plate after culturing for 48 hours, and the spores were washed with Tween water to prepare a suspension of Aspergillus flavus spores. Di...

Embodiment 2

[0050] Preparation of graphene PDA medium:

[0051] a 2g large sheet (500nm-5μm lateral size) graphene was dissolved in 200mL ultrapure water, ultrasonicated to homogeneity, then centrifuged at 4000rpm for 30 minutes, and the supernatant was taken. After high temperature sterilization at 121°C for 30min, a large sheet was made Graphene aqueous solution.

[0052] b. Measure 200g potato, 20g glucose, and 20g agar, dilute to 500mL, and make PDA solid medium solution after high temperature sterilization at 121℃ for 30min. heated to dissolve).

[0053] c. Mix the large sheet graphene aqueous solution with the uncooled PDA solid medium solution in a volume ratio of 1:1, pour it into a flat plate for cooling, and obtain a graphene PDA medium for inducing sporulation.

[0054] (1) Take out the Aspergillus flavus stored in a plastic freezing tube at -70°C, immediately place it in a 38°C-40°C water bath for rapid recovery and shake appropriately. until all the ice inside is dissolved...

Embodiment 3

[0060] Preparation of small sheet graphene PDA solid medium:

[0061] a Dissolve 0.2 g of small sheet graphene (50-200 nm) in 200 mL of sterile water, and after ultrasonication to homogeneity, the small sheet graphene aqueous solution was aseptically operated in a biological safety cabinet, and passed through a 0.22 μm microporous membrane to make small pieces Layer graphene aqueous solution is ready for use.

[0062] b Weigh 200g of potato, 20g of glucose, and 20g of agar, dilute to 500mL, and make a PDA medium solution after high temperature sterilization at 121℃ for 30min (Note: Do not let the medium solution drop to room temperature, if it drops to room temperature, use a microwave oven heated to dissolve).

[0063] c Mix the small sheet graphene aqueous solution with the uncooled PDA solid medium at a volume ratio of 1:1, pour it into a flat plate for cooling, and obtain a graphene PDA medium for inducing sporulation.

[0064] (1) Take out the Aspergillus flavus stored ...

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Abstract

The invention discloses a method for promoting aspergillus flavus to produce spores rapidly. The method comprises the following steps: taking a graphene doped solid culture medium as a culture mediumand inducing mycelium of the aspergillus flavus to produce the spores rapidly. The method has the following advantages: the spore-producing experimental period of the mycelium of the aspergillus flavus is shortened; and the mycelium of the aspergillus flavus can be induced rapidly to produce a large amount of spores within 48 to 72 hours. Good basis is laid for later research of the aspergillus flavus; a large amount of precious time is saved for scientific research workers; and high application potential is achieved.

Description

technical field [0001] The invention belongs to the technical field of life science, and particularly relates to a rapid sporulation method of Aspergillus flavus. Background technique [0002] Toxin pollution caused by Aspergillus fungus (especially Aspergillus flavus) is collectively referred to as Aspergillus toxin pollution, with a wide range of hosts, resulting in aflatoxin widely existing in rice, corn, peanuts, sesame, soybean, rapeseed and other grain and oil foods, a serious threat Human life and health. At present, the spore suspension of Aspergillus flavus is generally prepared by plate culture mycelium and natural spore production in the laboratory, but the culture time of the mycelium is long, usually 7 days. The spore efficiency is low, which seriously affects the progress of related scientific research. The research on Aspergillus flavus spores is very common, and it is extremely important for the research on the production mechanism of aflatoxin, the anti-in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12N1/14C12R1/67
CPCC12N1/14C12N3/00
Inventor 喻理李培武杨代斌张奇朱婷婷董雪丽姜俊
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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