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Locust microsporidia AL200801 strain as well as biological preparation and application thereof

An AL200801 and locust microsporidia technology, which is applied to the locust microsporidia AL200801 strain and its biological preparations and application fields, can solve the problems of high production cost, low strain lethality, low sporulation rate and the like

Active Publication Date: 2021-11-16
北京嘉景生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low lethality, low spore production rate and high production cost of the selected Microsporidia locust strains in China, they have not been widely used in China.

Method used

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  • Locust microsporidia AL200801 strain as well as biological preparation and application thereof
  • Locust microsporidia AL200801 strain as well as biological preparation and application thereof
  • Locust microsporidia AL200801 strain as well as biological preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Screening method for Microsporidia locust AL200801 strain:

[0033] 1. Go to the place where the locusts occurred to collect the sick locusts. The diseased locusts are selected as follows: locusts whose abdomen is significantly larger than that of ordinary locusts and whose movements are slower.

[0034] 2. Grinding, filtering, microscopically examining, centrifuging and purifying the diseased insects, and separating different wild locust microsporidia strains from the insect bodies.

[0035] 3. Apply each microsporidian strain of wild locust to the leaves of corn or wheat at a concentration of 0.4 billion spores / ml, feed the 3rd instar locust gnats of migratory locust with the obtained leaves for 3 days, and only harvest and feed the dead ones after 3 days Insect body.

[0036] 4. Continue to separate the insect bodies obtained in step 3 from the dead insect bodies according to the method described in step 2 to obtain different strains of No. locust locusts.

[0037...

Embodiment 2

[0039] sporulation rate test

[0040] The sporulation method of this sporulation rate test adopts live hosts for propagation, and the live hosts are East Asian migratory locusts, and the newly produced spore mother liquor is extracted from the locusts themselves.

[0041] The spore dissolving agent of the Microsporidium locust AL200801 bacterial strain of embodiment 1 gained is diluted into the Microsporidium locust AL200801 suspension concentrate of 0.2 billion spores / ml, and is selected to be 0.2 billion spores / ml concentration by other Microsporidium locusts on the market The suspending agent product (manufacturer: Nantong Deyi Chemical Co., Ltd., date of production: May 2, 2021) made by the bacterial strain is used as a control agent, respectively recorded as the No. locust strain AL200801 group and the No. locust control group, 100 locusts per group.

[0042] The above-mentioned test locusts and their food were sprayed and inoculated with Microsporidium locust AL200801 sus...

Embodiment 3

[0048] Acute Toxicity Test

[0049] The experiment was carried out in accordance with the national standard "GB / T15670.1-2017 Toxicological Test Methods for Pesticide Registration", and the methods of rat oral, dermal, inhalation, rabbit eye and skin irritation, and guinea pig sensitization were used to treat locust microbes. The toxicity of sporozoite AL200801 was tested and the results of its virulence and irritation characteristics were compared. The experimental results are shown in Table 2.

[0050] Table 2 Acute toxicity test results

[0051]

[0052]

[0053] Note: male / female is tested on both female rats and male rats, oral and percutaneous investigation of LD 50 mg / kg, inhalation investigation LC 50 mg / kg.

[0054] As can be seen from Table 2, the Microsporidia locust AL200801 bacterial strain of the present invention is nontoxic to mammals such as mice, rabbits, guinea pigs, and death occurs when inoculated in locusts, and it can be seen that Microsporidia ...

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Abstract

The invention relates to the technical field of insect biological control, in particular to a locust microsporidia AL200801 strain as well as a biological preparation and application of the locust microsporidia AL200801 strain. The invention provides a locust microsporidia AL200801 strain, and the preservation number of the locust microsporidia AL200801 strain is CCTCC No. V202137. The locust microsporidia AL200801 strain disclosed by the invention has the advantages of high timeliness, high lethality, high sporulation rate, no toxicity and strong obligate property.

Description

technical field [0001] The invention relates to the technical field of insect biological control, in particular to a strain of Microsporidia locust AL200801 and its biological preparation and application. Background technique [0002] Locusts are major pests that seriously threaten my country's agricultural and animal husbandry production. At present, the method of controlling this pest is mainly chemical pesticide control. Although the large-scale use of chemical pesticides can effectively control locust infestation, it will also bring environmental pollution, excessive chemical pesticide residues in agricultural products, increased resistance of locusts, and increased control costs. Cause adverse consequences such as human and animal poisoning. Therefore, it is a very urgent task to adopt control methods that can replace chemical pesticides in order to achieve the goal of sustainable development. [0003] Microsporidia locusts are obligate pathogenic microorganisms of in...

Claims

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Application Information

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IPC IPC(8): C12N1/10A01N63/10A01P7/04C12R1/90
CPCC12N1/10A01N63/10Y02A50/30
Inventor 张昕然
Owner 北京嘉景生物科技有限责任公司
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