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Method for recovering and using waste fermentation broth of paenibacillus polymyxa

A technology of polymyxa spore and Bacillus, which is applied to the recycling and utilization of Paenibacillus polymyxa fermentation waste liquid, bacillus fermentation synergist and fermentation synergistic field, can solve the problem of unfavorable energy saving, emission reduction and waste of supernatant liquid components , environmental pollution and other issues, to achieve the effect of increasing spore formation rate, reducing dosage and reducing emissions

Active Publication Date: 2018-07-20
武汉科诺生物科技股份有限公司
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  • Application Information

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Problems solved by technology

In the post-treatment process of Paenibacillus polymyxa fermentation, the sludge with high specific gravity is usually collected by centrifugation, and then spray-dried to prepare Paenibacillus polymyxa bacteria powder, and the supernatant of the centrifuged fermentation liquid is usually put into the sewage treatment system as waste liquid. This treatment method not only wastes a large amount of components in the supernatant, but also causes serious pollution to the environment, which is not conducive to energy saving and emission reduction.

Method used

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  • Method for recovering and using waste fermentation broth of paenibacillus polymyxa

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Embodiment 1

[0023] After the fermentation and cultivation of Paenibacillus polymyxa, the bacteria sludge with high specific gravity is collected by centrifugation, spray-dried to prepare Paenibacillus polymyxa bacteria powder, and the supernatant of the centrifuged fermentation liquid is recycled according to the following steps:

[0024] (1) Add 0.05% chitosan by weight to the Paenibacillus polymyxa fermentation waste liquid obtained by centrifugation, stir again after 10 minutes and get the supernatant;

[0025] (2) Add 0.001% amylase by weight to the supernatant, and perform enzymatic hydrolysis for 60 minutes;

[0026] (3) Add the supernatant after enzymolysis to the Bacillus fermentation medium after passing through a strongly acidic styrene-based cation exchange column.

Embodiment 2

[0028] (1) Add 0.1% chitosan to the Paenibacillus polymyxa fermentation waste liquid obtained by centrifugation, and centrifuge again after stirring for 1 minute to get the supernatant;

[0029] (2) Add 0.005% amylase to the supernatant, and perform enzymatic hydrolysis for 15 minutes;

[0030] (3) Add the supernatant after enzymolysis to the Bacillus fermentation medium after passing through a strongly acidic styrene-based cation exchange column.

Embodiment 3

[0032] (1) Add 0.08% chitosan to the Paenibacillus polymyxa fermentation waste liquid obtained by centrifugation, stir for 5 minutes and then centrifuge again to get the supernatant;

[0033] (2) Add 0.003% amylase to the supernatant, and perform enzymatic hydrolysis for 30 minutes;

[0034] (3) Add the supernatant after enzymolysis to the Bacillus fermentation medium after passing through a strongly acidic styrene-based cation exchange column.

[0035] The treatment liquid in embodiment 1-3 is carried out physical and chemical index analysis, wherein total nitrogen adopts Kjeldahl method to detect, amino nitrogen adopts formaldehyde titration method to detect, total sugar adopts anthrone determination method, reducing sugar uses reducing sugar detector, The phosphorus-molybdenum blue colorimetric method was used for dissolving phosphorus, and the degree of protein hydrolysis refers to the ratio of amino nitrogen to total nitrogen. The test results are shown in the table belo...

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Abstract

The invention discloses a method for recovering and using waste fermentation broth of paenibacillus polymyxa. The method comprises following steps: (1) adding 0.05 to 0.1 wt% of chitosan into centrifugally obtained waste fermentation broth of paenibacillus polymyxa, stirring to carry out flocculation, carrying out centrifugation, and saving the supernate; (2) adding 0.001 to 0.005 wt% of amylase into supernate, and carrying out enzymatic hydrolysis for 15 to 60 minutes; and (3) after enzymatic hydrolysis, making the supernate go through a cation exchange column, and adding the obtained producttaken as a fermentation synergist into a bacillus fermentation culture medium. The synergist can reduce the using amount of the bacillus fermentation culture medium and increase the forming rate of spores.

Description

technical field [0001] The invention belongs to the field of microbial fermentation. It specifically relates to a method for recycling Paenibacillus polymyxa fermentation waste liquid, and the invention also relates to a Bacillus fermentation synergist and a fermentation synergist method. Background technique [0002] Paenibacillus polymyxa can produce a variety of active metabolites during liquid fermentation, such as polypeptides, lipopeptides, polysaccharides, proteins, nucleic acids, pyrazines, and phenolic substances, and most of the active substances are stable in nature. In the post-treatment process of Paenibacillus polymyxa fermentation, the sludge with high specific gravity is usually collected by centrifugation, and then spray-dried to prepare Paenibacillus polymyxa bacteria powder, and the supernatant of the centrifuged fermentation liquid is usually put into the sewage treatment system as waste liquid. This treatment method not only wastes a large amount of com...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C12N3/00C12R1/125C12R1/07C12R1/08
CPCC12N1/20C12N3/00C12P1/04
Inventor 潘渝周莉刘守德刘华梅程治国邹维万俊王猛孙刚忠胡虓周波
Owner 武汉科诺生物科技股份有限公司
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