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Bacillus subtilis and fermentation method with high yield of indoleacetic acid and high spore formation rate

A technology of Bacillus subtilis and fermentation method, which is applied in the field of Bacillus subtilis and its fermentation of high indole acetic acid and high spore formation rate, can solve the problems of low acetic acid output, unstable spore formation rate, long spore formation induction time and the like , to achieve the effect of high spore formation rate

Active Publication Date: 2021-05-25
河北冀微生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solved the problems of low yield of indole acetic acid in liquid fermentation, long spore formation induction time and unstable spore formation rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Shake flask culture

[0038] Take the Bacillus subtilis 8-32 preserved on the inclined plane, inoculate the strain in the shake flask medium with an inoculation loop, and the shake flask medium consists of the following components in parts by mass: 2% corn flour, 5% soybean meal powder, Potassium dihydrogen phosphate 0.01%, magnesium sulfate 0.01%, calcium chloride 0.01%, the balance is water; place the shake flask in a shaking incubator, the culture temperature is 30 ℃, the rotation speed is 150r / min, and the culture is 18h. bottle of seed liquid;

[0039] (2) Seed tank fermentation

[0040] The shake flask seed liquid was inoculated into the seed medium at a volume ratio of 10%, and the seed medium was composed of the following components by mass: 5% yeast extract powder, 2% peptone, 0.03% sodium chloride, 0.03% magnesium sulfate , calcium chloride 0.03%, the balance is water; control the fermentation conditions of the seed tank: the temperature is 37 ℃, the spe...

Embodiment 2

[0047] (1) Shake flask culture

[0048] Take the Bacillus subtilis 8-32 preserved on the inclined plane, inoculate the strain in the shake flask medium with an inoculating loop, and the shake flask medium consists of the following components by mass: 5% corn flour, 2% soybean meal powder, Potassium dihydrogen phosphate 0.03%, magnesium sulfate 0.03%, calcium chloride 0.03%, the balance is water; the shake flask is placed in a shaking incubator, the culture temperature is 37 ° C, the speed is 200 r / min, and the culture is 24 hours. bottle of seed liquid;

[0049] (2) Seed tank fermentation

[0050] The shake flask seed liquid was inoculated into the seed medium at a volume ratio of 5%, and the seed medium was composed of the following components by mass: 2% yeast extract powder, 5% peptone, 0.01% sodium chloride, 0.01% magnesium sulfate , calcium chloride 0.01%, the balance is water; control the fermentation conditions of the seed tank: the temperature is 30 ℃, the speed of t...

Embodiment 3

[0057] (1) Shake flask culture

[0058] Take the Bacillus subtilis 8-32 preserved on the inclined plane, inoculate the strain in the shake flask medium with an inoculation loop, and the shake flask medium consists of the following components by mass: 3.5% glucose, 3.5% soybean meal powder, 3.5% chlorine Iron 0.02%, magnesium sulfate 0.02%, calcium chloride 0.02%, the balance is water; the shake flask is placed in a shaking incubator, the culture temperature is 35 ℃, the rotation speed is 180r / min, and the culture is 20h to obtain the shake flask seeds liquid;

[0059] (2) Seed tank fermentation

[0060] The shake flask seed liquid was inoculated into the seed medium at a volume ratio of 8%, and the seed medium was composed of the following components by mass: 3.5% yeast extract powder, 3.5% peptone, 0.02% sodium chloride, and 0.02% magnesium sulfate. , calcium chloride 0.02%, the balance is water; control the fermentation conditions of the seed tank: the temperature is 35 ℃,...

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Abstract

The invention discloses bacillus subtilis and a fermentation method with high yield of indoleacetic acid and high spore formation rate, and belongs to the technical field of microbial fermentation. The bacillus subtilis is named as 8-32, is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 25, 2020, and has a preservation number of CGMCC NO.20824. The invention further discloses a preparation method of the bacillus subtilis. According to the liquid bacillus subtilis strain fermentation method, the content of bacillus subtilis reaches 2*10<10> CFU / mL or above, the content of indoleacetic acid reaches 120 mg / L or above, the spore formation rate reaches 98% or above through adoption of a method of supplementing materials, controlling fermentation conditions and treating fermentation liquor after fermentation, the high yield of indoleacetic acid can be achieved, and the spore formation rate is high. The problems of low yield of indoleacetic acid, long spore formation induction time and unstable spore formation rate in liquid fermentation are solved.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a Bacillus subtilis strain and a fermentation method with high indole acetic acid production and high spore formation rate. Background technique [0002] Bacillus subtilis (Bacillus subtilis) is a kind of Gram-positive bacteria that widely exists in the environment, and belongs to a kind of Bacillus sp. Bacillus subtilis has the functions of producing plant biomass-promoting substances, antagonistic substances, protease, amylase, cellulase, etc., and the spores produced have the characteristics of heat resistance and strong stress resistance, and have been widely used in agriculture, animal husbandry, medicine and other fields. At present, there have been some reports on the production of indoleacetic acid (IAA) by Bacillus, such as the fermentation method for indoleacetic acid produced by Bacillus in the following patents: [0003] CN106011003A adopts solid ferm...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P17/10C12R1/125
CPCC12N1/20C12P17/10
Inventor 高同国杨静朱宝成张冬冬王永攀
Owner 河北冀微生物技术有限公司
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